CAJ | 학술논문

目的建立芥子气(sulphur mustard,SM)致大鼠呼吸道损伤动物模型,观察大鼠不同时间段的肺组织学改变、BALF中蛋白含量和细胞数变化及血清炎性因子反应.方法 72只雄性大鼠随机分为SM组(32只)、丙二醇对照组(32只)和正常对照组(8只).SM组气管内注入稀释的SM 0.1 ml,丙二醇对照组气管内注入丙二醇0.1 ml,正常对照组不作处理.分别于6h、24 h、48h、72 h获取BALF、血清和肺组织标本,进行HE和免疫组化染色,光镜下观察组织学改变、炎细胞浸润,并测定肺泡间隔面积、BALF中蛋白含量和细胞数及血清炎性因子水平.结果 SM组损伤细支气管上皮细胞脱落,灶性溃疡形成;肺间质血管扩张充血,炎性细胞浸润以淋巴细胞为主;肺泡腔内出现炎性渗出物和炎细胞,BALF中蛋白含量和细胞计数48 h达高峰;血清肿瘤坏死因子α、IL-1β、IL-6水平24 h达高峰;肺泡间隔面积明显增宽,损伤程度与时间呈相关性.结论 SM(2 mg/kg)可致似ARDS组织学改变,肺内以淋巴细胞浸润为主,BALF和血清提示短暂炎性反应,且损伤程度与时间有相关性.
목적 건립개자기(sulphur mustard,SM)치대서호흡도손상동물모형,관찰대서불동시간단적폐조직학개변、BALF중단백함량화세포수변화급혈청염성인자반응.방법 72지웅성대서수궤분위SM조(32지)、병이순대조조(32지)화정상대조조(8지).SM조기관내주입희석적SM 0.1 ml,병이순대조조기관내주입병이순0.1 ml,정상대조조불작처리.분별우6h、24 h、48h、72 h획취BALF、혈청화폐조직표본,진행HE화면역조화염색,광경하관찰조직학개변、염세포침윤,병측정폐포간격면적、BALF중단백함량화세포수급혈청염성인자수평.결과 SM조손상세지기관상피세포탈락,조성궤양형성;폐간질혈관확장충혈,염성세포침윤이림파세포위주;폐포강내출현염성삼출물화염세포,BALF중단백함량화세포계수48 h체고봉;혈청종류배사인자α、IL-1β、IL-6수평24 h체고봉;폐포간격면적명현증관,손상정도여시간정상관성.결론 SM(2 mg/kg)가치사ARDS조직학개변,폐내이림파세포침윤위주,BALF화혈청제시단잠염성반응,차손상정도여시간유상관성.
Objective To establish animal model of acute lung injury induced by sulfur mustard (SM) in rats,in order to observe the histomorphologic change of lung,protein content and cell numbers in bronchoalveolar lavage fluid (BALF),and serum inflammatory factors reaction in different time phases.Methods 72 male rats were selected,then randomly divided into three groups:SM group (n =32),propylene glycol group (n =32),and control group (n =8).The rats in SM group were injected intratracheally diluted SM (2 mg/kg,0.1 ml),the rats in propylene glycol group were injected intratracheally propylene glycol 0.1 ml,and status quo was kept with the control group.BALF,serum,and lung tissue were obtained in 6h,24h,48h,and 72h respectively.HE and immunohistochemistry staining were used to observe histomorphologic change and inflammatory cell infiltration.The alveolar interval area,content of inflammatory factor in serum,total albumen and cell number in BALF were measured.Results SM group:①The epithelium cells of the damaged bronchioles were shed,and the focal ulcer formed.②Dilatating and congestive vessels were observed in pulmonary interstitial,and lymphocyte is the main element of the inflammatory infiltration.③Inflammatory exudate and inflammatory cells appeared in alveolar cavity,which the albumen content and cell number in BALF peaked in 48 hours.④The content of tumor necrosis factor-α,interleukin-1β,and interleukin-6 in serum peaked in 24 hours.⑤ The alveolar interval area widened obviously,and the damage degree was time dependent.Results in the propylene glycol group was the same with those in the control group.Conclusions SM (2 mg/kg) can induce histomorphologic change similar to acute respiratory distress syndrome.Lymphocyte is the main element of inflammatory infiltration.BALF and serum results suggest there was temporary inflammation,and the damage degree is time dependent.