CAJ | 학술논문

目的探讨曲尼司特对膀胱出口部分梗阻(BOO)模型大鼠膀胱功能的保护作用及其作用机制.方法健康雌性Wistar大鼠30只随机分为空白对照组、BOO组和曲尼司特组.BOO组和曲尼司特组行尿道远端梗阻方式制备膀胱出口部分梗阻模型,空白对照组采用假手术方式处理.曲尼司特组术前1天及以后每天给予曲尼司特[400mg·(kg·d)-1]灌胃治疗,BOO组和空白对照组每天给予等体积的生理盐水灌胃,持续4周.4周后采用尿流动力仪检测膀胱容量及最大膀胱压等指标,评估膀胱的功能;测量膀胱的厚度和重量;作HE染色观察膀胱肌层增生程度;应用免疫组织化学方法检测膀胱组织转化生长因子-β1(TGF-β1)的表达.结果与空白对照组相比,BOO组尿流动力学表现最大压力(cmiH2O)与容量(mL)明显升高(62.71±9.71 vs 31.10±4.53,P＜0.05,0.72±0.13 vs 0.30±0.06,P＜0.05),术后膀胱厚度(μm)、重量(mg)增加(分别为1038.5±148.4 vs 727.1 ±74.9,P＜0.05,182.3±23.5 vs 119.0±36.0,P＜0.05),HE染色膀胱增生显著,膀胱纤维化程度未见明显增高.TGF-β1的表达增高(0.37±0.06 vs0.17±0.04,P＜0.05).与BOO组相比,曲尼司特组尿流动力学表现最大压力(cmH2O)和容量(mL)减弱(48.48±4.72 vs 62.71±9.71,P＜0.05,0.51 ±0.06 vs 0.72±0.13,P＜0.05),膀胱厚度(μm)、重量(mg)减少(分别为886.3±96.4 vs 1038.5 ±148.4,P＜0.05,155.1±16.3 vs 182.3±23.5,P＜0.05),HE染色显示膀胱增生减弱,TGF-β1的表达降低(0.29±0.06vs0.37±0.06,P＜0.05).结论曲尼司特对膀胱收缩功能有显著的保护作用,可能是通过降低TGF-β1表达改善膀胱功能.
목적 탐토곡니사특대방광출구부분경조(BOO)모형대서방광공능적보호작용급기작용궤제.방법 건강자성Wistar대서30지수궤분위공백대조조、BOO조화곡니사특조.BOO조화곡니사특조행뇨도원단경조방식제비방광출구부분경조모형,공백대조조채용가수술방식처리.곡니사특조술전1천급이후매천급여곡니사특[400mg·(kg·d)-1]관위치료,BOO조화공백대조조매천급여등체적적생리염수관위,지속4주.4주후채용뇨류동력의검측방광용량급최대방광압등지표,평고방광적공능;측량방광적후도화중량;작HE염색관찰방광기층증생정도;응용면역조직화학방법검측방광조직전화생장인자-β1(TGF-β1)적표체.결과 여공백대조조상비,BOO조뇨류동역학표현최대압력(cmiH2O)여용량(mL)명현승고(62.71±9.71 vs 31.10±4.53,P＜0.05,0.72±0.13 vs 0.30±0.06,P＜0.05),술후방광후도(μm)、중량(mg)증가(분별위1038.5±148.4 vs 727.1 ±74.9,P＜0.05,182.3±23.5 vs 119.0±36.0,P＜0.05),HE염색방광증생현저,방광섬유화정도미견명현증고.TGF-β1적표체증고(0.37±0.06 vs0.17±0.04,P＜0.05).여BOO조상비,곡니사특조뇨류동역학표현최대압력(cmH2O)화용량(mL)감약(48.48±4.72 vs 62.71±9.71,P＜0.05,0.51 ±0.06 vs 0.72±0.13,P＜0.05),방광후도(μm)、중량(mg)감소(분별위886.3±96.4 vs 1038.5 ±148.4,P＜0.05,155.1±16.3 vs 182.3±23.5,P＜0.05),HE염색현시방광증생감약,TGF-β1적표체강저(0.29±0.06vs0.37±0.06,P＜0.05).결론 곡니사특대방광수축공능유현저적보호작용,가능시통과강저TGF-β1표체개선방광공능.
Objectives To explore the intervention of tranilast on bladder function after bladder outlet obstruction in rats,and discuss mechanism of action.Methods 30 healthy 90-day-old Wistar rats were equally divided into three groups at random:sham operation group,bladder outlet obstruction (BOO) group and tranilast-treated BOO group.Tranilast was filled into the stomach one day before the operation and ever day after for 4 weeks.The same volume of water was poured down into the stomach of rats in the group of the sham operation and BOO every day.After 4 weeks rats in every group was sedated and cystometry was assayed.Urodynamic parameters were bladder capacity and maximum voiding pressure.After cystometry the bladder was weighed and the thickness of the bladder was measured.Histological changes of bladder were observed by HE.Immunohistochemical method was to detect bladder tissue expression of TGF-β 1.Results Compared with sham operation group,obstructed group showed significantly high maximal voiding pressure and capacity (62.71 ±9.71 vs 31.10 ±4.53 P ＜0.05,0.72 ±0.13 vs O.30 ± 0.06 P ＜ 0.05) resulting in bladder thickness and weight increased significantly(1038.5 ± 148.4 vs 727.1 ± 74.9 P ＜ 0.05,182.3 ± 23.5 vs 119.0 ± 36.0 P ＜ 0.05).HE staining was consistent with muscle hypertrophy in the BOO group.Bladder tissue expression of TGF-β1 rised(0.37 ± 0.06vs0.17 ± 0.04 P ＜ 0.05).Compared with BOO group,tranilast-treated BOO group had the low maximal voiding pressure and capacity (48.48 ± 4.72 vs 62.71 ± 9.71 P ＜ 0.05,0.51 ± 0.06 vs 0.72 ± 0.13 P ＜ 0.05)with the bladder thickness and weight decreasing(886.3 ± 96.4 vs 1038.5 ± 148.4 P ＜ 0.05,155.1 ± 16.3 vs 182.3 ± 23.5 P ＜ 0.05).HE staining showed an abvious improvement of muscle hypertrophy in treated group.Bladder tissue expression of TGF-β1 declined (0.29 ± 0.06 vs0.37± 0.06 P ＜ 0.05).Conclusions Tranilast has obvious protective effect of bladder contraction function,red-ucing the TGF-beta 1 expression might be one of the evidence to improve the bladder function.