CAJ | 학술논문

目的探讨一氧化氮(NO)供体药物硝普钠腹腔注射特发性血小板减少性紫癜(ITP)模型小鼠后,对不同时间点小鼠脾脏内还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)的影响及意义.方法 ITP模型小鼠分成NO供体药物硝普钠(SNP)组和磷酸盐缓冲液(PBS)对照组,SNP组用相同浓度(1 mg/ml)的NO供体药硝普钠稀释液进行腹腔注射,PBS对照组使用相同体积的磷酸盐缓冲液进行腹腔注射,用药第5 min、15 min、25 min、35 min和45 min后,检测外周血中NO及一氧化氮合酶(NOS)的表达,酶联免疫法(ELISA)法测定脾脏中GSH和GSSG含量.结果 SNP组小鼠血清中NO及NOS在第5 min,15 min,25 min,35 min和45 min的浓度均高于PBS对照组(NO:t5 =6.24,t15=8.65,t25=9.87,t35=11.20,t45=13.07; NOS:t5 =5.47,t15=6.55,t25=8.04,t35=9.28,t45=11.54,P均＜0.05),相邻测量时间点NO及NOS含量均具有统计学意义(NO:t5-15=3.04,t15-25 =4.11,t25-35=3.82,t35-45=3.54；NOS:t5-15=3.98,t15-25=5.04,t25-35 =5.12,t35-45 =4.26,P均＜0.05),GSH浓度随着时间的延长而降低,相邻时间点测量值有统计学意义(t5-15=5.62,t15-25=4.90,t25-35=5.21,t35-45=5.07,P均＜0.05),GSSG的含量随着时间的延长而升高,相邻时间点测量值有着显著性差异(t5-15=4.07,t15-25=6.57,t25-35=4.98,t35-45=5.41,P均＜0.05),GSH浓度分别与NO、NOS呈负相关(r=-0.625,r=-0.534,均P＜0.01),GSSG浓度分别与NO、NOS呈正相关(r=0.714,r=0.687,均P＜0.01).结论在ITP小鼠模型体内,NO供体药物硝普钠以时间依赖的形式减少GSH的含量,硝普钠可通过影响抗氧化系统GSH/GSSG来发挥相应的生理功能.
목적 탐토일양화담(NO)공체약물초보납복강주사특발성혈소판감소성자전(ITP)모형소서후,대불동시간점소서비장내환원형곡광감태(GSH)화양화형곡광감태(GSSG)적영향급의의.방법 ITP모형소서분성NO공체약물초보납(SNP)조화린산염완충액(PBS)대조조,SNP조용상동농도(1 mg/ml)적NO공체약초보납희석액진행복강주사,PBS대조조사용상동체적적린산염완충액진행복강주사,용약제5 min、15 min、25 min、35 min화45 min후,검측외주혈중NO급일양화담합매(NOS)적표체,매련면역법(ELISA)법측정비장중GSH화GSSG함량.결과 SNP조소서혈청중NO급NOS재제5 min,15 min,25 min,35 min화45 min적농도균고우PBS대조조(NO:t5 =6.24,t15=8.65,t25=9.87,t35=11.20,t45=13.07; NOS:t5 =5.47,t15=6.55,t25=8.04,t35=9.28,t45=11.54,P균＜0.05),상린측량시간점NO급NOS함량균구유통계학의의(NO:t5-15=3.04,t15-25 =4.11,t25-35=3.82,t35-45=3.54；NOS:t5-15=3.98,t15-25=5.04,t25-35 =5.12,t35-45 =4.26,P균＜0.05),GSH농도수착시간적연장이강저,상린시간점측량치유통계학의의(t5-15=5.62,t15-25=4.90,t25-35=5.21,t35-45=5.07,P균＜0.05),GSSG적함량수착시간적연장이승고,상린시간점측량치유착현저성차이(t5-15=4.07,t15-25=6.57,t25-35=4.98,t35-45=5.41,P균＜0.05),GSH농도분별여NO、NOS정부상관(r=-0.625,r=-0.534,균P＜0.01),GSSG농도분별여NO、NOS정정상관(r=0.714,r=0.687,균P＜0.01).결론 재ITP소서모형체내,NO공체약물초보납이시간의뢰적형식감소GSH적함량,초보납가통과영향항양화계통GSH/GSSG래발휘상응적생리공능.
Objective To investigate the impact of sodium nitroprusside (SNP) on the concentration of antioxidants glutathione(GSH) and oxidized glutathione (GSSG) in the spleens of idiopathic thrombocytopenia purpura (ITP).Method ITP model mice were divided into the nitric oxide (NO) donor drug SNP groups and phosphate buffered saline (PBS) control group.Intraperitoneal injection with the concentration (1 mg/ml) of SNP for SNP group while intraperitoneal injection using the same volume of PBS for PBS control group.The expression of NO,nitric oxide synthase (NOS) in peripheral blood and GSH / GSSG content in spleen was detected after medication for 5 min,15 min,25 min,35 min and 45min.Results Serum concentration of NO and NOS in SNP group were higher than the PBS control group in the first 5 min,15 min,25 min,35 min and 45min (NO:t5 =6.24,t15 =8.65,t25 =9.87,t35 =11.20,t45 =13.07;NOS:t5 =5.47,t15 =6.55,t25 =8.04,t35 =9.28,t45 =11.54,all P ＜ 0.05).NO and NOS content had statistical difference in each measurement time point (NO:t5-15 =3.04,t15-25 =4.11,t25-35 =3.82,t35-45 =3.54;NOS:t5-15 =3.98,t15-25 =5.04,t25-35 =5.12,t35-45 =4.26,all P ＜ 0.05).GSH concentration reduced with time while GSSG content increased with time (GSH:t5-15 =5.62,t15-25 =4.90,t25-35 =5.21,t35-45 =5.07;GSSG:t5-15 =4.07,t15-25 =6.57,t25-35 =4.98,t35-45 =5.41,all P ＜ 0.05).GSH concentrations were negatively correlated with NO,NOS (r =-0.625,r =-0.534,both P ＜ 0.01).GSSG concentrations were positively correlated with NO,NOS (r =0.714,r =0.687,both P ＜ 0.01).Conclusion NO donor drug SNP reducd the level of GSH in a time-dependent pattern in ITP mice,SNP may play a physiological function by affecting the system of antioxidant GSH / GSSG.