国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2012年
6期
466-469
,共4页
付爱丽%曹奇志%梅延辉%曹晏宁%薛江楠%张晓姝
付愛麗%曹奇誌%梅延輝%曹晏寧%薛江楠%張曉姝
부애려%조기지%매연휘%조안저%설강남%장효주
抑制性受体%LAIR-1%宫颈癌%子宫内膜癌%免疫逃逸
抑製性受體%LAIR-1%宮頸癌%子宮內膜癌%免疫逃逸
억제성수체%LAIR-1%궁경암%자궁내막암%면역도일
Inhibitory receptors%LAIR-1%Cervical cancer%Endometrial carcinoma%Immune escape
目的 研究白细胞相关免疫球蛋白样受体(LAIR-1)在宫颈癌和子宫内膜癌患者外周血T细胞的表达情况,并与子宫肌瘤、子宫癌前病变患者相比较,探讨LAIR-1表达与肿瘤免疫的相关性.方法 密度梯度离心法分离外周血单个核细胞,流式细胞术检测LAIR-1在196例肿瘤患者外周血T细胞的表达水平.结果 流式细胞术检测结果显示,宫颈癌和子宫内膜癌患者外周血CD3+CD4+T细胞LAIR-1阳性率均高于子宫肌瘤(P＜0.001;P＜0.05)和癌前病变患者(P ＜0.001;P ＜0.01).平均荧光强度分析结果显示,宫颈癌患者外周血CD3+CD4+T细胞LAIR-1表达水平显著高于癌前病变患者(P＜0.01),CD3+CD8+T细胞LAIR-1表达水平显著高于子宫肌瘤患者(P＜0.05)和癌前病变患者(P＜0.001).子宫内膜癌患者外周血CD3+CD4+T细胞LAIR-1表达水平明显高于子宫肌瘤患者(P＜0.05)和癌前病变患者(P＜0.01),CD3+CD8+T细胞LAIR-1表达水平高于癌前病变患者(P＜0.05).结论 LAIR-1在宫颈癌和子宫内膜癌患者外周血T细胞表达上调,提示LAIR-1可能与肿瘤免疫逃逸存在相关性.
目的 研究白細胞相關免疫毬蛋白樣受體(LAIR-1)在宮頸癌和子宮內膜癌患者外週血T細胞的錶達情況,併與子宮肌瘤、子宮癌前病變患者相比較,探討LAIR-1錶達與腫瘤免疫的相關性.方法 密度梯度離心法分離外週血單箇覈細胞,流式細胞術檢測LAIR-1在196例腫瘤患者外週血T細胞的錶達水平.結果 流式細胞術檢測結果顯示,宮頸癌和子宮內膜癌患者外週血CD3+CD4+T細胞LAIR-1暘性率均高于子宮肌瘤(P＜0.001;P＜0.05)和癌前病變患者(P ＜0.001;P ＜0.01).平均熒光彊度分析結果顯示,宮頸癌患者外週血CD3+CD4+T細胞LAIR-1錶達水平顯著高于癌前病變患者(P＜0.01),CD3+CD8+T細胞LAIR-1錶達水平顯著高于子宮肌瘤患者(P＜0.05)和癌前病變患者(P＜0.001).子宮內膜癌患者外週血CD3+CD4+T細胞LAIR-1錶達水平明顯高于子宮肌瘤患者(P＜0.05)和癌前病變患者(P＜0.01),CD3+CD8+T細胞LAIR-1錶達水平高于癌前病變患者(P＜0.05).結論 LAIR-1在宮頸癌和子宮內膜癌患者外週血T細胞錶達上調,提示LAIR-1可能與腫瘤免疫逃逸存在相關性.
목적 연구백세포상관면역구단백양수체(LAIR-1)재궁경암화자궁내막암환자외주혈T세포적표체정황,병여자궁기류、자궁암전병변환자상비교,탐토LAIR-1표체여종류면역적상관성.방법 밀도제도리심법분리외주혈단개핵세포,류식세포술검측LAIR-1재196례종류환자외주혈T세포적표체수평.결과 류식세포술검측결과현시,궁경암화자궁내막암환자외주혈CD3+CD4+T세포LAIR-1양성솔균고우자궁기류(P＜0.001;P＜0.05)화암전병변환자(P ＜0.001;P ＜0.01).평균형광강도분석결과현시,궁경암환자외주혈CD3+CD4+T세포LAIR-1표체수평현저고우암전병변환자(P＜0.01),CD3+CD8+T세포LAIR-1표체수평현저고우자궁기류환자(P＜0.05)화암전병변환자(P＜0.001).자궁내막암환자외주혈CD3+CD4+T세포LAIR-1표체수평명현고우자궁기류환자(P＜0.05)화암전병변환자(P＜0.01),CD3+CD8+T세포LAIR-1표체수평고우암전병변환자(P＜0.05).결론 LAIR-1재궁경암화자궁내막암환자외주혈T세포표체상조,제시LAIR-1가능여종류면역도일존재상관성.
Objective To investigate the expression of leukocyte-associated immunoglobulin-like receptor-1 (LAIR)-1 in T cells from the peripheral blood of patients with cervical cancer and endometrial carcinoma.Methods Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of 196 tumor patients.Flow cytometry was used to analyze the expression of LAIR-1 in T cells Results The number of CD3+CD4+T cells expressing LAIR-1 in patients with cervical cancer and endometrial carcinoma was greater than that in patients with hysteromyoma(P ＜ 0.001,P ＜ 0.05) and precancerous lesion (P ＜ 0.001,P ＜ 0.01) respectively.Mean fluorescence intensity (MFI) analysis showed that the expression of LAIR-1 in CD3+CD4+T cells in patients with cervical cancer was higher than that in precancerous lesion patients(P ＜0.01),the expression of LAIR-1 in CD3+CD8+T cells was higher than that in patients with hysteromyoma(P ＜ 0.05)and precancerous lesion(P ＜ 0.001).The expression of LAIR-1 in CD3+CD4+T cells in patients with endometrial carcinoma was higher than that in patients with hysteromyoma(P ＜ 0.05) and precancerous lesion (P ＜ 0.01),the expression of LAIR-1 in CD3+CD8+T cells was higher than that in patients with precancerous lesion (P ＜ 0.05).Conclusions The expression of LAIR-1 in T cells of patients with cervical cancer and endometrial carcinoma was up-regulated.
