国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2013年
1期
54-56
,共3页
金茜%杜博%孙文英%李兴库
金茜%杜博%孫文英%李興庫
금천%두박%손문영%리흥고
肾移植%抗内皮细胞抗体%非HLA抗体%慢性排斥反应
腎移植%抗內皮細胞抗體%非HLA抗體%慢性排斥反應
신이식%항내피세포항체%비HLA항체%만성배척반응
Renal transplantation%Anti-endothelial cell antibody%Non-HLA antibodies%Chronic rejection reaction
目的 对临床发生慢性排斥反应的肾移植患者及移植术前透析患者的血清中的抗内皮细胞抗体(非HLA抗体)的含量进行定量检测,探讨在肾移植慢性排斥中非HLA抗体的作用.方法 采用免疫荧光法对252份血清标本进行抗内皮细胞抗体的检测,包括移植前72例,移植后100例,正常对照组80例.结果 移植前透析患者抗内皮细胞抗体的阳性率为38.89％ (28/72),其中强阳性占13.89％ (10/72),弱阳性占25.00％ (18/72)；移植后慢性排斥期患者抗内皮细胞抗体的阳性率为70.00％ (70/100),其中强阳性占32.00％.(32/100),弱阳性占38.00％ (38/100)；正常对照组抗内皮细胞抗体的阳性率为30.00％ (24/80),其中强阳性为10.00％ (8/80),弱阳性为20.00％ (16/80).移植前与正常对照组抗内皮细胞抗体含量无统计学意义(t=1.0105,P=0.1566),移植前后抗内皮细胞抗体含量差异有统计学意义(t =4.3311,P=0.0000),移植后与正常对照组抗内皮细胞抗体含量差异也具有统计学意义(t =5.5572,P=0.0000).结论 抗内皮细胞抗体作为非HLA抗体是肾移植慢性排斥反应的影响因素之一.
目的 對臨床髮生慢性排斥反應的腎移植患者及移植術前透析患者的血清中的抗內皮細胞抗體(非HLA抗體)的含量進行定量檢測,探討在腎移植慢性排斥中非HLA抗體的作用.方法 採用免疫熒光法對252份血清標本進行抗內皮細胞抗體的檢測,包括移植前72例,移植後100例,正常對照組80例.結果 移植前透析患者抗內皮細胞抗體的暘性率為38.89％ (28/72),其中彊暘性佔13.89％ (10/72),弱暘性佔25.00％ (18/72)；移植後慢性排斥期患者抗內皮細胞抗體的暘性率為70.00％ (70/100),其中彊暘性佔32.00％.(32/100),弱暘性佔38.00％ (38/100)；正常對照組抗內皮細胞抗體的暘性率為30.00％ (24/80),其中彊暘性為10.00％ (8/80),弱暘性為20.00％ (16/80).移植前與正常對照組抗內皮細胞抗體含量無統計學意義(t=1.0105,P=0.1566),移植前後抗內皮細胞抗體含量差異有統計學意義(t =4.3311,P=0.0000),移植後與正常對照組抗內皮細胞抗體含量差異也具有統計學意義(t =5.5572,P=0.0000).結論 抗內皮細胞抗體作為非HLA抗體是腎移植慢性排斥反應的影響因素之一.
목적 대림상발생만성배척반응적신이식환자급이식술전투석환자적혈청중적항내피세포항체(비HLA항체)적함량진행정량검측,탐토재신이식만성배척중비HLA항체적작용.방법 채용면역형광법대252빈혈청표본진행항내피세포항체적검측,포괄이식전72례,이식후100례,정상대조조80례.결과 이식전투석환자항내피세포항체적양성솔위38.89％ (28/72),기중강양성점13.89％ (10/72),약양성점25.00％ (18/72)；이식후만성배척기환자항내피세포항체적양성솔위70.00％ (70/100),기중강양성점32.00％.(32/100),약양성점38.00％ (38/100)；정상대조조항내피세포항체적양성솔위30.00％ (24/80),기중강양성위10.00％ (8/80),약양성위20.00％ (16/80).이식전여정상대조조항내피세포항체함량무통계학의의(t=1.0105,P=0.1566),이식전후항내피세포항체함량차이유통계학의의(t =4.3311,P=0.0000),이식후여정상대조조항내피세포항체함량차이야구유통계학의의(t =5.5572,P=0.0000).결론 항내피세포항체작위비HLA항체시신이식만성배척반응적영향인소지일.
Objective To explore the role of non-HLA antibodies in chronic renal allograft rejection.Methods Immunofluoreseence assay was performed to detect anti-endothelial cell antibodies in the serum samples of 252 subjects including 72 patients receiving dialysis before renal transplantation,100 patients after renal transplantation and 80 normal blood donors.Result Anti-endothelial cell antibodies were detected in 38.89％ (28/72) patients before renal transplantation.The strong positive rate accounted for 13.89％ (10/72),and the weak positive rate accounted for 25.00％ (18/72).Anti-endothelial cell antibodies were detected in 70.00％ (70/100) patients after transplantation,in which the strong positive rate accounted for 38.00％ (38/ 100),while the weak positive rate accounted for 32.00％ (32/100).Anti-endothelial cell antibodies were detected 30.00％ (24/80) in normal blood donors,in which the strong positive rate was 10.00％ (8/80) and the weak positive rate was 20.00％ (16/80).The levels of anti-endothelial cell antibodies showed no difference between the pre-transplant group and normal control group (t =1.0105,P =0.1566).The levels of anti-endothelial cell antibodies were significantly different between pre-transplant group and post-transplant group (t =4.3311,P =0.0000).The levels of anti-endothelial cell antibodies showed significant difference between post-transplant group and normal control group (t =5.5572,P =0.0000).Conclusion Anti-endothelial cell antibodies were one of the important factors in chronic renal allograft rejection.
