国际泌尿系统杂志
國際泌尿繫統雜誌
국제비뇨계통잡지
INTERNATIONAL JOURNAL OF UROLOGY AND NEPHROLOGY
2014年
5期
641-646
,共6页
朱奇%祝宇%徐云泽%陈东宁%徐烈雨%张翀宇%赵菊平%吴瑜璇%沈周俊
硃奇%祝宇%徐雲澤%陳東寧%徐烈雨%張翀宇%趙菊平%吳瑜璇%瀋週俊
주기%축우%서운택%진동저%서렬우%장충우%조국평%오유선%침주준
嗜铬细胞瘤%热休克蛋白质类%肿瘤移植
嗜鉻細胞瘤%熱休剋蛋白質類%腫瘤移植
기락세포류%열휴극단백질류%종류이식
Pheochromocytoma%Heat-Shock Proteins%Neoplasm Transplantation
目的 本研究通过观察热休克蛋白90(HSP90)抑制剂17-丙烯胺-17～去甲氧格尔德霉素(17-AAG)对大鼠嗜铬细胞瘤裸鼠移植瘤生长的影响,探讨17-AAG对大鼠嗜铬细胞瘤肿瘤生长和血管生成的抑制作用.方法 用大鼠嗜铬细胞瘤细胞PC12接种于裸鼠皮下,建立移植瘤模型,15d后将荷瘤裸鼠随机分为2组,每组12只,分别为实验组(腹腔注射17-AAG 40mg/kg)和对照组(腹腔注射生理盐水10mL/kg),用药3周,第4周后测量裸鼠移植瘤体积和重量,采用免疫组化方法检测肿瘤组织中HSP90、信号转导和转录活化因子3(STAT3)、表皮生长因子受体-2(ERBB2)的表达,采用western blotting法检测肿瘤组织中HSP90、STAT3、ERBB2的表达.结果 第4周时,对照组的移植瘤体积为5070.13±630.42mm3,重量为7.94±1.20g;实验组的移植瘤体积为2218.80±336.29mm3,重量为3.29±0.57g,实验组抑瘤率为58.6％,两组移植瘤体积和重量存在显著差异(P＜0.05).实验组肿瘤组织的HSP90、STAT3、ERBB2表达明显低于对照组(P＜0.05).结论 17-AAG对大鼠嗜铬细胞瘤裸鼠移植瘤有明显抑制作用,并可降低肿瘤组织中HSP90和STAT3、ERBB2的表达.
目的 本研究通過觀察熱休剋蛋白90(HSP90)抑製劑17-丙烯胺-17～去甲氧格爾德黴素(17-AAG)對大鼠嗜鉻細胞瘤裸鼠移植瘤生長的影響,探討17-AAG對大鼠嗜鉻細胞瘤腫瘤生長和血管生成的抑製作用.方法 用大鼠嗜鉻細胞瘤細胞PC12接種于裸鼠皮下,建立移植瘤模型,15d後將荷瘤裸鼠隨機分為2組,每組12隻,分彆為實驗組(腹腔註射17-AAG 40mg/kg)和對照組(腹腔註射生理鹽水10mL/kg),用藥3週,第4週後測量裸鼠移植瘤體積和重量,採用免疫組化方法檢測腫瘤組織中HSP90、信號轉導和轉錄活化因子3(STAT3)、錶皮生長因子受體-2(ERBB2)的錶達,採用western blotting法檢測腫瘤組織中HSP90、STAT3、ERBB2的錶達.結果 第4週時,對照組的移植瘤體積為5070.13±630.42mm3,重量為7.94±1.20g;實驗組的移植瘤體積為2218.80±336.29mm3,重量為3.29±0.57g,實驗組抑瘤率為58.6％,兩組移植瘤體積和重量存在顯著差異(P＜0.05).實驗組腫瘤組織的HSP90、STAT3、ERBB2錶達明顯低于對照組(P＜0.05).結論 17-AAG對大鼠嗜鉻細胞瘤裸鼠移植瘤有明顯抑製作用,併可降低腫瘤組織中HSP90和STAT3、ERBB2的錶達.
목적 본연구통과관찰열휴극단백90(HSP90)억제제17-병희알-17～거갑양격이덕매소(17-AAG)대대서기락세포류라서이식류생장적영향,탐토17-AAG대대서기락세포류종류생장화혈관생성적억제작용.방법 용대서기락세포류세포PC12접충우라서피하,건립이식류모형,15d후장하류라서수궤분위2조,매조12지,분별위실험조(복강주사17-AAG 40mg/kg)화대조조(복강주사생리염수10mL/kg),용약3주,제4주후측량라서이식류체적화중량,채용면역조화방법검측종류조직중HSP90、신호전도화전록활화인자3(STAT3)、표피생장인자수체-2(ERBB2)적표체,채용western blotting법검측종류조직중HSP90、STAT3、ERBB2적표체.결과 제4주시,대조조적이식류체적위5070.13±630.42mm3,중량위7.94±1.20g;실험조적이식류체적위2218.80±336.29mm3,중량위3.29±0.57g,실험조억류솔위58.6％,량조이식류체적화중량존재현저차이(P＜0.05).실험조종류조직적HSP90、STAT3、ERBB2표체명현저우대조조(P＜0.05).결론 17-AAG대대서기락세포류라서이식류유명현억제작용,병가강저종류조직중HSP90화STAT3、ERBB2적표체.
Objectives In order to investigate the effect of heat shock protein 90(HSP90) inhibitor 17-allylamino-17-demethoxygeldanamycin(17-AAG) in the implanted malignant pheochromocytoma nude mice and find the inhibitory mechanism of 17-AAG on the tumor growth and angiopoiesis of malignant pheochromocytoma.Methods We created the malignant pheochromocytoma xenograft model by implanting PC12 cells in the left flank of nude mice.After fifteen days,nude mice were randomly divided into two groups:the experiment group (receiving 17-AAG at dose of 40 mg/kg) and the control group (treated with NS at dose of 10 ml/kg).After three weeks' treatment,the tumors were obtained,then we measured the tumor volumes and weights in the fourth week.During this time,we observed the change of tumor volumes.The expressions of HSP90 and its client proteins signal transducers and activators of transcription3 (STAT3) 、human Epidermal Growth Factor Receptor.Results 2 (ERBB2) were detected by immunohistochemical staining and western blotting.In the fourth week,the tumor size in the experiment group is 2218.80 ± 336.29mm2,the weight is 7.94 ± 1.20g,while that in the control group is 5070.13 ± 630.42mm2,the weight is 3.29 ± 0.57g.A significant decrease of the size and weight of xenografts in the experiment group was observed as compared to those in the control group.The inhibitory rate of 17-AAG is 58.6％.The growth of tumor in the experiment group is slower than that in the control group,which has a statistical significance.The expressions of HSP90 、STAT3 and ERBB2 of tumor issues in the experiment group are each significantly lower than those in the control group(P ＜ 0.05).Conclusions 17-AAG has a significant effect on the transplanted pheochromocytoma in nude mice and suppresses the expression of HSP90 and STAT3 、ERBB2 in the tumor tissues.
