CAJ | 학술논문

目的研究Akt抑制剂鱼藤素对膀胱癌T-24细胞株的生长抑制作用,并初步探讨其可能的作用机制.方法用MTT法检测鱼藤素对T-24细胞生长的抑制率;流式细胞术检测细胞周期的改变;DNA片段化检测鱼藤素对T-24细胞凋亡的影响;RT-PCR检测鱼藤素对MDM2和GSK3β mRNA表达的影响;Western blotting检测MDM2、GSK3β蛋白表达水平的变化.结果鱼藤素显著抑制T-24细胞的增殖,使细胞周期主要阻滞在G0/G1期,并可诱导T-24细胞发生凋亡.RT-PCR结果显示,鱼藤素处理T-24细胞后MDM2 mRNA的表达水平下降,而GSK3 β mRNA的表达水平增加;Western blotting检测发现鱼藤素下调MDM2蛋白的表达,而上调GSK3β蛋白的表达.结论 Akt抑制剂鱼藤素能抑制T-24细胞的增殖并诱导其凋亡,其机制可能与影响Akt通路下游信号分子MDM2和GSK3β的表达相关.
목적 연구Akt억제제어등소대방광암T-24세포주적생장억제작용,병초보탐토기가능적작용궤제.방법 용MTT법검측어등소대T-24세포생장적억제솔;류식세포술검측세포주기적개변;DNA편단화검측어등소대T-24세포조망적영향;RT-PCR검측어등소대MDM2화GSK3β mRNA표체적영향;Western blotting검측MDM2、GSK3β단백표체수평적변화.결과 어등소현저억제T-24세포적증식,사세포주기주요조체재G0/G1기,병가유도T-24세포발생조망.RT-PCR결과현시,어등소처리T-24세포후MDM2 mRNA적표체수평하강,이GSK3 β mRNA적표체수평증가;Western blotting검측발현어등소하조MDM2단백적표체,이상조GSK3β단백적표체.결론 Akt억제제어등소능억제T-24세포적증식병유도기조망,기궤제가능여영향Akt통로하유신호분자MDM2화GSK3β적표체상관.
Objectives To study the effects of Akt inhibitor deguelin on the growth of human bladder cancer cell lines T-24,and to elucidate the possible mechanism of such effects.Methods MTT method was used to determine growth-inhibitory effect of deguelin on human bladder cancer T-24 cells.The cell cycle was detected by flow cytometry (FCM).Apoptosis induced by deguelin in T-24 cells was determined using DNA fragmentation assay.The expression of MDM2 and GSK3β in mRNA and protein levels were detected by RT-PCR and Western blotting respectively.Results The deguelin singnificantly inhibited cell proliferation,blocked the cell cycle in the G0/G1 phase and induced apoptosis in T-24 cells.The expression levels of MDM2 mRNA and protein were down-regulated,while the expression levels of GSK3 β mRNA and proteins were up-regulated when T-24 cell were treated by deguelin.Conclusions Deguelin can inhibit the proliferation and induce the apoptosis in T-24 human bladder cancer cells.These effects may be related with its function in down-regulating MDM2 and up-regulating GSK3 β,both of them are down-stream signal meleculars in the AKt pathway.