CAJ | 학술논문

目的利用邻苯二甲酸二丁酯(DBP)胚胎晚期暴露导致子代SD胎鼠发生尿道下裂的动物模型,检测波形蛋白(Vimentin)在尿道下裂与正常雄性胎鼠阴茎组织中的差异表达,以进一步探讨DBP诱导尿道下裂发生的作用机制.方法妊娠14～18d SD大鼠20只,随机分为两组,实验组和对照组分别给予DBP和大豆油800mg/(kgod)灌胃,在妊娠19d (GD19),提取雄性胎鼠阴茎组织,用免疫印迹法检测Vimentin 蛋白的表达,用免疫组织化学方法分析其定位及染色情况.结果 Vimentin蛋白在尿道下裂雄性胎鼠中的相对表达量为0.583±0.031 (n =10),在对照组的相对表达量为0.702±0.054 (n =10),差异有统计学意义(P＜0.05).Vimentin蛋白主要定位于尿道上皮细胞,尿道下裂胎鼠染色强度较对照组明显减弱.结论孕晚期大鼠暴露于DBP后改变了雄性胎鼠尿道组织中Vimentin的表达,影响了尿道上皮细胞的迁移和尿生殖褶的融合,这可能是尿道下裂发生的原因之一.
목적 이용린분이갑산이정지(DBP)배태만기폭로도치자대SD태서발생뇨도하렬적동물모형,검측파형단백(Vimentin)재뇨도하렬여정상웅성태서음경조직중적차이표체,이진일보탐토DBP유도뇨도하렬발생적작용궤제.방법 임신14～18d SD대서20지,수궤분위량조,실험조화대조조분별급여DBP화대두유800mg/(kgod)관위,재임신19d (GD19),제취웅성태서음경조직,용면역인적법검측Vimentin 단백적표체,용면역조직화학방법분석기정위급염색정황.결과 Vimentin단백재뇨도하렬웅성태서중적상대표체량위0.583±0.031 (n =10),재대조조적상대표체량위0.702±0.054 (n =10),차이유통계학의의(P＜0.05).Vimentin단백주요정위우뇨도상피세포,뇨도하렬태서염색강도교대조조명현감약.결론 잉만기대서폭로우DBP후개변료웅성태서뇨도조직중Vimentin적표체,영향료뇨도상피세포적천이화뇨생식습적융합,저가능시뇨도하렬발생적원인지일.
Objectives To estimate the differential expression of vimentin protein in the penis of hypospadiac rats induced by maternal exposure to DBP to explore molecular mechanism of hypospadias.Methods From gestation day (GD) 14 to GD18,twenty pregnant rats were randomly separated into two groups which were given DBP and soybean oil gavage at the dose of 800mg/kgod).On GD19,the expression of vimentin was analyzed by Western blotting and immunohistochemistry.Results The expression of vimentin protein had statistical difference (P ＜ 0.05).Vimentin protein predominantly located in cytoplasm of epithelium ; the control group staining intensity was stronger than that in experimental group.Conclusions The development of hypospadias may be involved in the interference of vimentin protein expression by DBP.