CAJ | 학술논문

目的研究RAS样家族成员11B(RASL11B)在肾透明细胞癌中的表达特征及意义.方法收集36例肾透明细胞癌患者的癌组织和对应癌旁组织,采用实时荧光定量PCR方法,检测RASL11BmRNA的表达差异,并分析RASL11B mRNA的表达水平与临床病理参数的关系;分别采用Western-blot和免疫组化的方法,确定RASL11B蛋白在癌组织和癌旁组织的表达差异及定位.结果实时荧光定量PCR结果显示,肾透明细胞癌组织和癌旁组织均表达RASL11B mRNA,与癌旁组织(1.93±0.43)比较,其表达量在癌组织(0.28±0.08)中显著降低,两组比较有显著性差异(P＜0.001);RASL11B mRNA的表达水平下调与肿瘤直径大小、T分期、组织学分级、临床分级及有无大血管浸润呈显著负相关性;而与年龄和性别无显著相关性.Western-blot结果显示,癌组织和癌旁组织均表达RASL11B蛋白,癌组织表达量明显低于癌旁组织,Western-blot条带半定量分析结果分别为0.98±0.06、1.43±0.15,有显著性差异(P＜0.05);免疫组化染色结果显示RASL11B蛋白主要定位于正常肾组织肾小管上皮细胞胞浆;癌组织和癌旁组织的免疫组化染色评分结果分别为0.81±0.16、3.00±0.31,两组比较有显著性差异(P＜0.001).结论 RASL11B在肾透明细胞癌组织中的表达较癌旁组织显著降低,提示该基因的低表达在肾透明细胞癌的发生发展过程中具有一定的促进作用.
목적 연구RAS양가족성원11B(RASL11B)재신투명세포암중적표체특정급의의.방법 수집36례신투명세포암환자적암조직화대응암방조직,채용실시형광정량PCR방법,검측RASL11BmRNA적표체차이,병분석RASL11B mRNA적표체수평여림상병리삼수적관계;분별채용Western-blot화면역조화적방법,학정RASL11B단백재암조직화암방조직적표체차이급정위.결과 실시형광정량PCR결과현시,신투명세포암조직화암방조직균표체RASL11B mRNA,여암방조직(1.93±0.43)비교,기표체량재암조직(0.28±0.08)중현저강저,량조비교유현저성차이(P＜0.001);RASL11B mRNA적표체수평하조여종류직경대소、T분기、조직학분급、림상분급급유무대혈관침윤정현저부상관성;이여년령화성별무현저상관성.Western-blot결과현시,암조직화암방조직균표체RASL11B단백,암조직표체량명현저우암방조직,Western-blot조대반정량분석결과분별위0.98±0.06、1.43±0.15,유현저성차이(P＜0.05);면역조화염색결과현시RASL11B단백주요정위우정상신조직신소관상피세포포장;암조직화암방조직적면역조화염색평분결과분별위0.81±0.16、3.00±0.31,량조비교유현저성차이(P＜0.001).결론 RASL11B재신투명세포암조직중적표체교암방조직현저강저,제시해기인적저표체재신투명세포암적발생발전과정중구유일정적촉진작용.
Objectives To investigate the expression and clinical values of RAS-like,family 11,member B (RASL11 B) in clear cell renal cell carcinoma (ccRCC).Methods Tumor tissues and adjacent non-cancerous kidney tissues were collected from 78 ccRCC undergoing nephrectomy.The expression level of RASL11B mRNA in ccRCC was measured by Quantitative Real-Time PCR.Western blot and immunohistochemistry were used to determine the expression and localizaiton of RASL11B in ccRCC and corresponding normal tissues.Results The expression level of RASL11B mRNA was significantly decreased in ccRCC tumor tissues compared with adjacent non -cancerous kidney tissues (P ＜ 0.001) ; Low RASL11 B mRNA was significantly associated with tumor clinicopathological parameters,including tumor diameter,pT stage,Fuhrman tumor grade,TNM stage grouping and macrovascular infiltration(MAVI).RASL11 B protein signals were mainly localized in the cytoplasm of normal renal tubule epithelial cells.Lower RASL11B protein expression was consistent to the trend by PCR.Conclusions The expression of RASL11B was significantly decreased in the clear cell renal cell carcinoma tissues compared to the adjacent non-cancerous tissues,which suggests that RASL11B play an important role in ccRCC.