CAJ | 학술논문

目的探讨兔抗鼠Fx1A抗体诱导膜性肾病大鼠模型的方法及病理特点.方法 SD雄性大白鼠64只随机分为正常对照组(18只)、正常兔血清组(18只)、模型组(28只),正常对照组大鼠腹腔一次性注入生理盐水8 mL/kg,正常兔血清组大鼠腹腔一次性注入正常兔血清8 mL/kg,模型组大鼠腹腔一次注入抗Fx1A 8 mL/kg.模型组1周后测定24 h尿蛋白(24 h UPQ)含量,小于5 mg不予采用(10只).第2周、第4周各组随机选取9只大鼠,检测24 h UPQ及生化指标变化,光镜、荧光显微镜、电镜观察肾脏病理改变.结果第2、4周末模型组大鼠出现大量蛋白尿,血清白蛋白明显下降,胆固醇、三酰甘油明显升高(P＜0.05);光镜、免疫荧光、电镜与膜性肾病病理表现相符.结论本实验腹腔注射兔抗鼠Fx1A抗体形成的大鼠模型临床表现及病理学特点均酷似人类膜性肾病.
목적 탐토토항서Fx1A항체유도막성신병대서모형적방법급병리특점.방법 SD웅성대백서64지수궤분위정상대조조(18지)、정상토혈청조(18지)、모형조(28지),정상대조조대서복강일차성주입생리염수8 mL/kg,정상토혈청조대서복강일차성주입정상토혈청8 mL/kg,모형조대서복강일차주입항Fx1A 8 mL/kg.모형조1주후측정24 h뇨단백(24 h UPQ)함량,소우5 mg불여채용(10지).제2주、제4주각조수궤선취9지대서,검측24 h UPQ급생화지표변화,광경、형광현미경、전경관찰신장병리개변.결과 제2、4주말모형조대서출현대량단백뇨,혈청백단백명현하강,담고순、삼선감유명현승고(P＜0.05);광경、면역형광、전경여막성신병병리표현상부.결론 본실험복강주사토항서Fx1A항체형성적대서모형림상표현급병이학특점균혹사인류막성신병.
Objective To investigate the pathological features in the rat membranous nephropathy model induced by rabbit anti-rat FxlA.Mehtods 64 Sprague-Dawley (S-D) male rats were used for trial.18 of them were randomly assigned as the normal group and another 18 as the normal rabbit serum group.28 rats were assigned as the model group.The rats in normal group were given a single peritoneal injection of 8 mL/kg body wt of physiological saline.The rats in normal rabbit serum group were given a single peritoneal injection of 8 mL/kg body wt of normal rabbit serum.The rats of model group were given a single peritoneal injection of 8mL/kg body wt of rabbit anti-rat FxlA antibody.One week after the injection,24 hour urine protein excretion quantity (24 h UPQ) were detected for the rats in model group.The results were abandoned if their 24 h UPQ were less than 5 mg.At the end of the 2nd and 4th week,9 rats were randomly chosen from each groups and their 24 h UPQ,biochemical index and pathology changes of renal were detected.Results 24 h UPQ,CHOL and TG were significantly higher in model group than those in other groups at the the end of the 2nd and 4th week (P ＜ 0.05).ALB were significantly lower in model group than those in other groups at the end of the 2nd and 4th week(P ＜0.05).The renal histopathology of the rats in model group under the light microscope,immunofluorescence microscope,and electron microscope showed the typical features of membranous nephropathy.Conclusions The pathological features of the rat model induced by once intraperitoneal injection of rabbit antirat Fx1A serum were similar to human membranous nephropathy.