国际泌尿系统杂志
國際泌尿繫統雜誌
국제비뇨계통잡지
INTERNATIONAL JOURNAL OF UROLOGY AND NEPHROLOGY
2014年
3期
344-348
,共5页
刘成益%徐鹏程%陈德钢%范欣欢
劉成益%徐鵬程%陳德鋼%範訢歡
류성익%서붕정%진덕강%범흔환
前列腺肿瘤%前列腺增生%自噬%细胞凋亡
前列腺腫瘤%前列腺增生%自噬%細胞凋亡
전렬선종류%전렬선증생%자서%세포조망
Prostatic Neoplasms%Prostatic Hyperplasia%Autophagy%Apoptosis
目的 检测良性前列腺增生、前列腺癌标本中自噬相关基因Beclin-1、LC3的表达及其与凋亡基因P53、Bcl-2的关系,并探讨其意义.方法 分别取34例前列腺癌组织和50例良性前列腺增生组织(术前均未服5α-还原酶抑制剂),采用Western blot方法检测其Beclin-1和LC3的表达.再将96例良性前列腺增生病理标本分为服药组(服用5α-还原酶抑制剂,共计55例)、对照组(未服用任何药物,共计41例),采用免疫组化方法检测Beclinl、LC3、P53和Bcl-2的表达.结果 Western blot检测Beclin-1、LC3在良性前列腺增生中的表达比前列腺癌高(P＜0.001),不同Gleason分级间前列腺癌的Beclin-1、LC3表达未见差别(P＞0.05).免疫组化检测Beclin-1、LC3在服药组中的表达增高,而Bcl-2、P53降低(P＜0.05);LC3与Beclin-1呈正相关(r=0.345,P=0.001),与BCL-2呈负相关(r=-0.216,P=0.035);Beclin-1与BCL-2呈负相关(r=-0.402,P≤＜0.001).结论 自噬相关基因Beclin-1、LC3参与前列腺癌的发生发展;而服用5α-还原酶抑制剂的良性前列腺增生患者,因体内雄激素降低而诱导的自噬相关基因Beclin-1、LC3表达增强,并伴随Bcl-2和P53降低,这可能使前列腺癌变的风险降低.
目的 檢測良性前列腺增生、前列腺癌標本中自噬相關基因Beclin-1、LC3的錶達及其與凋亡基因P53、Bcl-2的關繫,併探討其意義.方法 分彆取34例前列腺癌組織和50例良性前列腺增生組織(術前均未服5α-還原酶抑製劑),採用Western blot方法檢測其Beclin-1和LC3的錶達.再將96例良性前列腺增生病理標本分為服藥組(服用5α-還原酶抑製劑,共計55例)、對照組(未服用任何藥物,共計41例),採用免疫組化方法檢測Beclinl、LC3、P53和Bcl-2的錶達.結果 Western blot檢測Beclin-1、LC3在良性前列腺增生中的錶達比前列腺癌高(P＜0.001),不同Gleason分級間前列腺癌的Beclin-1、LC3錶達未見差彆(P＞0.05).免疫組化檢測Beclin-1、LC3在服藥組中的錶達增高,而Bcl-2、P53降低(P＜0.05);LC3與Beclin-1呈正相關(r=0.345,P=0.001),與BCL-2呈負相關(r=-0.216,P=0.035);Beclin-1與BCL-2呈負相關(r=-0.402,P≤＜0.001).結論 自噬相關基因Beclin-1、LC3參與前列腺癌的髮生髮展;而服用5α-還原酶抑製劑的良性前列腺增生患者,因體內雄激素降低而誘導的自噬相關基因Beclin-1、LC3錶達增彊,併伴隨Bcl-2和P53降低,這可能使前列腺癌變的風險降低.
목적 검측량성전렬선증생、전렬선암표본중자서상관기인Beclin-1、LC3적표체급기여조망기인P53、Bcl-2적관계,병탐토기의의.방법 분별취34례전렬선암조직화50례량성전렬선증생조직(술전균미복5α-환원매억제제),채용Western blot방법검측기Beclin-1화LC3적표체.재장96례량성전렬선증생병리표본분위복약조(복용5α-환원매억제제,공계55례)、대조조(미복용임하약물,공계41례),채용면역조화방법검측Beclinl、LC3、P53화Bcl-2적표체.결과 Western blot검측Beclin-1、LC3재량성전렬선증생중적표체비전렬선암고(P＜0.001),불동Gleason분급간전렬선암적Beclin-1、LC3표체미견차별(P＞0.05).면역조화검측Beclin-1、LC3재복약조중적표체증고,이Bcl-2、P53강저(P＜0.05);LC3여Beclin-1정정상관(r=0.345,P=0.001),여BCL-2정부상관(r=-0.216,P=0.035);Beclin-1여BCL-2정부상관(r=-0.402,P≤＜0.001).결론 자서상관기인Beclin-1、LC3삼여전렬선암적발생발전;이복용5α-환원매억제제적량성전렬선증생환자,인체내웅격소강저이유도적자서상관기인Beclin-1、LC3표체증강,병반수Bcl-2화P53강저,저가능사전렬선암변적풍험강저.
Objectives To investigate the expression of autophagy-related genes Beclin-1 、LC3 in benign prostatic hyperplasia tissue and prostate cancer tissue,and to explore the correlations between it and apoptosis-related gene P53 、Bcl-2.Methods Tested using western blotting to confirm the expressions of Beclinl,LC3 in 34 cases of BPH and 50 cases of prostate cancer.Then,Immunohistochemistry were used to detect the expressions of Beclinl,LC3,P53 and Bcl-2 in 55 cases of BPH who have taken medicine and in the other 41 cases who have not.Results We found that Beclin-1,LC3 expression were higher in BPH tissues than in Prostate cancer tissues(P ＜0.001),and have no statistics difference in different Gleason stage of prostate cancer(P ＞ 0.05).Then,IHC showed that Beclin-1,LC3 expression were significantly higher in taken-medicine cases,however,Bcl-2 and were lower (P ＜ 0.05).LC3 expression was found to have positive correlation with Beclin-1 (r =0.345,P =0.001) and to have negative correlation with BCL-2 (r =-0.216,P =0.035).In addition,Beclin-1 have positive correlation with BCL-2 (r =-0.402,P ≤0.001).Conclusions Autophagy-related genes,Beclin-1 and LC3,might be involved in the genesis and development of Pca.In addition,it was higher when patients with BPH had taken 5α -reductase inhibitor due to androgen reduction.As a result,autophagy induced by that might reduce the occurrence of Pca risk.
