CAJ | 학술논문

目的 HPV与膀胱癌的研究存在很多争论,尤其是HPV在膀胱癌组织中的检出率有很大差异,0％到81％不等,本课题旨在明确HPV、P53与膀胱移行细胞癌发生的关系.方法 PCR体外扩增和DNA反向点杂交相结合的DNA芯片技术测定39例膀胱移行细胞癌中高危型HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83、MM4；低危型HPV6、11、42、43、44等亚型的感染情况,同时用免疫组化测定P53的表达情况.结果膀胱癌组织的HPV总体阳性率为23.1％,HPV的亚型主要为11,16,其中有3例为HPV11和HPV16的混合感染,有一例检测到HPV18的亚型18,HPV16最常见,占66.7％.HPV的感染与P53表达呈正相关,与肿瘤分级无关.结论膀胱移行细胞癌发生与HPV感染的确存在明显的相关性,其中HPV亚型以HPV16为最常见,HPV11也是诱发肿瘤的亚型之一,HPV诱发肿瘤的原因可能是干扰抑癌基因P53功能,从而诱发肿瘤发生.
목적 HPV여방광암적연구존재흔다쟁론,우기시HPV재방광암조직중적검출솔유흔대차이,0％도81％불등,본과제지재명학HPV、P53여방광이행세포암발생적관계.방법 PCR체외확증화DNA반향점잡교상결합적DNA심편기술측정39례방광이행세포암중고위형HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83、MM4；저위형HPV6、11、42、43、44등아형적감염정황,동시용면역조화측정P53적표체정황.결과 방광암조직적HPV총체양성솔위23.1％,HPV적아형주요위11,16,기중유3례위HPV11화HPV16적혼합감염,유일례검측도HPV18적아형18,HPV16최상견,점66.7％.HPV적감염여P53표체정정상관,여종류분급무관.결론 방광이행세포암발생여HPV감염적학존재명현적상관성,기중HPV아형이HPV16위최상견,HPV11야시유발종류적아형지일,HPV유발종류적원인가능시간우억암기인P53공능,종이유발종류발생.
Objectives There are many issues in the relationship between transional cell carcinoma and HPV as well as P53,especially,The prevalence of HPV in TCC is still inconsistent,so,It is important to study definitely the relationship between transional cell carcinoma and HPV as well as P53.Methods HPVs in 39 Cases of transional cell carcinoma were analyzed by PCR and Reverse hybridization in situ.The HPVs included high risk types HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83、MM4 ; low risk types:HPV6、11、42、43、44,at that time,P53 gene in these tissue were detected by immunochistochemical method.Results Total Positive rate of HPV in TCC of bladder was 23.1％,HPV16 and HPV11 were major types,HPV11 and HPV16 were both found out in 3 cases.HPV16 is the best common type,Its proportion is 66.7％.There was a positive relativity between HPV and P53,but the relativity without grade of TCC.Conclusions HPV16 and HPV11 can be a factor leading to occur TCC of bladder,It could be reason that Hives interfere in function of P53.