CAJ | 학술논문

目的分析山东汉族梅毒(Syphilis)患者杀伤细胞免疫球蛋白样受体(KIR)基因和人类白细胞抗原(HLA)C基因多态性,探讨其与梅毒发生之间的关联性.方法采用序列特异性引物聚合酶链反应(PCR-SSP)法,对山东汉族231例梅毒患者和247例健康个体KIR和HLA-C基因进行检测和分析.结果在检测的全部个体中,框架基因KIR2DL4、KIR3DL2、KIR3DL3和KIR3DP1的表型频率均为100％.抑制型KIR基因的表型频率在梅毒病例组和对照组中相比差别无统计学意义.梅毒病例组激活型KIR2DS3和KIR3DS1基因的表型频率均显著高于对照组(P =0.030,P=0.038),而KIR2DS5基因的表型频率显著低于对照组(P=0.015,OR=0.575).梅毒病例组纯合子(homozygote) HLA-C1C1基因型的表型频率显著低于对照组(P =0.030,OR=0.667).梅毒病例组HLA-C1C1-KIR2DL3基因型的表型频率显著低于对照组(P =0.018,OR =0.647).结论 KIR2DS3、KIR3DS1基因可能是梅毒的易感基因、KIR2DS5基因,HLA-C1C1和HLA-C1C1-KIR2DL3基因型可能是梅毒的抗性基因(型).
목적 분석산동한족매독(Syphilis)환자살상세포면역구단백양수체(KIR)기인화인류백세포항원(HLA)C기인다태성,탐토기여매독발생지간적관련성.방법 채용서렬특이성인물취합매련반응(PCR-SSP)법,대산동한족231례매독환자화247례건강개체KIR화HLA-C기인진행검측화분석.결과 재검측적전부개체중,광가기인KIR2DL4、KIR3DL2、KIR3DL3화KIR3DP1적표형빈솔균위100％.억제형KIR기인적표형빈솔재매독병례조화대조조중상비차별무통계학의의.매독병례조격활형KIR2DS3화KIR3DS1기인적표형빈솔균현저고우대조조(P =0.030,P=0.038),이KIR2DS5기인적표형빈솔현저저우대조조(P=0.015,OR=0.575).매독병례조순합자(homozygote) HLA-C1C1기인형적표형빈솔현저저우대조조(P =0.030,OR=0.667).매독병례조HLA-C1C1-KIR2DL3기인형적표형빈솔현저저우대조조(P =0.018,OR =0.647).결론 KIR2DS3、KIR3DS1기인가능시매독적역감기인、KIR2DS5기인,HLA-C1C1화HLA-C1C1-KIR2DL3기인형가능시매독적항성기인(형).
Objective To explore whether killer cell immunoglobulin-like receptor (KIR)and human leukocyte antigen (HLA) C gene polymorphisms are associated with syphilis in a Han population in Shandong area.Methods Polymerase chain reaction with sequence-specific primers (PCR-SSP) was used to genotype KIR and HLA-C genes in 231 syphilis patients and 247 healthy controls.Results Framework genes KIR2DL4,KIR3DL2,KIR3DL3 and KIR3DP1 were present in all individuals.There were no significant different distributions of inhibitory KIR genes in the two groups.The frequencies of KIR2DS3 and KIR3DS1 were higher in syphilis patients than those in healthy controls (P =0.030 and P =0.038,respectively),and the frequency of KIR2DS5 was higher in healthy controls than that in syphilis patients (P =0.015; OR =0.575).The homozygote for HLA-C1 allele (HLA-C1C1) was more common in controls compared to syphilis patients (P =0.030;OR =0.667).The frequency of individuals with HLA-C1C1 and KIR2DL3 genotype was higher in control group relative to syphilis patient group (P =0.018 ; OR =0.647).Conclusions These data suggested that KIR2DS3 and KIR3DS1 were associated with susceptibility to syphilis,while KIR2DS5,HLA-C1C1 and HLAC1C1-KIR2DL3 were associated with resistance to syphilis in the Han population in Shandong area.However,investigation using functional studies are required.