CAJ | 학술논문

为了安全有效地进行基因治疗,需要把转染的基因限制在特定组织中表达.应用microRNA(miRNA)调节转基因表达,包括病毒基因表达是一个新方法.在miRNA介导的转基因表达系统中,可以将与miRNA互补的靶序列串联插入3'非编码区域,导致在miRNA高表达细胞中抑制转基因的表达.我们总结了miRNA调节病毒转基因表达系统的调节机制及在病毒疫苗中的应用.
위료안전유효지진행기인치료,수요파전염적기인한제재특정조직중표체.응용microRNA(miRNA)조절전기인표체,포괄병독기인표체시일개신방법.재miRNA개도적전기인표체계통중,가이장여miRNA호보적파서렬천련삽입3'비편마구역,도치재miRNA고표체세포중억제전기인적표체.아문총결료miRNA조절병독전기인표체계통적조절궤제급재병독역묘중적응용.
For safe and effective gene therapy,targeted tissue-restricted transgene expression is desirable.A new system which can regulate transgene expression,including viral gene expression,by exploiting microRNA (miRNA) has recently been developed.In miRNA-regulated transgene expression systems,tissue-specific miRNA recognition element are usually incorporated into the 3'-UTR of the transgene expression cassette,leading to the suppression of transgene expression in cells highly expressing the corresponding miRNA.The aim of this review is to highlight the mechanism of viral transgene expression systems for viral vaccine.