CAJ | 학술논문

目的分析正常小鼠和H22肝癌小鼠血清之间差异表达的蛋白并对部分差异蛋白进行鉴定分析,筛选可能与肝癌发生密切相关的蛋白质.方法利用双向凝胶电泳技术(2-DE)与基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS),分析正常组和肿瘤组筛选并鉴定部分差异表达的蛋白,再进行生物学分析.结果通过2-DE图谱研究发现,与正常组相比,肿瘤组存在明显差异的蛋白质点.选择其中可能为关键功能蛋白的蛋白点进行质谱分析,获得肽指纹图谱(PMF),运用Mascot软件查询NCBI数据库分析并鉴定得出其中2个蛋白:假尿苷合成酶和线粒体核糖体大亚基蛋白.结论肝癌的发生机制与假尿苷合成酶和线粒体核糖体大亚基蛋白2个差异表达蛋白密切相关,其参与肝癌的发生发展.
목적 분석정상소서화H22간암소서혈청지간차이표체적단백병대부분차이단백진행감정분석,사선가능여간암발생밀절상관적단백질.방법 이용쌍향응효전영기술(2-DE)여기질보조격광해흡전리비행시간질보(MALDI-TOF-MS),분석정상조화종류조사선병감정부분차이표체적단백,재진행생물학분석.결과 통과2-DE도보연구발현,여정상조상비,종류조존재명현차이적단백질점.선택기중가능위관건공능단백적단백점진행질보분석,획득태지문도보(PMF),운용Mascot연건사순NCBI수거고분석병감정득출기중2개단백:가뇨감합성매화선립체핵당체대아기단백.결론 간암적발생궤제여가뇨감합성매화선립체핵당체대아기단백2개차이표체단백밀절상관,기삼여간암적발생발전.
Objective To analyze the expression difference of serum proteins between healthy mice and ones bearing H22 Hepatocellular carcinoma(HCC).Screen out intimate HCC-associated proteins.Methods Total protein expression of tumor and normal tissues were detected with the two-dimensional gel electrophoresis(2-DE) and mass spectrometry(MALDI-TOF-MS) assay.Differentially expressed proteins were then identified and put into further biological analysis.Results Compared with the normal control,HCC group presented remarkable variant protein spots.Two proteins that pseudouridine synthase and mitochondrial ribosomal protein L24 were obtained by PMF,Mascot software and the NCBI database.Conclusions The pseudouridine synthase and mitochondrial ribosomal protein L24 may contribute to the risk of HCC.