国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2013年
5期
397-401
,共5页
马树立%李芳%徐彪%梁含思%孙青%秦明德%张学光
馬樹立%李芳%徐彪%樑含思%孫青%秦明德%張學光
마수립%리방%서표%량함사%손청%진명덕%장학광
羊膜间充质干细胞%绒毛膜间充质干细胞%程序性死亡配体-1%神经特异性蛋白
羊膜間充質榦細胞%絨毛膜間充質榦細胞%程序性死亡配體-1%神經特異性蛋白
양막간충질간세포%융모막간충질간세포%정서성사망배체-1%신경특이성단백
Amniotic mesenchymal stem cells%Chorionic Mesenchymal stem cells%Programmd death ligand-1%Neuron-specific protein
目的 分析比较胎盘不同组织来源间充质干细胞(MSCs)的生物学特性及其分化潜能.方法 建立羊膜间充质(AMSCs)、绒毛膜间充质(CMSCs)体外扩增体系,流式细胞仪分析两群MSCs细胞表型,免疫组织化学染色观察绒毛膜中CMSCs分布及负性共刺激分子程序性死亡配体-1(PD-L1)表达,将两群MSCs分别向神经元诱导,RT-PCR检测诱导前后不同神经特异性蛋白的表达情况.结果 AMSCs与CMSCs均高表达CD90、CD73和CD105,不表达CD14、CD34、CD45和HLA-DR.与此同时,CMSCs表达协同刺激分子PD-L1,而AMSCs不表达；AMSCs本身表达Musashi-1、Nestin、β-tubulinⅢ和胶质原纤维酸性蛋白(GFAP),诱导后较易出现神经丝蛋白(NF)的表达；绒毛膜中可见CD90、CD166及PD-L1阳性表达的CMSCs紧贴滋养细胞分布.结论 AMSCs具有良好的神经生物学特性,在特定条件下较CMSCs更易于向神经元或神经胶质细胞分化,CMSCs则是作为研究MSCs免疫调节机制理想的细胞来源.
目的 分析比較胎盤不同組織來源間充質榦細胞(MSCs)的生物學特性及其分化潛能.方法 建立羊膜間充質(AMSCs)、絨毛膜間充質(CMSCs)體外擴增體繫,流式細胞儀分析兩群MSCs細胞錶型,免疫組織化學染色觀察絨毛膜中CMSCs分佈及負性共刺激分子程序性死亡配體-1(PD-L1)錶達,將兩群MSCs分彆嚮神經元誘導,RT-PCR檢測誘導前後不同神經特異性蛋白的錶達情況.結果 AMSCs與CMSCs均高錶達CD90、CD73和CD105,不錶達CD14、CD34、CD45和HLA-DR.與此同時,CMSCs錶達協同刺激分子PD-L1,而AMSCs不錶達；AMSCs本身錶達Musashi-1、Nestin、β-tubulinⅢ和膠質原纖維痠性蛋白(GFAP),誘導後較易齣現神經絲蛋白(NF)的錶達；絨毛膜中可見CD90、CD166及PD-L1暘性錶達的CMSCs緊貼滋養細胞分佈.結論 AMSCs具有良好的神經生物學特性,在特定條件下較CMSCs更易于嚮神經元或神經膠質細胞分化,CMSCs則是作為研究MSCs免疫調節機製理想的細胞來源.
목적 분석비교태반불동조직래원간충질간세포(MSCs)적생물학특성급기분화잠능.방법 건립양막간충질(AMSCs)、융모막간충질(CMSCs)체외확증체계,류식세포의분석량군MSCs세포표형,면역조직화학염색관찰융모막중CMSCs분포급부성공자격분자정서성사망배체-1(PD-L1)표체,장량군MSCs분별향신경원유도,RT-PCR검측유도전후불동신경특이성단백적표체정황.결과 AMSCs여CMSCs균고표체CD90、CD73화CD105,불표체CD14、CD34、CD45화HLA-DR.여차동시,CMSCs표체협동자격분자PD-L1,이AMSCs불표체；AMSCs본신표체Musashi-1、Nestin、β-tubulinⅢ화효질원섬유산성단백(GFAP),유도후교역출현신경사단백(NF)적표체；융모막중가견CD90、CD166급PD-L1양성표체적CMSCs긴첩자양세포분포.결론 AMSCs구유량호적신경생물학특성,재특정조건하교CMSCs경역우향신경원혹신경효질세포분화,CMSCs칙시작위연구MSCs면역조절궤제이상적세포래원.
Objective To analyze the biological characteristics and differentiation potential of mesenchymal stem cells derived from different placenta tissues.Methods We established a system to amplify amniotic mesenchymal stem cells (AMSCs) and chorionic mesenchymal stem cells(CMSCs) in vitro and analyzed the cell phenotype of the two groups of mesenchymal stem cells (MSCs) by flow cytometry.We observed the distribution of CMSCs in chorionic and the expression of programmd death ligand-1 (PD-L1) by immunohistochemistry.We also detected the expression of neuron-specific protein in the two groups of MSCs before and after induction by RT-PCR.Results Both MACs and CMSCs expressed CD90,CD73,CD105 in high level,but not CD14,CD34,CD45,HLA-DR.Costimulatory molecule PD-L1 was found in CMSCs,but not in AMSCs.The CMSCs with positive expression of CD90 and CD166 were located in close proximity to the trophoblast cells.Before induction,Musashi-1,Nestin,β-tubulin Ⅲ (Tuj1) and GFAP mRNA expression were found in AMSCs,while only Nestin mRNA was detected in CMSCs.2 days after induction,musashi-1,nestin,β-tubulin Ⅲ,glil fibrillary acidic protein (GFAP),neurafilament protein (NF) mRNA expression was also detected in AMSCs,while Nestin could be detected in CMSCs.5 days after induction,the neural-specific protein expression pattern in AMSCs was almost the same as that at 2 days after induction except declined Nestin expression,while NF and GFAP expression could be detected in CMSCs.Conclusion AMSCs have good neurobiology properties and are easier to differentiate to neurons or glial cells under certain conditions.CMSCs could be the better source of cells to study the immunomodulatory mechanism of MSCs.
