CAJ | 학술논문

目的探讨人端粒酶逆转录酶(hTERT)基因5'调控区单核苷酸多态性(SNP)与肺癌发生易感性的关系.方法选取2011年1月至2012年12月兰州大学第二医院病理组织学确诊的新发肺癌患者154例,男116例,女38例;平均年龄(57.3±11.6)岁;其中腺癌45例(29.2％),鳞癌55例(35.7％),小细胞癌54例(35.1％).选择同期在该院体检中心性别、年龄匹配的健康体检者222名作为对照,男174名,女48名,平均年龄(56.4±8.4)岁.采用ABI Prism SNaPshot方法,检测hTERT基因8个SNP位点(rs2735846、rs2735948、rs62329683、rs2736106、rs3888705、rs2736108、rs2736109和rs2735940)基因型,对SNP位点进行Hardy-Weiberg平衡检验,并计算各SNP位点等位基因频率及OR值.结果除rs2736106外,7个hTERT基因5'SNP位点的基因型分布均符合Hardy-Weiberg平衡.其中rs2735846位点CG+CC基因型与GG基因型(即显性遗传模式)在病例组和对照组中具有显著的统计学差异(P ＜0.05).结论 hTERT基因rs2735846多态性与肺癌易感性有显著相关性,其等位基因C是肺癌的易感基因.
목적 탐토인단립매역전록매(hTERT)기인5'조공구단핵감산다태성(SNP)여폐암발생역감성적관계.방법 선취2011년1월지2012년12월란주대학제이의원병리조직학학진적신발폐암환자154례,남116례,녀38례;평균년령(57.3±11.6)세;기중선암45례(29.2％),린암55례(35.7％),소세포암54례(35.1％).선택동기재해원체검중심성별、년령필배적건강체검자222명작위대조,남174명,녀48명,평균년령(56.4±8.4)세.채용ABI Prism SNaPshot방법,검측hTERT기인8개SNP위점(rs2735846、rs2735948、rs62329683、rs2736106、rs3888705、rs2736108、rs2736109화rs2735940)기인형,대SNP위점진행Hardy-Weiberg평형검험,병계산각SNP위점등위기인빈솔급OR치.결과 제rs2736106외,7개hTERT기인5'SNP위점적기인형분포균부합Hardy-Weiberg평형.기중rs2735846위점CG+CC기인형여GG기인형(즉현성유전모식)재병례조화대조조중구유현저적통계학차이(P ＜0.05).결론 hTERT기인rs2735846다태성여폐암역감성유현저상관성,기등위기인C시폐암적역감기인.
Objective To study the association between single nucleotide polymorphism (SNP) of human telomerase reverse transcriptase (hTERT) gene of 5'-upstream sequence and lung cancer.Methods From January 2011 to December 2012,blood samples and clinical data of 154 patients with lung cancer newly diagnosed by histopathology in the Second Hospital of Lanzhou University were collected,including 116 males and 38 females.Mean age was (57.3 ± 11.6) years old.There were 45 adenocarcinomas (29.2％),55 squamous carcinomas (35.7％),and 54 small cell lung cancers (35.1％).Matching controls included 174 males and 48 females,aging (56.4±8.4) years old.The genotypes of SNP (rs2735846,rs2735948,rs62329683,rs2736106,rs3888705,rs2736108,rs2736109,and rs2735940) in hTERT gene were determined with ABI Prism SNaPshot.And then Hardy-Weinberg equilibrium test,the allelic frequency,and odds ratio were calculated.Results Except rs2736106,other seven SNP of hTERT were in Hardy-Weinberg equilibrium in control group.In dominant model,the CG + CC and GG distribution of rs2735846 in lung cancer patients was significantly different from that in controls.Conclusions The results suggests that the rs2735846 SNP in 5'UTR of hTERT gene is associated with the risk of lung cancer.The allele C is the risk factor of lung cancer.