国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2014年
19期
1461-1465
,共5页
哮喘%T淋巴细胞活化衔接子%调控因子%单核苷酸多态性
哮喘%T淋巴細胞活化銜接子%調控因子%單覈苷痠多態性
효천%T림파세포활화함접자%조공인자%단핵감산다태성
Asthma%Linker for activation of T cell%Regulatory factors%Single nucleotide polymorphisms
目的 探讨支气管哮喘(简称哮喘)患者T淋巴细胞活化衔接子(LAT)基因及其上游调控因子存在(或不存在)单核苷酸多态性(SNP)位点,从而明确哮喘患者外周血T细胞中LAT及其上游调控因子LCK和ZAP-70转录水平异常是否是由于相应基因多态性引起.方法 对24例哮喘患者及24例非特应症对照者,采用直接测序法检测LAT及其上游调控子(ZAP-70、LCK)的启动子、外显子区域SNP位点出现的频率,使用统计软件SPSS 13.0将2组间SNP出现频率进行x2检验(理论频数＜5时使用Fisher's Exact Test).结果 在所有片段的测序结果中共发现15个SNP位点,与NCBI dbSNP数据库中的目标序列匹配后发现其分别为位于LAT序列上的Rs11859822、Rs7140、Rs41280846、Rs41292396、Rs1131543、Rs4788115、Rs1802141;LCK序列上的:Rs10914540和ZAP-70序列上的:Rs13034349、Rs2276645、Rs2276644、Rs2289918、Rs13002912、Rs17488834、Rs2278699.使用统计软件SPSS 13.0将两组间SNP出现频率进行x2检验(理论频数＜5时使用Fisher's Exact Test),结果显示P值均＞0.05,差异无统计学意义.结论 未于LAT基因及其上游调控因子的启动子及外显子部位找到与哮喘发病相关的SNP位点.
目的 探討支氣管哮喘(簡稱哮喘)患者T淋巴細胞活化銜接子(LAT)基因及其上遊調控因子存在(或不存在)單覈苷痠多態性(SNP)位點,從而明確哮喘患者外週血T細胞中LAT及其上遊調控因子LCK和ZAP-70轉錄水平異常是否是由于相應基因多態性引起.方法 對24例哮喘患者及24例非特應癥對照者,採用直接測序法檢測LAT及其上遊調控子(ZAP-70、LCK)的啟動子、外顯子區域SNP位點齣現的頻率,使用統計軟件SPSS 13.0將2組間SNP齣現頻率進行x2檢驗(理論頻數＜5時使用Fisher's Exact Test).結果 在所有片段的測序結果中共髮現15箇SNP位點,與NCBI dbSNP數據庫中的目標序列匹配後髮現其分彆為位于LAT序列上的Rs11859822、Rs7140、Rs41280846、Rs41292396、Rs1131543、Rs4788115、Rs1802141;LCK序列上的:Rs10914540和ZAP-70序列上的:Rs13034349、Rs2276645、Rs2276644、Rs2289918、Rs13002912、Rs17488834、Rs2278699.使用統計軟件SPSS 13.0將兩組間SNP齣現頻率進行x2檢驗(理論頻數＜5時使用Fisher's Exact Test),結果顯示P值均＞0.05,差異無統計學意義.結論 未于LAT基因及其上遊調控因子的啟動子及外顯子部位找到與哮喘髮病相關的SNP位點.
목적 탐토지기관효천(간칭효천)환자T림파세포활화함접자(LAT)기인급기상유조공인자존재(혹불존재)단핵감산다태성(SNP)위점,종이명학효천환자외주혈T세포중LAT급기상유조공인자LCK화ZAP-70전록수평이상시부시유우상응기인다태성인기.방법 대24례효천환자급24례비특응증대조자,채용직접측서법검측LAT급기상유조공자(ZAP-70、LCK)적계동자、외현자구역SNP위점출현적빈솔,사용통계연건SPSS 13.0장2조간SNP출현빈솔진행x2검험(이론빈수＜5시사용Fisher's Exact Test).결과 재소유편단적측서결과중공발현15개SNP위점,여NCBI dbSNP수거고중적목표서렬필배후발현기분별위위우LAT서렬상적Rs11859822、Rs7140、Rs41280846、Rs41292396、Rs1131543、Rs4788115、Rs1802141;LCK서렬상적:Rs10914540화ZAP-70서렬상적:Rs13034349、Rs2276645、Rs2276644、Rs2289918、Rs13002912、Rs17488834、Rs2278699.사용통계연건SPSS 13.0장량조간SNP출현빈솔진행x2검험(이론빈수＜5시사용Fisher's Exact Test),결과현시P치균＞0.05,차이무통계학의의.결론 미우LAT기인급기상유조공인자적계동자급외현자부위조도여효천발병상관적SNP위점.
Objective To investigate if there're SNPs in transcription factor linker for activation of T cell (LAT) and its upstream regulating factors (LCK,ZAP-70) and detect whether the abnormal mRNA expression of LAT,LCK and ZAP-70 genes was caused by the polymorphism.Methods Polymerase chain reaction (PCR) and straight sequencing were used to detect the SNPs in the promoter and exons of LAT and its upstream regulatory factors (LCK and ZAP-70) in 24 asthmatic patients and 24 nonallergic subjects.Then we made x2 test on the three SNP freq.by software SPSS 13.0.Results We totally found 15 SNP sites.And these SNP sites are located on Rs11859822,Rs7140,Rs41280846,Rs41292396,Rs1131543,Rs4788115,Rs1802141 of LAT series,Rs10914540 of LCK series,and Rs13034349,Rs2276645,Rs2276644,Rs2289918,Rs13002912,Rs17488834,Rs2278699 of ZAP-70 series.Later we made x2 test on the three SNP freq.by software SPSS 13.0 (made Fisher's Extact Test when the freq less than 5).The results showed that the average of P is more than 0.05.There was no statistica discrepancy.Conclusions We found none SNPs that related to asthma in the promoter and exons of LAT (linker for activation of T cell) and its upstream regulating factor.
