CAJ | 학술논문

目的通过检测结核性胸膜炎及恶性胸膜炎患者血清和胸水中血管内皮生长因子(VEGF)含量,分析VEGF在两组患者血清、胸水中的差异,探讨VEGF在二者中的意义和诊断价值.方法对确诊结核性胸膜炎和恶性胸膜炎各30例的患者在同一日留取胸水标本10 ml及静脉血5 ml,采用双抗体夹心酶联免疫吸附试验检测患者胸水及血清中VEGF水平,分析其差异及相关性.结果结核组血清和胸水VEGF检测值分别为(45.33±18.33) ng/L、(62.73±24.65) ng/L;恶性组血清和胸水VEGF检测值分别为(66.00±29.83) ng/L、(95.54±42.11) ng/L;恶性组血清及胸水中VEGF含量均高于结核组(t值分别为3.9、5.2,P值均＜0.05).VEGF在两组的血清和胸水中均呈正相关性(r值分别为0.53、0.38,P值均＜0.05).结论在结核性胸膜炎及恶性胸膜炎患者血清、胸水中VEGF水平有差异,恶性高于结核性;两组患者胸水中VEGF含量均高于血清,胸水VEGF含量随着血清VEGF含量增高而增高.检测胸腔积液患者血清和胸水中VEGF含量对结核性胸膜炎和恶性胸膜炎的诊断和鉴别诊断有一定的价值.
목적 통과검측결핵성흉막염급악성흉막염환자혈청화흉수중혈관내피생장인자(VEGF)함량,분석VEGF재량조환자혈청、흉수중적차이,탐토VEGF재이자중적의의화진단개치.방법 대학진결핵성흉막염화악성흉막염각30례적환자재동일일류취흉수표본10 ml급정맥혈5 ml,채용쌍항체협심매련면역흡부시험검측환자흉수급혈청중VEGF수평,분석기차이급상관성.결과 결핵조혈청화흉수VEGF검측치분별위(45.33±18.33) ng/L、(62.73±24.65) ng/L;악성조혈청화흉수VEGF검측치분별위(66.00±29.83) ng/L、(95.54±42.11) ng/L;악성조혈청급흉수중VEGF함량균고우결핵조(t치분별위3.9、5.2,P치균＜0.05).VEGF재량조적혈청화흉수중균정정상관성(r치분별위0.53、0.38,P치균＜0.05).결론 재결핵성흉막염급악성흉막염환자혈청、흉수중VEGF수평유차이,악성고우결핵성;량조환자흉수중VEGF함량균고우혈청,흉수VEGF함량수착혈청VEGF함량증고이증고.검측흉강적액환자혈청화흉수중VEGF함량대결핵성흉막염화악성흉막염적진단화감별진단유일정적개치.
Objective To analyse the difference and explore the diagnosis significance of vascular endothelial growth factor (VEGF) in serum and pleural fluid of patients with tuberculous pleurisy and malignant pleurisy by measuring the concentrations of VEGF in serum and pleural fluid.Methods 10 ml pleural fluid and 5 ml blood as specimen from patients with tuberculous pleurisy and malignant pleurisy (each 30 cases) were collected on the same day.The VEGF concentrations of serum and pleural fluid were measured by the enzyme-linked immunosorbent assay.The difference and correlation were analysed.Results In tuberculous pleurisy group,the VEGF levels were (45.33 ± 18.33) ng/L in serum and (62.73± 24.65) ng/L in pleural fluid.In malignant pleurisy group,the VEGF levels were (66.00±29.83) ng/L in serum and (95.54± 42.11) ng/L in pleural fluid.The VEGF levels of serum and pleural fluid in malignant pleurisy group were higher than those in tuberculous pleurisy group (t =3.9,5.2,respectively,all P ＜0.05).VEGF levels in serum and pleural fluid in the two groups showed positive correlation (r =0.53,0.38,respectively,all P ＜0.05).Conclusions The levels of VEGF in serum and pleural fluid are different between patients with tuberculous pleurisy and patients with malignant pleurisy.The serum and pleural fluid VEGF levels in patients with malignant pleurisy are higher than those in patients with tuberculous pleurisy.VEGF level of pleural fluid is higher than that of serum in the two groups.Detection of VEGF in serum and pleural fluid has a certain value in diagnosis and differential diagnosis of patients with tuberculous pleurisy and malignant pleurisy.