国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2012年
5期
301-305
,共5页
宋鹏%吴南翔%楼建林%金凌之%高明%谭玉凤%宋杨%王禹%刘克澄
宋鵬%吳南翔%樓建林%金凌之%高明%譚玉鳳%宋楊%王禹%劉剋澄
송붕%오남상%루건림%금릉지%고명%담옥봉%송양%왕우%류극징
多氯联苯化合物%基因表达%逆转录聚合酶链反应
多氯聯苯化閤物%基因錶達%逆轉錄聚閤酶鏈反應
다록련분화합물%기인표체%역전록취합매련반응
Polychlorinated biphenyls%Gene expression%Reverse transcriptase polymerase chain reaction
目的 研究多氯联苯暴露人群外周血相关基因表达改变.方法 采集32例电子垃圾拆解地居民和48例对照人群外周血样,分离提取总RNA,用实时荧光定量PCR检测CCDC92、CCL22、GZMK、MTDH、STK38L、TMEM175等6个基因的mRNA表达水平.结果 根据双标准曲线法,拆解地居民CCL22基因的表达水平为(4.51±2.37)％,低于对照组的(6.76±4.82) ％(t=2.451,P ＜0.05).根据2-△△Ct法,两者的表达水平分别为(0.54±0.28)倍和(0.81±0.58)倍(t=2.480,P＜0.05).根据双标准曲线法,GZMK和MTDH基因的表达水平高于对照组(t=-2.036、-2.228,P＜0.05).根据2-△△Ct法,两者的表达水平也高于对照组(t=-2.035、-2.223,P＜0.05).两种定量方法存在较好的相关性(r=1.000,P＜0.01).结论 CCL22、GZMK和MTDH三个基因在多氯联苯暴露人群中表达异常,可用作多氯联苯暴露人群的效应标志物.
目的 研究多氯聯苯暴露人群外週血相關基因錶達改變.方法 採集32例電子垃圾拆解地居民和48例對照人群外週血樣,分離提取總RNA,用實時熒光定量PCR檢測CCDC92、CCL22、GZMK、MTDH、STK38L、TMEM175等6箇基因的mRNA錶達水平.結果 根據雙標準麯線法,拆解地居民CCL22基因的錶達水平為(4.51±2.37)％,低于對照組的(6.76±4.82) ％(t=2.451,P ＜0.05).根據2-△△Ct法,兩者的錶達水平分彆為(0.54±0.28)倍和(0.81±0.58)倍(t=2.480,P＜0.05).根據雙標準麯線法,GZMK和MTDH基因的錶達水平高于對照組(t=-2.036、-2.228,P＜0.05).根據2-△△Ct法,兩者的錶達水平也高于對照組(t=-2.035、-2.223,P＜0.05).兩種定量方法存在較好的相關性(r=1.000,P＜0.01).結論 CCL22、GZMK和MTDH三箇基因在多氯聯苯暴露人群中錶達異常,可用作多氯聯苯暴露人群的效應標誌物.
목적 연구다록련분폭로인군외주혈상관기인표체개변.방법 채집32례전자랄급탁해지거민화48례대조인군외주혈양,분리제취총RNA,용실시형광정량PCR검측CCDC92、CCL22、GZMK、MTDH、STK38L、TMEM175등6개기인적mRNA표체수평.결과 근거쌍표준곡선법,탁해지거민CCL22기인적표체수평위(4.51±2.37)％,저우대조조적(6.76±4.82) ％(t=2.451,P ＜0.05).근거2-△△Ct법,량자적표체수평분별위(0.54±0.28)배화(0.81±0.58)배(t=2.480,P＜0.05).근거쌍표준곡선법,GZMK화MTDH기인적표체수평고우대조조(t=-2.036、-2.228,P＜0.05).근거2-△△Ct법,량자적표체수평야고우대조조(t=-2.035、-2.223,P＜0.05).량충정량방법존재교호적상관성(r=1.000,P＜0.01).결론 CCL22、GZMK화MTDH삼개기인재다록련분폭로인군중표체이상,가용작다록련분폭로인군적효응표지물.
Objective To investigate expression changes of related genes in peripheral blood samples collected from residents exposed to polychlorinated biphenyls (PCBs).Methods Peripheral blood samples were collected from 32 residents in an e-waste recycling site and 48 heaalthy controls.Total RNA was isolated and the expression of CCDC92,CCL22,GZMK,MTDH,STK38L and TMEM175 were detected by quantitative real-time PCR.Results The expression level of CCL22 in the recycling site residents was significantly lower than that of controls by double-standard curves method [(4.51 ± 2.37) ％:(6.76 ± 4.82) ％,t =2.451,P ＜ 0.05).By 2-△△Ct method,the expression level in two group were (0.54 ± 0.28) times and (0.81 ± 0.58) times (t =2.480,p ＜ 0.05).But the expression levels of GZMK and MTDH were markedly enhanced in exposure group by double-standard curves method (t =-2.036,-2.228,P ＜ 0.05).By 2-△△Ct method,their expression levels were higher than control group (t =-2.035,-2.223,P ＜ 0.05).Good correlations were found between two quantification methods (r =1.000,P ＜ 0.01).Conclusions CCL22,GZMK and MTDH which expressed abnormally in residents in a PCBs pollution site may be used as effect biomarkers for people exposed to PCBs.
