国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2013年
1期
13-16
,共4页
赵允武%李涛%王禹%楼建林
趙允武%李濤%王禹%樓建林
조윤무%리도%왕우%루건림
二甲基甲酰胺%肝功能%DNA损伤%谷胱甘肽
二甲基甲酰胺%肝功能%DNA損傷%穀胱甘肽
이갑기갑선알%간공능%DNA손상%곡광감태
Dimethylfomamide%Liver function%DNA damage%Glutathione
目的 研究二甲基甲酰胺(DMF)暴露工人的血清ALT、外周血DNA损伤及血浆谷胱甘肽(GSH)含量,并分析其与暴露水平之间的关系.方法 选择50名DMF作业工人为暴露组,同一地区不接触DMF的50名工人为对照组,测定暴露工人班末尿中甲基甲酰胺(NMF)的含量,并检测血清ALT含量及血浆GSH含量,用彗星试验检测外周血DNA损伤.结果 暴露组ALT、DNA损伤分别为(29.66±21.76) U/L和5.36±3.33,均显著高于对照组(t=3.067、4.512,P＜0.01),暴露组GSH含量为(6.38±1.70) μmol/L,显著低于对照组(t＝-6.811,P＜0.01).对照组、低暴露组(尿NMF 0 ～ 10 mg/L)和高暴露组(尿NMF＞ 10 mg/L)的ALT含量,DNA损伤及GSH含量差异均有统计学意义(F=5.46、12.27、23.42,P＜0.01),两两比较发现低暴露组和高暴露组ALT、DNA损伤均显著高于对照组(P＜ 0.05,P＜0.01),而GSH含量则显著低于对照组(P＜0.01),低、高暴露组三个指标之间差异均无统计学意义(P均＞0.05).工人尿中NMF含量与ALT、DNA损伤及GSH含量之间存在显著相关性(r=0.321、0.449、-0.544,P均＜0.01).结论 DMF可引起作业工人的肝功能损伤及外周血DNA损伤,可能和GSH含量降低有关.
目的 研究二甲基甲酰胺(DMF)暴露工人的血清ALT、外週血DNA損傷及血漿穀胱甘肽(GSH)含量,併分析其與暴露水平之間的關繫.方法 選擇50名DMF作業工人為暴露組,同一地區不接觸DMF的50名工人為對照組,測定暴露工人班末尿中甲基甲酰胺(NMF)的含量,併檢測血清ALT含量及血漿GSH含量,用彗星試驗檢測外週血DNA損傷.結果 暴露組ALT、DNA損傷分彆為(29.66±21.76) U/L和5.36±3.33,均顯著高于對照組(t=3.067、4.512,P＜0.01),暴露組GSH含量為(6.38±1.70) μmol/L,顯著低于對照組(t＝-6.811,P＜0.01).對照組、低暴露組(尿NMF 0 ～ 10 mg/L)和高暴露組(尿NMF＞ 10 mg/L)的ALT含量,DNA損傷及GSH含量差異均有統計學意義(F=5.46、12.27、23.42,P＜0.01),兩兩比較髮現低暴露組和高暴露組ALT、DNA損傷均顯著高于對照組(P＜ 0.05,P＜0.01),而GSH含量則顯著低于對照組(P＜0.01),低、高暴露組三箇指標之間差異均無統計學意義(P均＞0.05).工人尿中NMF含量與ALT、DNA損傷及GSH含量之間存在顯著相關性(r=0.321、0.449、-0.544,P均＜0.01).結論 DMF可引起作業工人的肝功能損傷及外週血DNA損傷,可能和GSH含量降低有關.
목적 연구이갑기갑선알(DMF)폭로공인적혈청ALT、외주혈DNA손상급혈장곡광감태(GSH)함량,병분석기여폭로수평지간적관계.방법 선택50명DMF작업공인위폭로조,동일지구불접촉DMF적50명공인위대조조,측정폭로공인반말뇨중갑기갑선알(NMF)적함량,병검측혈청ALT함량급혈장GSH함량,용혜성시험검측외주혈DNA손상.결과 폭로조ALT、DNA손상분별위(29.66±21.76) U/L화5.36±3.33,균현저고우대조조(t=3.067、4.512,P＜0.01),폭로조GSH함량위(6.38±1.70) μmol/L,현저저우대조조(t＝-6.811,P＜0.01).대조조、저폭로조(뇨NMF 0 ～ 10 mg/L)화고폭로조(뇨NMF＞ 10 mg/L)적ALT함량,DNA손상급GSH함량차이균유통계학의의(F=5.46、12.27、23.42,P＜0.01),량량비교발현저폭로조화고폭로조ALT、DNA손상균현저고우대조조(P＜ 0.05,P＜0.01),이GSH함량칙현저저우대조조(P＜0.01),저、고폭로조삼개지표지간차이균무통계학의의(P균＞0.05).공인뇨중NMF함량여ALT、DNA손상급GSH함량지간존재현저상관성(r=0.321、0.449、-0.544,P균＜0.01).결론 DMF가인기작업공인적간공능손상급외주혈DNA손상,가능화GSH함량강저유관.
Objective To analyze serum ALT,DNA damage of peripheral blood cells and glutathione(GSH)level in plasma from dimethyformamide (DMF) exposed workers and to investigate the relationship between these parameters and the exposure levels of DMF.Methods There were 50 DMF exposed workers and 50 non-DMF exposed workers enrolled in the study.The levels of N-methylformamide (NMF) in end-shift urine samples were detected.The contents of ALT in serum and GSH in plasma were measured.DNA damage in peripheral blood cells was detected by comet assay.Results The levels of ALT and DNA damage in exposed group were (29.66± 21.76) U/L and 5.36± 3.33,and were significantly higher than those in the control group(t =3.067,4.512,P ＜ 0.01).The GSH content in exposed group was (6.38 ± 1.70) μmol/L,and was lower than that in the control (t =-6.811,P ＜ 0.01).The exposed group was divided into low-dose (NMF in urine:0～ 10 mg/L) and high-dose (NMF in urine ＞ 10 mg/L) groups based on the average level of NMF in urine.There were significant differences of the levels of ALT,DNA damage and GSH among control group,low-dose group and high-dose group (F =5.46,12.27,23.42,P ＜ 0.01).The levels of ALT and DNA damage in each DMF exposed group were still higher than the control (P ＜ 0.05,P ＜ 0.01),and the GSH content showed a reversed result (P ＜ 0.01).The three parameters showed no significant difference between low-dose and highdose groups.There were good correlations between the level of NMF and the levels of ALT,DNA damage,GSH,respectively (r =0.321,0.449,-0.544,P ＜ 0.01).Conclusions DMF may result in liver damage and DNA damage to humans,which might be related to decreased GSH content in plasma.
