国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2013年
4期
310-313,328
,共5页
芦建慧%陈俭静%彭相文%吴坤%吴春艳%赵艳
蘆建慧%陳儉靜%彭相文%吳坤%吳春豔%趙豔
호건혜%진검정%팽상문%오곤%오춘염%조염
非酒精性脂肪肝%肿瘤坏死因子-α%体质指数
非酒精性脂肪肝%腫瘤壞死因子-α%體質指數
비주정성지방간%종류배사인자-α%체질지수
Non-alcoholic fatty liver disease%Tumor necrosis factor-α%Body mass index
目的 检测非酒精性脂肪肝病(NAFLD)患者与健康对照者体格、生化指标和血清肿瘤坏死因子-α(TNF-α)的水平,了解血清TNF-α等在NAFLD发生发展中的意义.方法 选择NAFLD患者132例,正常对照132例.测量身高、体重、腰围(WC)、血压,检测肝功、血糖(GLU)、血脂、尿酸和指标；采用酶联免疫法(ELISA)检测病例与对照者血清TNF-α水平,分析NAFLD与炎症因子TNF-α的相关性.构建Logistic回归模型分析NAFLD的影响因素.结果 NAFLD组体质指数(BMI)[(25.37±3.29) kg/m2]、腰围[(84.33±11.00)cm]、收缩压[(134.93±19.32) mmHg]、舒张压[(82.50±13.33)mmHg]和血清谷丙转氨酶(ALT)[(24.59±16.01)U/L]、谷草转氨酶(AST)[(23.88±10.56) U/L]、尿酸(UA)[(322.96±95.57) μmol/L]、甘油三酯(TG)[(1.69 ±0.96) mmol/L]等生化指标水平均明显高于对照组[分别为BMI (23.11±2.23) kg/m2、WC (78.00±6.67) cm、收缩压(122.73±17.64) mmHg、舒张压(75.35±11.52) mmHg、ALT(15.62±10.64)U/L、AST(19.79±5.68) U/L、UA(287.91±93.16)μmol/L、TG(1.23±0.74) mmol/L,P＜0.01].NAFLD患者血清TNF-α的水平[(2.78±1.46) pg/ml]明显高于对照组[(2.17±1.21) pg/ml],差异有统计学意义(P均＜0.05)；NAFLD与血清TNF-α水平均呈正相关[r值为0.185,P＜0.01].两组TNF-α、BMI、WC、SBP、DBP、ALT、AST、UA、TG水平的差异均有统计学意义(均为P＜0.05),而GLU、总胆固醇(CHOL)差异无统计学意义(P＞0.05).多因素Logistic回归分析显示血清TNF-α、BMI、TG是NAFLD的危险因素,OR(95％ CI)分别为1.826(1.708～2.664)、1.689(1.542～1.891)、1.437(1.127～1.737),P值均＜0.05).结论 非酒精性脂肪肝病患者血清TNF-α水平明显升高,提示TNF-α可能在非酒精性脂肪肝的发生发展中起重要作用.
目的 檢測非酒精性脂肪肝病(NAFLD)患者與健康對照者體格、生化指標和血清腫瘤壞死因子-α(TNF-α)的水平,瞭解血清TNF-α等在NAFLD髮生髮展中的意義.方法 選擇NAFLD患者132例,正常對照132例.測量身高、體重、腰圍(WC)、血壓,檢測肝功、血糖(GLU)、血脂、尿痠和指標；採用酶聯免疫法(ELISA)檢測病例與對照者血清TNF-α水平,分析NAFLD與炎癥因子TNF-α的相關性.構建Logistic迴歸模型分析NAFLD的影響因素.結果 NAFLD組體質指數(BMI)[(25.37±3.29) kg/m2]、腰圍[(84.33±11.00)cm]、收縮壓[(134.93±19.32) mmHg]、舒張壓[(82.50±13.33)mmHg]和血清穀丙轉氨酶(ALT)[(24.59±16.01)U/L]、穀草轉氨酶(AST)[(23.88±10.56) U/L]、尿痠(UA)[(322.96±95.57) μmol/L]、甘油三酯(TG)[(1.69 ±0.96) mmol/L]等生化指標水平均明顯高于對照組[分彆為BMI (23.11±2.23) kg/m2、WC (78.00±6.67) cm、收縮壓(122.73±17.64) mmHg、舒張壓(75.35±11.52) mmHg、ALT(15.62±10.64)U/L、AST(19.79±5.68) U/L、UA(287.91±93.16)μmol/L、TG(1.23±0.74) mmol/L,P＜0.01].NAFLD患者血清TNF-α的水平[(2.78±1.46) pg/ml]明顯高于對照組[(2.17±1.21) pg/ml],差異有統計學意義(P均＜0.05)；NAFLD與血清TNF-α水平均呈正相關[r值為0.185,P＜0.01].兩組TNF-α、BMI、WC、SBP、DBP、ALT、AST、UA、TG水平的差異均有統計學意義(均為P＜0.05),而GLU、總膽固醇(CHOL)差異無統計學意義(P＞0.05).多因素Logistic迴歸分析顯示血清TNF-α、BMI、TG是NAFLD的危險因素,OR(95％ CI)分彆為1.826(1.708～2.664)、1.689(1.542～1.891)、1.437(1.127～1.737),P值均＜0.05).結論 非酒精性脂肪肝病患者血清TNF-α水平明顯升高,提示TNF-α可能在非酒精性脂肪肝的髮生髮展中起重要作用.
목적 검측비주정성지방간병(NAFLD)환자여건강대조자체격、생화지표화혈청종류배사인자-α(TNF-α)적수평,료해혈청TNF-α등재NAFLD발생발전중적의의.방법 선택NAFLD환자132례,정상대조132례.측량신고、체중、요위(WC)、혈압,검측간공、혈당(GLU)、혈지、뇨산화지표；채용매련면역법(ELISA)검측병례여대조자혈청TNF-α수평,분석NAFLD여염증인자TNF-α적상관성.구건Logistic회귀모형분석NAFLD적영향인소.결과 NAFLD조체질지수(BMI)[(25.37±3.29) kg/m2]、요위[(84.33±11.00)cm]、수축압[(134.93±19.32) mmHg]、서장압[(82.50±13.33)mmHg]화혈청곡병전안매(ALT)[(24.59±16.01)U/L]、곡초전안매(AST)[(23.88±10.56) U/L]、뇨산(UA)[(322.96±95.57) μmol/L]、감유삼지(TG)[(1.69 ±0.96) mmol/L]등생화지표수평균명현고우대조조[분별위BMI (23.11±2.23) kg/m2、WC (78.00±6.67) cm、수축압(122.73±17.64) mmHg、서장압(75.35±11.52) mmHg、ALT(15.62±10.64)U/L、AST(19.79±5.68) U/L、UA(287.91±93.16)μmol/L、TG(1.23±0.74) mmol/L,P＜0.01].NAFLD환자혈청TNF-α적수평[(2.78±1.46) pg/ml]명현고우대조조[(2.17±1.21) pg/ml],차이유통계학의의(P균＜0.05)；NAFLD여혈청TNF-α수평균정정상관[r치위0.185,P＜0.01].량조TNF-α、BMI、WC、SBP、DBP、ALT、AST、UA、TG수평적차이균유통계학의의(균위P＜0.05),이GLU、총담고순(CHOL)차이무통계학의의(P＞0.05).다인소Logistic회귀분석현시혈청TNF-α、BMI、TG시NAFLD적위험인소,OR(95％ CI)분별위1.826(1.708～2.664)、1.689(1.542～1.891)、1.437(1.127～1.737),P치균＜0.05).결론 비주정성지방간병환자혈청TNF-α수평명현승고,제시TNF-α가능재비주정성지방간적발생발전중기중요작용.
Objective To detect and evaluate the significance of physical indicator,biochemical indicator and tumor necrosis factor alpha (TNF-α) in the serum of patients with non-alcoholic fatty liver disease (NAFLD).Methods 132 NAFLD patients and 132 healthy individuals were recruited.Height,body weight,waist circumference (WC),systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured.The levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),fasting serum glucose (GLU),triglyceride (TG),total cholesterol (TC) and uric acid (UA) were detected.Serum TNF-α levels were determined by enzyme-linked immunosorbent assay (ELISA) in patients with NAFLD and controls.The correlation between NAFLD and serum TNF-α was analyzed by Spearman correlation analysis.The risk factors of NAFLD were analyzed by logistic regression models.Results BMI [(25.37 ± 3.29) kg/m2],WC [(84.33 ± 11.00) cm],SBP [(134.93 ± 19.32) mmHg],DBP [(82.50 ± 13.33) mmHg],the levels of ALT [(24.59 ± 16.01) U/L],AST [(23.88 ± 10.56) U/L],UA [(322.96 ± 95.57) μmol/L],triglyceride [(1.69 ± 0.96) mmol/L] in the NAFLD group were evidently higher than those in control group [23.11 ±2.23 kg/m2,(78.00 ± 6.67) cm,(122.73 ± 17.64) mmHg,(75.35 ± 11.52) mmHg,(15.62 ± 10.64) U/L,(19.79 ± 5.68) U/L,(287.91 ± 93.16) μmol/L,(1.23 ± 0.74) mmol/L,respectively,all P ＜ 0.01].Serum TNF-α levels in the NAFLD group [(2.78 ± 1.46) pg/ml] were evidently higher than those in control group [(2.17 ± 1.21) pg/ml,P ＜ 0.05] and positively correlated with NAFLD (r value was 0.185,P ＜ 0.01).The levels of TNF-α,BMI,WC,SBP,DBP,ALT,AST,UA and TG were significantly different between the two groups (P ＜ 0.05),but the levels of GLU and CHOL were not significantly different.Multivariate logistic regress results showed that the levels of TNF-α,BMI and TG were the risk factors of NAFLD with [OR (95％ CI) of 1.826 (1.708-2.664),1.689 (1.542-1.891) and 1.437 (1.127-1.737),respectively,all P ＜ 0.05].Conclusion The levels of TNF-α were significantly increased in NAFLD patients.These results suggest that TNF-α might play an important role in the pathogenesis of non-alcoholic fatty liver disease.
