CAJ | 학술논문

目的研究急性冠脉综合征(ACS)患者外周血CD4+CD28-T细胞是否表达肿瘤坏死因子凋亡诱导配体(TRAIL)以及CD4+CD28-T细胞是否通过TRAIL途径诱导人血管平滑肌细胞(VSMCs)凋亡.方法采用流式细胞术、免疫磁珠法、细胞克隆技术和Annexin-V/PI双染色法分析ACS患者外周血CD4+CD28-T细胞TRAIL表达及VSMCs凋亡.结果 ACS患者外周血CD4+CD28-T细胞表达TRAIL显著高于CD4+CD28+T细胞(t=3.54,P＜0.01).在效靶比为5∶1和20∶1时,CD4+CD28-T细胞引起VSMCs凋亡的程度显著高于CI4+CD28+T细胞(t=6.15,P＜0.05;t =5.21,P＜0.05).加入TRAIL抗体阻断后CD4+CD28-T细胞引起VSMCs凋亡的程度明显减少(t=3.22,P＜0.01).结论 ACS患者外周血CD4+CD28-T细胞数量增多而且表达TRAIL,CD4+CD28-T细胞可通过TRAIL途径诱导VSMCs凋亡.
목적 연구급성관맥종합정(ACS)환자외주혈CD4+CD28-T세포시부표체종류배사인자조망유도배체(TRAIL)이급CD4+CD28-T세포시부통과TRAIL도경유도인혈관평활기세포(VSMCs)조망.방법 채용류식세포술、면역자주법、세포극륭기술화Annexin-V/PI쌍염색법분석ACS환자외주혈CD4+CD28-T세포TRAIL표체급VSMCs조망.결과 ACS환자외주혈CD4+CD28-T세포표체TRAIL현저고우CD4+CD28+T세포(t=3.54,P＜0.01).재효파비위5∶1화20∶1시,CD4+CD28-T세포인기VSMCs조망적정도현저고우CI4+CD28+T세포(t=6.15,P＜0.05;t =5.21,P＜0.05).가입TRAIL항체조단후CD4+CD28-T세포인기VSMCs조망적정도명현감소(t=3.22,P＜0.01).결론 ACS환자외주혈CD4+CD28-T세포수량증다이차표체TRAIL,CD4+CD28-T세포가통과TRAIL도경유도VSMCs조망.
Objective To study CD4+CD28-T cells-induced apoptosis of human vascular smooth muscle cells (VSMCs) in patients with acute coronary syndrome (ACS).Methods The number of CD4+CD28-T cells and the expression of TRAIL on CD4+CD28-T cells were determined by a fluorescence-activated cell sorter (FACS).CD4+CD28-T cells were isolated using magnetic sand (MACS).These cells were cloned with limiting dilution analysis.VSMCs were incubated with CD4+CD28-T cells.VSMC apoptosis was detected using 4',6-diamidino-2-phenylindole dihydrochloride (DAPI).Results The number of CD4+CD28-T cells in the peripheral blood of the patients with ACS was higher than those in the control group and those in the stable angina pectoris (SAP) group.The expression of TRAIL on the CD4+CD28-T cells was significantly higher than that on the CD4+CD28-T cells(t =3.54,P ＜0.01).At different effector/target (E/T) ratios,the degree of VSMC apoptosis induced by the CD4+CD28-T cells was significantly higher than that induced by CD4+CD28-T cells(t =6.15,P ＜ 0.05 ;t =5.21,P ＜ 0.05).Apoptosis of VSMCs was decreased when an anti-TRAIL antibody was added(t =3.22,P ＜ 0.01).Conclusions The number of CD4+CD28-T cells expressing TRAIL was significantly increased in the peripheral blood of patients with ACS,CD4+CD28-T cells were able to induce apoptosis of VSMCs through TRAIL pathway.The elevation in the number of CD4+CD28-T cells may affect the stability of atherosclerotic plaques.