国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2014年
4期
341-344
,共4页
束余声%王伟奇%孙超%史宏灿%石维平
束餘聲%王偉奇%孫超%史宏燦%石維平
속여성%왕위기%손초%사굉찬%석유평
季铵盐壳聚糖%增殖%侵袭力%基质金属蛋白酶-9
季銨鹽殼聚糖%增殖%侵襲力%基質金屬蛋白酶-9
계안염각취당%증식%침습력%기질금속단백매-9
Hydroxypropyltrimethylammonium chloride chitosan%Proliferation%Invasion%Matrix metalloproteinase-9
目的 研究季铵盐壳聚糖对A549细胞增殖能力、侵袭力的影响及机制.方法 使用含有不同浓度季铵盐壳聚糖的RPMI1640培养基培养肺癌A549细胞,采用台盼蓝拒染法检测A549细胞的存活率,MTT法检测季铵盐壳聚糖对A549细胞生长的抑制率,Western Blot检测基质金属蛋白酶基质金属蛋白酶9(MMP-9)的表达情况.结果 台盼蓝拒染实验显微镜下细胞计数显示,A549细胞的存活率大于95％,满足实验要求.MTT实验中随着季铵盐壳聚糖浓度的增高(分别为50,100,200 μg/mL)对A549细胞生长的抑制率的逐渐增强[分别为(27.57±0.037)％,(66.69±0.40)％,(81.55±0.72)％],MMP-9蛋白表达水平也随之降低,呈剂量依赖性,实验组与对照组比较有差异有统计学意义(P＜0.05).结论 季铵盐壳聚糖能够抑制人非小细胞肺癌A549细胞的增殖,减少MMP-9的表达,进而降低肿瘤细胞的侵袭、转移能力,其作用效应呈剂量依赖性.
目的 研究季銨鹽殼聚糖對A549細胞增殖能力、侵襲力的影響及機製.方法 使用含有不同濃度季銨鹽殼聚糖的RPMI1640培養基培養肺癌A549細胞,採用檯盼藍拒染法檢測A549細胞的存活率,MTT法檢測季銨鹽殼聚糖對A549細胞生長的抑製率,Western Blot檢測基質金屬蛋白酶基質金屬蛋白酶9(MMP-9)的錶達情況.結果 檯盼藍拒染實驗顯微鏡下細胞計數顯示,A549細胞的存活率大于95％,滿足實驗要求.MTT實驗中隨著季銨鹽殼聚糖濃度的增高(分彆為50,100,200 μg/mL)對A549細胞生長的抑製率的逐漸增彊[分彆為(27.57±0.037)％,(66.69±0.40)％,(81.55±0.72)％],MMP-9蛋白錶達水平也隨之降低,呈劑量依賴性,實驗組與對照組比較有差異有統計學意義(P＜0.05).結論 季銨鹽殼聚糖能夠抑製人非小細胞肺癌A549細胞的增殖,減少MMP-9的錶達,進而降低腫瘤細胞的侵襲、轉移能力,其作用效應呈劑量依賴性.
목적 연구계안염각취당대A549세포증식능력、침습력적영향급궤제.방법 사용함유불동농도계안염각취당적RPMI1640배양기배양폐암A549세포,채용태반람거염법검측A549세포적존활솔,MTT법검측계안염각취당대A549세포생장적억제솔,Western Blot검측기질금속단백매기질금속단백매9(MMP-9)적표체정황.결과 태반람거염실험현미경하세포계수현시,A549세포적존활솔대우95％,만족실험요구.MTT실험중수착계안염각취당농도적증고(분별위50,100,200 μg/mL)대A549세포생장적억제솔적축점증강[분별위(27.57±0.037)％,(66.69±0.40)％,(81.55±0.72)％],MMP-9단백표체수평야수지강저,정제량의뢰성,실험조여대조조비교유차이유통계학의의(P＜0.05).결론 계안염각취당능구억제인비소세포폐암A549세포적증식,감소MMP-9적표체,진이강저종류세포적침습、전이능력,기작용효응정제량의뢰성.
Objective To investigate the effects of Hydroxypropyltrimethyl ammonium chloride chitosan(HACC) on the proliferation and invasion capacity of A549 cells.Methods A549 cells were cultured in RPMI-1640 medium with different concentrations of HACC.Trypan blue stain and MTT assay were performed to detect the cytoactive and inhibitory ratio of A549 proliferation,respectively.Mo~over,the expression of matrix metalloproteinase 9 (MMP-9) was detected by Western blot.Results The trypan blue stain results indicated that the survival rate of A549 cells was more than 95％,which was used in the following experiments.The inhibitory ratio of A549 proliferation was increased [(27.57 ± 0.37) ％,(66.96 ± 0.40) ％,(81.55 ± 0.72) ％] and the expression of MMP-9 was decreased as the concentration of HACC in RPM-1640 Medium(50,100,200 μg/mL) was increasing.HACC exerted the inhibitory effect on cell proliferation and the expression of MMP-9 in a dose dependent mamer(P ＜ 0.05).Conclusion HACC inhibits the proliferation of A549 cells and its MMP-9 expression,which results in the restrain of its invasion and metastasis.
