国际泌尿系统杂志
國際泌尿繫統雜誌
국제비뇨계통잡지
INTERNATIONAL JOURNAL OF UROLOGY AND NEPHROLOGY
2014年
5期
654-659
,共6页
糖尿病肾病%Von Willebrand病%大鼠
糖尿病腎病%Von Willebrand病%大鼠
당뇨병신병%Von Willebrand병%대서
Diabetic Nephropathies%Von Willebrand Disease%Rats
目的 观察丹红注射液干预前后糖尿病大鼠血清血管性假血友病因子(vWF)水平及肾组织中vWF的表达变化,探讨糖尿病肾病发病机制及丹红的干预效果.方法 36只雄性SD大鼠随机分为3组:正常对照组10只、糖尿病组14只、丹红干预组12只.除正常对照组外,其余各组均按55mg/kg剂量一次性腹腔注射1％链脲佐菌素(STZ)建立糖尿病大鼠模型.72h后采尾静脉血测血糖,以血糖值＞16.7mmol/L作为糖尿病大鼠模型建立的标准.造模成功后,丹红干预组按2mL/kg.d剂量腹腔注射丹红注射液.干预为期8周.于第8周末,收集各组大鼠血液、尿液及肾组织标本.然后检测血糖(BG)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、血肌酐(Scr)、尿素氮(BUN).放射免疫法检测24h尿白蛋白定量.酶联免疫分析法(ELISA)测定各组大鼠血清vWF的水平.肾组织进行HE染色在光镜观察肾脏形态学变化.用免疫组化的方法检测各组大鼠肾脏vWF的表达情况.应用实时荧光定量PCR技术检测肾组织vWF的表达情况.结果 糖尿病组大鼠血糖、胆固醇、甘油三酯、低密度脂蛋白胆固醇、血肌酐、尿素氮、24h尿白蛋白定量、肾脏肥大指数、血vWF水平明显高于正常对照组(P＜0.01),糖尿病大鼠肾脏出现病理改变,糖尿病大鼠肾组织vWF的表达较正常对照组大鼠明显增加;应用丹红干预8周后,干预组大鼠胆固醇、甘油三酯、低密度脂蛋白胆固醇、血肌酐、尿素氮、24h尿白蛋白定量、肾脏肥大指数、血清vWF水平较糖尿病组明显减低(P＜0.05),肾组织病理损伤减轻,肾脏vWF表达较糖尿病组明显降低.结论 糖尿病大鼠血清vWF水平及肾组织vWF表达增加,提示vWF与糖尿病肾病关系密切,可能参与了糖尿病肾病的发生、发展;而丹红能通过调节血脂、降低尿白蛋白、降低vWF的表达来发挥肾脏保护作用.
目的 觀察丹紅註射液榦預前後糖尿病大鼠血清血管性假血友病因子(vWF)水平及腎組織中vWF的錶達變化,探討糖尿病腎病髮病機製及丹紅的榦預效果.方法 36隻雄性SD大鼠隨機分為3組:正常對照組10隻、糖尿病組14隻、丹紅榦預組12隻.除正常對照組外,其餘各組均按55mg/kg劑量一次性腹腔註射1％鏈脲佐菌素(STZ)建立糖尿病大鼠模型.72h後採尾靜脈血測血糖,以血糖值＞16.7mmol/L作為糖尿病大鼠模型建立的標準.造模成功後,丹紅榦預組按2mL/kg.d劑量腹腔註射丹紅註射液.榦預為期8週.于第8週末,收集各組大鼠血液、尿液及腎組織標本.然後檢測血糖(BG)、總膽固醇(TC)、甘油三酯(TG)、低密度脂蛋白膽固醇(LDL-C)、血肌酐(Scr)、尿素氮(BUN).放射免疫法檢測24h尿白蛋白定量.酶聯免疫分析法(ELISA)測定各組大鼠血清vWF的水平.腎組織進行HE染色在光鏡觀察腎髒形態學變化.用免疫組化的方法檢測各組大鼠腎髒vWF的錶達情況.應用實時熒光定量PCR技術檢測腎組織vWF的錶達情況.結果 糖尿病組大鼠血糖、膽固醇、甘油三酯、低密度脂蛋白膽固醇、血肌酐、尿素氮、24h尿白蛋白定量、腎髒肥大指數、血vWF水平明顯高于正常對照組(P＜0.01),糖尿病大鼠腎髒齣現病理改變,糖尿病大鼠腎組織vWF的錶達較正常對照組大鼠明顯增加;應用丹紅榦預8週後,榦預組大鼠膽固醇、甘油三酯、低密度脂蛋白膽固醇、血肌酐、尿素氮、24h尿白蛋白定量、腎髒肥大指數、血清vWF水平較糖尿病組明顯減低(P＜0.05),腎組織病理損傷減輕,腎髒vWF錶達較糖尿病組明顯降低.結論 糖尿病大鼠血清vWF水平及腎組織vWF錶達增加,提示vWF與糖尿病腎病關繫密切,可能參與瞭糖尿病腎病的髮生、髮展;而丹紅能通過調節血脂、降低尿白蛋白、降低vWF的錶達來髮揮腎髒保護作用.
목적 관찰단홍주사액간예전후당뇨병대서혈청혈관성가혈우병인자(vWF)수평급신조직중vWF적표체변화,탐토당뇨병신병발병궤제급단홍적간예효과.방법 36지웅성SD대서수궤분위3조:정상대조조10지、당뇨병조14지、단홍간예조12지.제정상대조조외,기여각조균안55mg/kg제량일차성복강주사1％련뇨좌균소(STZ)건립당뇨병대서모형.72h후채미정맥혈측혈당,이혈당치＞16.7mmol/L작위당뇨병대서모형건립적표준.조모성공후,단홍간예조안2mL/kg.d제량복강주사단홍주사액.간예위기8주.우제8주말,수집각조대서혈액、뇨액급신조직표본.연후검측혈당(BG)、총담고순(TC)、감유삼지(TG)、저밀도지단백담고순(LDL-C)、혈기항(Scr)、뇨소담(BUN).방사면역법검측24h뇨백단백정량.매련면역분석법(ELISA)측정각조대서혈청vWF적수평.신조직진행HE염색재광경관찰신장형태학변화.용면역조화적방법검측각조대서신장vWF적표체정황.응용실시형광정량PCR기술검측신조직vWF적표체정황.결과 당뇨병조대서혈당、담고순、감유삼지、저밀도지단백담고순、혈기항、뇨소담、24h뇨백단백정량、신장비대지수、혈vWF수평명현고우정상대조조(P＜0.01),당뇨병대서신장출현병리개변,당뇨병대서신조직vWF적표체교정상대조조대서명현증가;응용단홍간예8주후,간예조대서담고순、감유삼지、저밀도지단백담고순、혈기항、뇨소담、24h뇨백단백정량、신장비대지수、혈청vWF수평교당뇨병조명현감저(P＜0.05),신조직병리손상감경,신장vWF표체교당뇨병조명현강저.결론 당뇨병대서혈청vWF수평급신조직vWF표체증가,제시vWF여당뇨병신병관계밀절,가능삼여료당뇨병신병적발생、발전;이단홍능통과조절혈지、강저뇨백단백、강저vWF적표체래발휘신장보호작용.
Objectives To observe the level of serum yon Willebrand factor(vWF) and expression of vWF in Kidney of Diabetic Rats to study the change of vWF before and after the interventionof Danhong.The pathogenesis of diabetic nephropathy and renoprotective effects of Danhong are specially studied.Methods 36 SD rats were randomly divided into three groups as follows:10 normol rats,14 diabetic rats,11 Danhong intervention ratsand 11 Metformin intervention rats. (Two died in diabetic group,one died in Danhong intervention group) . Except the normal control group,all the rats in the other groups were established an animal model of diabetes by a single intraperitoneal injection of streptozotocin (55mg/kg).After72 hours,get blood from caudal vein to test blood sugar,those higher than 16.7mmol/L regard as succeed in diabetic models.Then Danhong (2ml/kg.d) was given by intraperitoneal injection to Danhong intervention group.The same amount of water was given by intraperitoneal injection to normal control group and diabetic group.The experiment lasted for eight weeks.At the end of eight weeks,blood,urine and renal tissue samples were collected in four groups of rats.Then blood sugar,cholesterol (TC),triglycerides (TG),Low density lipoprotein cholesterol (LDL-C),Serum creatinine (Scr),Urea nitrogen (BUN) were tested.24h Urinary albuminwere measured by radioimmunoassay.Serum vWF was determined by Enzyme-linked immunoassay (ELISA).We used HE staining to observe the renal pathological changes under optical microscope. Expression of vWF in renal tissue was measured by immunohistochemistry.Renal vWFmRNA expression was measured by Real-time fluorescent quantitative PCR.Results After eight weeks,Compared with normal control group,blood sugar,TC,TG,LDL-C,Scr,BUN,Kidney hypertrophy index,24h Urinary albumin,Serum vWF were all inereased significantlyin diabetic group(P ＜0.01).Kidneys of Diabetic rats have obvious pathological changes.The expression of vWF in Diabetic rat kidneywas obviously increased compared with normal control group.After treated by Danhong,TC,TG,LDL-C,Scr,BUN,Kidney hypertrophy index,24h Urinary albumin,Serum vWFwerereduced significantly (P ＜ 0.05) and there was a mitigated pathomorphology change in renal tissues.The expression of vWFin rat kidney was decreased significantly.Conclusions The Serum vWF level and the expression of vWFin diabetie rats were increased significantly,which may be involved in the occurence and development of diabetic nephropathy.Danhong have renoprotective effect on diabetic nephropathy byregulating thelevels of serum lipids,reducing the level of albuminuria and suppressing vWF expression.
