国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2013年
2期
106-109
,共4页
整合子%整合酶%耐药性
整閤子%整閤酶%耐藥性
정합자%정합매%내약성
Integrons%Integrase%Antibiotic resistance
目的 了解临床分离弗氏柠檬酸杆菌中整合子的分布,分析整合子与细菌耐药性的关系.方法 选取2011年7月至2013年2月非重复分离自浙江省人民医院就诊患者临床样本中的37株弗氏柠檬酸杆菌,用聚合酶链反应(PCR)检测菌株第Ⅰ、Ⅱ、Ⅲ类整合子.抗生素药敏试验采用微量肉汤稀释法.结果 37株弗氏柠檬酸杆菌中,有14株(37.8％)检测到第Ⅰ类整合子,未检出第Ⅱ类和第Ⅲ类整合子.对15种常用抗生素的耐药性检测显示,第Ⅰ类整合子阳性菌株对复方磺胺甲(噁)唑及环丙沙星的耐药率高于第Ⅰ类整合子阴性菌株(P均＜0.05);对其他抗生素的耐药性,第Ⅰ类整合子阳性和阴性菌株间差异无统计学意义(P均＞0.05).结论 第Ⅰ类整合子在弗氏柠檬酸杆菌中分布普遍.第Ⅰ类整合子与复方磺胺甲(噁)唑及环丙沙星的耐药性密切相关.
目的 瞭解臨床分離弗氏檸檬痠桿菌中整閤子的分佈,分析整閤子與細菌耐藥性的關繫.方法 選取2011年7月至2013年2月非重複分離自浙江省人民醫院就診患者臨床樣本中的37株弗氏檸檬痠桿菌,用聚閤酶鏈反應(PCR)檢測菌株第Ⅰ、Ⅱ、Ⅲ類整閤子.抗生素藥敏試驗採用微量肉湯稀釋法.結果 37株弗氏檸檬痠桿菌中,有14株(37.8％)檢測到第Ⅰ類整閤子,未檢齣第Ⅱ類和第Ⅲ類整閤子.對15種常用抗生素的耐藥性檢測顯示,第Ⅰ類整閤子暘性菌株對複方磺胺甲(噁)唑及環丙沙星的耐藥率高于第Ⅰ類整閤子陰性菌株(P均＜0.05);對其他抗生素的耐藥性,第Ⅰ類整閤子暘性和陰性菌株間差異無統計學意義(P均＞0.05).結論 第Ⅰ類整閤子在弗氏檸檬痠桿菌中分佈普遍.第Ⅰ類整閤子與複方磺胺甲(噁)唑及環丙沙星的耐藥性密切相關.
목적 료해림상분리불씨저몽산간균중정합자적분포,분석정합자여세균내약성적관계.방법 선취2011년7월지2013년2월비중복분리자절강성인민의원취진환자림상양본중적37주불씨저몽산간균,용취합매련반응(PCR)검측균주제Ⅰ、Ⅱ、Ⅲ류정합자.항생소약민시험채용미량육탕희석법.결과 37주불씨저몽산간균중,유14주(37.8％)검측도제Ⅰ류정합자,미검출제Ⅱ류화제Ⅲ류정합자.대15충상용항생소적내약성검측현시,제Ⅰ류정합자양성균주대복방광알갑(오)서급배병사성적내약솔고우제Ⅰ류정합자음성균주(P균＜0.05);대기타항생소적내약성,제Ⅰ류정합자양성화음성균주간차이무통계학의의(P균＞0.05).결론 제Ⅰ류정합자재불씨저몽산간균중분포보편.제Ⅰ류정합자여복방광알갑(오)서급배병사성적내약성밀절상관.
Objective To investigate the distribution of integrons in clinical Citrobacterfreundii strains and analyze the relationship between integrons and antibiotic resistance.Methods A total of 37 non-repeated clinical isolates of Citrobacter freundii from Zhejiang Provincial People's Hospital were selected from July 2011 to February 2013.The types of the interons(intIl、intI2、intI3) were identified by PCR and the drug-sensitivity tests were carried out by broth dilution method.Results Among 37 non-repeated isolates of Citrobacter freundii,intIl was detected in 14 isolates (37.8 ％),intI2 and intI3 genes were not detected.After drug-resistance test of 15 common used antibiotics,the intI1-positive strains showed higher resistant rates of trimethoprim-sulfamethoxazole and ciprofloxacin than the intIl-negative strains (P all ＜ 0.05).For resistance rates of the other antibiotics,there was no difference between theintIl-positive and intIl-negative strains(P all ＞ 0.05).Conclusions In this study,the class Ⅰ integron is extensively found in clinical isolates of Citrobater freundii.Integrons play an important role in drug resistance of trimethoprim-sulfamethoxazole and ciprofloxacin.
