CAJ | 학술논문

目的探讨应用前列腺组织蛋白提纯液(PTHS)辅以弗氏完全佐剂(FCA)和百白破疫苗(PDT),成功建立慢性非细菌性前列腺炎(CNP)大鼠模型所需的时间周期.方法取4个月龄雄性SD大鼠10只,麻醉处死后在无菌条件下剖腹取前列腺组织,高速离心制作PTHS,用微量紫外分光光度计测定前列腺组织蛋白含量,再以0.1 mol/LpH7.4的PBS缓冲液调节蛋白浓度至20 mg/ml.再将PTHS与FCA等体积混合成混悬液.另取2个月龄SD大鼠48只,随机分为6组,每组8只.其中5组为实验组(1周组、2周组、4周组、6周组、8周组),每只大鼠皮内多点注射PTHS与FCA的混悬液1.0ml,同时腹腔注射PDT 0.5ml,分别于1、2、4、6、8周处死后取前列腺组织观察大体形态和光镜病理特征.另1组为对照组,同法注射等量生理盐水,并于第8周处死后观察前列腺特征.结果对照组大鼠前列腺大体形态正常,光镜下未见炎症表现；1周组大鼠前列腺也无明显炎症表现；2周组大鼠前列腺组织轻度充血水肿,腺体周围可见散在的淋巴细胞浸润；4周组大鼠前列腺结构出现轻中度破坏,间质、腺体内和腺体周围均出现较多的淋巴细胞浸润；6周组大鼠前列腺结构破坏严重,间质、腺体内和腺体周围有弥漫的淋巴样组织增生和淋巴、单核细胞等慢性炎细胞浸润,提示建模成功；8周组大鼠炎症情况与6周组大鼠类似.结论应用同源大鼠的PTHS辅以双重免疫佐剂(FCA和PDT)经过6周的时间可以成功建立CNP大鼠模型.
목적 탐토응용전렬선조직단백제순액(PTHS)보이불씨완전좌제(FCA)화백백파역묘(PDT),성공건립만성비세균성전렬선염(CNP)대서모형소수적시간주기.방법 취4개월령웅성SD대서10지,마취처사후재무균조건하부복취전렬선조직,고속리심제작PTHS,용미량자외분광광도계측정전렬선조직단백함량,재이0.1 mol/LpH7.4적PBS완충액조절단백농도지20 mg/ml.재장PTHS여FCA등체적혼합성혼현액.령취2개월령SD대서48지,수궤분위6조,매조8지.기중5조위실험조(1주조、2주조、4주조、6주조、8주조),매지대서피내다점주사PTHS여FCA적혼현액1.0ml,동시복강주사PDT 0.5ml,분별우1、2、4、6、8주처사후취전렬선조직관찰대체형태화광경병리특정.령1조위대조조,동법주사등량생리염수,병우제8주처사후관찰전렬선특정.결과 대조조대서전렬선대체형태정상,광경하미견염증표현；1주조대서전렬선야무명현염증표현；2주조대서전렬선조직경도충혈수종,선체주위가견산재적림파세포침윤；4주조대서전렬선결구출현경중도파배,간질、선체내화선체주위균출현교다적림파세포침윤；6주조대서전렬선결구파배엄중,간질、선체내화선체주위유미만적림파양조직증생화림파、단핵세포등만성염세포침윤,제시건모성공；8주조대서염증정황여6주조대서유사.결론 응용동원대서적PTHS보이쌍중면역좌제(FCA화PDT)경과6주적시간가이성공건립CNP대서모형.
Objectives To investigate the period required to establish a rat model of chronic nonbacterial prostatitis(CNP) by immunized with syngeneic prostate tissue homogenate supemate (PTHS)plus Fround's complete adjuvant(FCA) and pertussis diphtheria tetanus vaccine(PDT).Methods The prostate tissues of 10 of 4-month -old male SD rats were taken out under aseptic condition,and PTHS was made through high-speed centrifugation.The concentration of PTHS was determined with micro UV spectrophotometer and diluted into 20 mg/ml then.Another 48 male SD rats of 4 months old were randomly divided into 6 groups with 8 rats each.Five groups were CNP group,including 1-week group,2-week group,4-week group,6-week group and 8-week group.Each rat of group CNP was immunized with 1.0 ml PTHS emulsified by isopyknic FCA intradermally in the multiple points,and simultaneously immunized with 0.5 ml PDT vaccine intraperitoneally.Each rat of control group was injected with equivalent normal saline in the same way.The rats were sacrificed at 1,2,4,6 and 8 weeks after immunization in CNP group,and 8 weeks in control group.Then,The prostatic tissues were harvested under aseptic condition and examined macropathologically and histologically for degree of inflammation.Results Macroscopic and histological features of prostate tissues of control group were normal.The prostatic manifestations of 1-week group were nearly normal too.But in the 2-week group,the prostate tissues were mild hyperemia with scattered lymphocytic infiltration around the glands.In the 4-week group,the prostate tissues were moderate hyperemia,with much lymphocytic infiltration within or around the acini or ducts.In the 6-week group,the prostate tissues were serious congestion and edema,adhesion with surrounding tissues,aneretic prostate capsule and so on.Histologically,the prostatic tissues were characterized by lymphoid tissue proliferation and chronic inflammatory cell infiltration in the stromal connective tissue around the acini or ducts,suggesting success of modeling.The inflammatory conditions of the 4-week group rats were similar to the rats of the 6-week group.Conclusions Six weeks were needed to establish a rat model of CNP by immunized with PTHS plus FCA and PDT.