CAJ | 학술논문

目的探讨移植器官体积对移植器官生存时间的影响.方法 C57BL/6小鼠与BALB/c小鼠杂交获得F1小鼠,1.5×107个F1小鼠脾脏细胞输注给新生C57BL/6小鼠;混合淋巴细胞反应检测体内同种异体反应性T细胞的克隆清除;Na(i)ve(初始)C57BL/6小鼠和新生期处理的C57BL/6小鼠移植F1小鼠皮肤,皮片大小分别为0.5 cm2和1 cm2,观察皮片的生存时间.结果新生期C57BL/6小鼠输注F1小鼠脾脏细胞后体内同种异体反应性T细胞数量显著减少,对移植抗原的反应性减弱;Na(i)ve小鼠迅速排斥F1小鼠皮肤移植物,0.5 cm2和1 cm2组皮肤移植物的生存时间分别为(8.9±1.0)d和(9.3±1.0)d,两组间差异没有统计学意义(P＞0.05);新生期处理的C57BL/6小鼠移植F1小鼠皮肤,1 cm2组皮肤移植物生存时间为(63±28)d,大于0.5 cm2组皮肤移植物(28±16)d,两组间差异具有统计学意义(P＜0.05).0.5 cm2组皮肤移植物在60d内完全排斥,1 cm2组皮肤移植物在100 d时仍有部分存活,形成长期耐受.结论降低移植受体排斥强度的同时增加移植器官的体积可延长移植器官的生存时间.
목적 탐토이식기관체적대이식기관생존시간적영향.방법 C57BL/6소서여BALB/c소서잡교획득F1소서,1.5×107개F1소서비장세포수주급신생C57BL/6소서;혼합림파세포반응검측체내동충이체반응성T세포적극륭청제;Na(i)ve(초시)C57BL/6소서화신생기처리적C57BL/6소서이식F1소서피부,피편대소분별위0.5 cm2화1 cm2,관찰피편적생존시간.결과 신생기C57BL/6소서수주F1소서비장세포후체내동충이체반응성T세포수량현저감소,대이식항원적반응성감약;Na(i)ve소서신속배척F1소서피부이식물,0.5 cm2화1 cm2조피부이식물적생존시간분별위(8.9±1.0)d화(9.3±1.0)d,량조간차이몰유통계학의의(P＞0.05);신생기처리적C57BL/6소서이식F1소서피부,1 cm2조피부이식물생존시간위(63±28)d,대우0.5 cm2조피부이식물(28±16)d,량조간차이구유통계학의의(P＜0.05).0.5 cm2조피부이식물재60d내완전배척,1 cm2조피부이식물재100 d시잉유부분존활,형성장기내수.결론 강저이식수체배척강도적동시증가이식기관적체적가연장이식기관적생존시간.
Objective To investigate the effects of graft size on the survival of the graft.Methods F1 mice were bred by crossing male C57BL/6 and female BALB/c mice.Newborn C57BL/6 mice were infused with 1.5 × 107 F1 spleen cells(newborn-induced transplant tolerance,NTT).Clonal deletion of alloreactive T cells was measured by mixed lymphocyte reaction (MLR).Na(i)ve and NTT mice were transplanted with 0.5 cm2 and 1 cm2 skin grafts from F1 mice to evaluate their tolerance.Results Infusion of spleen cells from F1 mice significantly depleted alloreactive T cells from C57BL/6 mice,and reduced anti-graft reactivity.Naive mice rapidly rejected both 0.5 cm2 and 1 cm2 skin grafts with same process [(8.9 ± 1.0) days and (9.3 ± 1.0) days,P ＞ 0.05].The survival time of 1 cm2 skin grafts in NTT mice is higher than that of 0.5 cm2 skin grafts with (63 ± 28) vs (28 ± 16) days respectively (P ＜ 0.05).Long-term tolerance was observed in 1 cm2 but not in 0.5 cm2 skin grafts.Conclusion Enlarging the volume of the graft in conditions with reduced frequency of alloreactive T cells can prolong graft survival time.