CAJ | 학술논문

目的探讨烟曲霉感染人支气管上皮(HBE)细胞时Toll样受体2(TLR2)对树突状细胞植物血凝素-1 (Dectin-1)表达的影响.方法建立烟曲霉感染HBE细胞模型,通过PCR、Western blot方法分别在mRNA、蛋白水平检测Dectin-1和TLR2的表达情况;沉默TLR2,复制感染模型,应用Western blot及流式细胞技术评价Dectin-1的表达变化.结果 ①烟曲霉感染后HBE细胞TLR2表达水平虽然有所升高,并在感染18h达到峰值,但与静息状态表达水平的差异无统计学意义(P＞0.05).②沉默TLR2,在烟曲霉感染HBE细胞后18 h时Dectiin1的蛋白表达受到显著抑制(P＜0.05).结论 HBE细胞静息状态下能够表达TLR2,在烟曲霉感染早期,随着时间的延长其表达有逐步增高的趋势;HBE细胞启动Dectin-1的表达可能是建立在TLR2对烟曲霉早期识别的基础上,通过下游信号通路的激活来实现的.
목적 탐토연곡매감염인지기관상피(HBE)세포시Toll양수체2(TLR2)대수돌상세포식물혈응소-1 (Dectin-1)표체적영향.방법 건립연곡매감염HBE세포모형,통과PCR、Western blot방법분별재mRNA、단백수평검측Dectin-1화TLR2적표체정황;침묵TLR2,복제감염모형,응용Western blot급류식세포기술평개Dectin-1적표체변화.결과 ①연곡매감염후HBE세포TLR2표체수평수연유소승고,병재감염18h체도봉치,단여정식상태표체수평적차이무통계학의의(P＞0.05).②침묵TLR2,재연곡매감염HBE세포후18 h시Dectiin1적단백표체수도현저억제(P＜0.05).결론 HBE세포정식상태하능구표체TLR2,재연곡매감염조기,수착시간적연장기표체유축보증고적추세;HBE세포계동Dectin-1적표체가능시건립재TLR2대연곡매조기식별적기출상,통과하유신호통로적격활래실현적.
Objective To investigate the effect of Toll like receptor 2 (TLR2) on Dectin-1 expression in HBE cells resulted from Aspergillus fumigatus stimulation.Methods The infection model in vitro was established via HBE cells exposed to Aspergillus fumigatus,and the mRNA and protein expressions of Dectin-1 and TLR2 were respectively detected by PCR and Westen blot.To explore the effect,TLR2 was silenced by siRNA and the infection model was copied.Then,the total expression of Dectin-1 was detected by Western blot and its expression on cell membrance was observed through the flow cytometry.Results TLR2 expression in HBE cells demonstrated an increasing trend after Aspergillus fumigatus stimulation,and reached a peak after 18 hours.However,the expression was not significantly different from the basal expression.Notably,the expression of Dectin-1 both in whole cell and on cell membrance was inhibited after 18 hours when TLR2 was silenced.Conclusions Our findings reveal an increasing expression of TLR2 after HBE cells exposed to Aspergillus fumigatus.And the expression of Dectin 1 in HBE cells triggered is probablely based on TLR2's early recoganization of Aspergillus fumigatus which activates the downstream signal transduction pathyway.