国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2014年
3期
239-241
,共3页
杨秀华%安婷婷%李子卓%张久维%张羽
楊秀華%安婷婷%李子卓%張久維%張羽
양수화%안정정%리자탁%장구유%장우
肝细胞癌%细胞凋亡%CD8+T淋巴细胞%调节性T细胞
肝細胞癌%細胞凋亡%CD8+T淋巴細胞%調節性T細胞
간세포암%세포조망%CD8+T림파세포%조절성T세포
Hepatocellular carcinoma%Apoptosis%CD8 +T lymphocyte%Regulatory T cell
目的 研究肝细胞癌患者调节性T细胞(Treg)对CD8+T淋巴细胞凋亡的影响.方法 采集20例肝细胞癌患者和20例正常人的外周血,免疫磁珠分离法分别分离肝细胞癌患者Treg和正常人Treg及CD8+T淋巴细胞,用流式分析法分别检测与肝细胞癌患者Treg及正常人Treg培养72 h后CD8+T淋巴细胞的凋亡情况.结果 与正常人Treg共培养72 h后,CD8+T淋巴细胞凋亡率为(78.2±5.79)%,与肝细胞癌患者Treg共培养72 h后CD8+T淋巴细胞凋亡率为(93.2±4.87)%,两者比较有统计学意义(t =7.54,P<0.01).结论 肝细胞癌患者免疫抑制性Treg可使CD8+T淋巴细胞的凋亡率增加.
目的 研究肝細胞癌患者調節性T細胞(Treg)對CD8+T淋巴細胞凋亡的影響.方法 採集20例肝細胞癌患者和20例正常人的外週血,免疫磁珠分離法分彆分離肝細胞癌患者Treg和正常人Treg及CD8+T淋巴細胞,用流式分析法分彆檢測與肝細胞癌患者Treg及正常人Treg培養72 h後CD8+T淋巴細胞的凋亡情況.結果 與正常人Treg共培養72 h後,CD8+T淋巴細胞凋亡率為(78.2±5.79)%,與肝細胞癌患者Treg共培養72 h後CD8+T淋巴細胞凋亡率為(93.2±4.87)%,兩者比較有統計學意義(t =7.54,P<0.01).結論 肝細胞癌患者免疫抑製性Treg可使CD8+T淋巴細胞的凋亡率增加.
목적 연구간세포암환자조절성T세포(Treg)대CD8+T림파세포조망적영향.방법 채집20례간세포암환자화20례정상인적외주혈,면역자주분리법분별분리간세포암환자Treg화정상인Treg급CD8+T림파세포,용류식분석법분별검측여간세포암환자Treg급정상인Treg배양72 h후CD8+T림파세포적조망정황.결과 여정상인Treg공배양72 h후,CD8+T림파세포조망솔위(78.2±5.79)%,여간세포암환자Treg공배양72 h후CD8+T림파세포조망솔위(93.2±4.87)%,량자비교유통계학의의(t =7.54,P<0.01).결론 간세포암환자면역억제성Treg가사CD8+T림파세포적조망솔증가.
Objeetive To study the influence regulatory T cells (Treg) of patients with hepatocellular carcinoma(HCC) on the apoptosis of CD8 +T lymphocytes.Methods Treg cells from healthy subjects and HCC patients by magnetic bead cell sorting (MACS),mixed with CD8 + T lymphocytes obtained from healthy subjects cultured 72 hours.Apoptosis of CD8 + T lymphocytes was detected by flow cytometry.Results Apoptosis of CD8 + T lymphocytes cultured with Treg cells from HCC patients (93.2 ± 4.87) % were significantly increased compared with those cultured with Treg cells from healthy subjects (78.2 ± 5.79) % (t =7.54,P <0.01).Conclusion The Apoptosis of CD8 + T lymphocytes was significantly increased by Treg cells.