CAJ | 학술논문

目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)对大鼠急性肾缺血再灌注损伤(ischemia-reperfusion injury,IR/I)的保护作用及机制.方法 21只健康雄性S-D大鼠随机分为假手术组(sham组)、IR/I组和rhEPO预处理组.双侧肾蒂夹闭45min后再灌注,建立大鼠IR/I模型.术后24h收集血液和肾脏标本,观察肾功能、肾脏病理和大鼠死亡情况；ELISA法检测血清血管内皮生长因子(VEGF)的水平；免疫组织化学法观察肾脏血红素加氧酶-1(HO-1)的表达.结果再灌注24h后,rhEPO预处理组Scr和BUN水平明显低于IR/I组(P＜0.01)；IR/I组出现肾小管上皮细胞广泛性坏死,rhEPO预处理组肾脏病理损伤程度较IR/I组明显减轻(P＜0.01)；IR/I组大鼠死亡率为28.6％(2/7),rhE-PO预处理组死亡率为0(0/7),Sham组死亡率为0(0/7).rhEPO预处理组血清VEGF水平明显升高、肾脏HO-1蛋白表达明显增加,均高于IR/I组及sham组(P＜0.01).结论 rhEPO对急性肾脏IR/I有较好的保护作用,该作用可能是通过抗氧化、促进肾小管上皮细胞再生等的协同机制来实现.
목적 탐토중조인촉홍세포생성소(recombinant human erythropoietin,rhEPO)대대서급성신결혈재관주손상(ischemia-reperfusion injury,IR/I)적보호작용급궤제.방법 21지건강웅성S-D대서수궤분위가수술조(sham조)、IR/I조화rhEPO예처리조.쌍측신체협폐45min후재관주,건립대서IR/I모형.술후24h수집혈액화신장표본,관찰신공능、신장병리화대서사망정황；ELISA법검측혈청혈관내피생장인자(VEGF)적수평；면역조직화학법관찰신장혈홍소가양매-1(HO-1)적표체.결과 재관주24h후,rhEPO예처리조Scr화BUN수평명현저우IR/I조(P＜0.01)；IR/I조출현신소관상피세포엄범성배사,rhEPO예처리조신장병리손상정도교IR/I조명현감경(P＜0.01)；IR/I조대서사망솔위28.6％(2/7),rhE-PO예처리조사망솔위0(0/7),Sham조사망솔위0(0/7).rhEPO예처리조혈청VEGF수평명현승고、신장HO-1단백표체명현증가,균고우IR/I조급sham조(P＜0.01).결론 rhEPO대급성신장IR/I유교호적보호작용,해작용가능시통과항양화、촉진신소관상피세포재생등적협동궤제래실현.
Objectives To investigate protective effect of recombinant human erythropeietin (rhEPO) on acute renal ischemia-reperfusion injury(IR/I) in rats.Methods Twenty one male S-D rats were randomly divided into 3 groups:Sham,IR/I,rhEPO(injection via intraperitoneal 2 h before surgery at the dosages of 1000U/kg).Rat model of renal IR/I was established with clamping both pedicles for 45 min followed by reperfusion.Blood sample and kidneys were collected at indicated times.Serum creatinine levels,urea nitrogen,histological change and mortality were observed throughout the study.ELISA was used to measure the levels of serum vascular endothelial growth factor(VEGF).The expression of heme oxygenase-1 (HO-1) in kidney observed by Immunohistochemical.Results Extensive proximal tubular necrosis,functional impairment and higher mortality were found repeffusion after 24 hours in IR/I group (P ＜ 0.01).Elevated serum VEGF levels and increased expression of HO-1 protein in kidney were significantly higher than IR/I group and sham group(P ＜ 0.01).Conclusions thEPO can attenuate renal IR/I.The protection mechanisms may be through the anti-oxidation and to promote renal tubular epithelial cell regeneration.