国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2014年
1期
74-77
,共4页
李妍%李迪%杨丹%王甲业%庄敏%凌虹
李妍%李迪%楊丹%王甲業%莊敏%凌虹
리연%리적%양단%왕갑업%장민%릉홍
人类免疫缺陷病毒-1%包膜糖蛋白%V4区%CD4+ T细胞数
人類免疫缺陷病毒-1%包膜糖蛋白%V4區%CD4+ T細胞數
인류면역결함병독-1%포막당단백%V4구%CD4+ T세포수
Human immunodeficiency virus type 1%Envelope%V4 region%CD4+ T cell
目的 探讨人类免疫缺陷病毒感染者包膜蛋白V4区变异与CD4+ T细胞数之间的关系.方法 本研究从Los Alamos HIV序列数据库中选取369条HIV-1 B亚型且已知CD4+ T细胞数的V4区氨基酸序列.分析CD4+ T细胞数与V4区氨基酸序列长度、潜在糖基化位点(PNGS)及净电荷数的相关性.V4区氨基酸序列按CD4+ T细胞数高低分成两组:CD4+ T细胞数＜ 200个/μL组(n=216)和-CD4+ T细胞数≥200个/μL组(n=153),比较两组V4区氨基酸的差异.结果 CD4+ T细胞数与V4区氨基酸序列长度及PNGS数正相关(r=0.248,0.136,P均＜0.01),而与V4区净电荷数负相关(r=-0.154,P＜0.01).CD4+ T细胞数＜200个/μL与CD4+ T细胞数＞ 200个/μL组相比,V4区氨基酸序列长度较短(P＜0.01)、PNGS数较少(P＜0.05).但CD4+ T细胞数高低两组V4区净电荷数之间无统计学意义(P=0.17).结论 V4区变异和HIV-1感染的病程具有相关性,随着CD4+ T细胞数的降低,V4区序列变短、PNGS数减少.
目的 探討人類免疫缺陷病毒感染者包膜蛋白V4區變異與CD4+ T細胞數之間的關繫.方法 本研究從Los Alamos HIV序列數據庫中選取369條HIV-1 B亞型且已知CD4+ T細胞數的V4區氨基痠序列.分析CD4+ T細胞數與V4區氨基痠序列長度、潛在糖基化位點(PNGS)及淨電荷數的相關性.V4區氨基痠序列按CD4+ T細胞數高低分成兩組:CD4+ T細胞數＜ 200箇/μL組(n=216)和-CD4+ T細胞數≥200箇/μL組(n=153),比較兩組V4區氨基痠的差異.結果 CD4+ T細胞數與V4區氨基痠序列長度及PNGS數正相關(r=0.248,0.136,P均＜0.01),而與V4區淨電荷數負相關(r=-0.154,P＜0.01).CD4+ T細胞數＜200箇/μL與CD4+ T細胞數＞ 200箇/μL組相比,V4區氨基痠序列長度較短(P＜0.01)、PNGS數較少(P＜0.05).但CD4+ T細胞數高低兩組V4區淨電荷數之間無統計學意義(P=0.17).結論 V4區變異和HIV-1感染的病程具有相關性,隨著CD4+ T細胞數的降低,V4區序列變短、PNGS數減少.
목적 탐토인류면역결함병독감염자포막단백V4구변이여CD4+ T세포수지간적관계.방법 본연구종Los Alamos HIV서렬수거고중선취369조HIV-1 B아형차이지CD4+ T세포수적V4구안기산서렬.분석CD4+ T세포수여V4구안기산서렬장도、잠재당기화위점(PNGS)급정전하수적상관성.V4구안기산서렬안CD4+ T세포수고저분성량조:CD4+ T세포수＜ 200개/μL조(n=216)화-CD4+ T세포수≥200개/μL조(n=153),비교량조V4구안기산적차이.결과 CD4+ T세포수여V4구안기산서렬장도급PNGS수정상관(r=0.248,0.136,P균＜0.01),이여V4구정전하수부상관(r=-0.154,P＜0.01).CD4+ T세포수＜200개/μL여CD4+ T세포수＞ 200개/μL조상비,V4구안기산서렬장도교단(P＜0.01)、PNGS수교소(P＜0.05).단CD4+ T세포수고저량조V4구정전하수지간무통계학의의(P=0.17).결론 V4구변이화HIV-1감염적병정구유상관성,수착CD4+ T세포수적강저,V4구서렬변단、PNGS수감소.
Objective To characterize the correlation between the variation of V4 sequence and CD4+ T cell counts.Methods 369 V4 sequences with CD4+ T cell counts known were downloaded from the Los Alamos HIV Sequence Database.Correlations between CD4+ T cell counts and length,the number of PNGS,net charge were assessed for univariate comparisons.Furthermore,All the sequences were classified into high CD4+ T cell counts (≥200/μL) group and low CD4+ T cell counts group (＜ 200/μL).No-parametric test was used to discover the different between the two groups.Results There were significant positive correlations between the CD4+ T cell counts and length,PNGS(r =0.248,0.136,P ＜ 0.01).However,the results showed a negative association between the CD4+ T cell counts and net charge(r =-0.154,P ＜0.01).Moreover,there were less number of amino acids (P ＜ 0.01) and PNGS (P ＜ 0.05) of V4 sequences with CD4+ T cell counts ＜ 200 cells/μL.Conclusion The length and PNGS of V4 were decreased with the decline of CD4+ T cell counts,which indicated that there was a correlation between the variation of V4 and CD4+ T cell counts.
