CAJ | 학술논문

目的观察电针刺激足三里穴位对实验性自身免疫性脑脊髓炎(EAE)大鼠体内分泌的细胞因子的影响.方法将免疫大鼠随机分为四组:用完全弗氏佐剂免疫组(CFA组);用MBPB68-86B多肽与完全弗氏佐剂免疫组(EAE组);用MBPB68-86B多肽免疫后的大鼠电针刺激足三里穴位组和用MBPB68-86B多肽免疫后的大鼠电针刺激非穴位组.分别于免疫后第7天、14天和21天采用ELISA方法检测大鼠血浆中IFN-γ、IL-4、IL-17、IL-6和TGF-β细胞因子水平.结果 EA组与EAE组大鼠血浆中IL-4水平有所升高(第14天P=0.027,第21天P=0.36);IFN-γ表达量在第14天EA组明显低于EAE组(P=0.00021);IL-6在三个时间点EA组均明显低于EAE组(P=0.0078,P=0.00047,P=0.00093);IL-17免疫21天,EA组明显低于EAE组(P=0.0012);TGF-β在第7天和第14天EA组明显高于EAE组(P=0.020,P=0.041)结论电针刺激足三里穴位对EAE大鼠的治疗作用,通过降低炎症细胞因子的产生,促进抑炎细胞因子的产生而发挥作用.
목적 관찰전침자격족삼리혈위대실험성자신면역성뇌척수염(EAE)대서체내분비적세포인자적영향.방법 장면역대서수궤분위사조:용완전불씨좌제면역조(CFA조);용MBPB68-86B다태여완전불씨좌제면역조(EAE조);용MBPB68-86B다태면역후적대서전침자격족삼리혈위조화용MBPB68-86B다태면역후적대서전침자격비혈위조.분별우면역후제7천、14천화21천채용ELISA방법검측대서혈장중IFN-γ、IL-4、IL-17、IL-6화TGF-β세포인자수평.결과 EA조여EAE조대서혈장중IL-4수평유소승고(제14천P=0.027,제21천P=0.36);IFN-γ표체량재제14천EA조명현저우EAE조(P=0.00021);IL-6재삼개시간점EA조균명현저우EAE조(P=0.0078,P=0.00047,P=0.00093);IL-17면역21천,EA조명현저우EAE조(P=0.0012);TGF-β재제7천화제14천EA조명현고우EAE조(P=0.020,P=0.041)결론 전침자격족삼리혈위대EAE대서적치료작용,통과강저염증세포인자적산생,촉진억염세포인자적산생이발휘작용.
Objective The purpose of the study was to examine the effects of electroacupuncture (EA) on cytokine in rats with experimental autoimmune encephalomyelitis (EAE).Methods Animals after immunization were divided into four groups:complete Freund ' s adjuvant (CFA group),EAE group,electroa-cupuncture stimulating on Zusanli ST36 acupoints group (EA group) and non-acupoint group (NA group).The concentrations in plasma were determined using ELISA assay.Results The levels of IL-4 were higher in the EA group compared that in the EAE group(P14d =0.027,P21d =0.36) ; lFN-γ in 14 day from EA group was lower compared with EAE group (P =0.00021) ; the IL-6 levels was significantly reduced on day 7,day 14 and 21 in the EA group compared to the EAE group(P =0.0078,P =0.00047,P =0.00093) ; the levels of IL-17 in 21 day was lower in EA group compared with EAE group(P =0.0012) ; and TGF-β was higher in EA group compared with EAE group in 7 and 14 day(P =0.020,P =0.041).Conclusion Successive electroacupunc ture stimuiation on Zusanli ST36 acupoints of the rats with EAE inhibites proinflammatory cytokines and inhibits suppression of inflammatory cytokines.