CAJ | 학술논문

目的检测缺血性脑血管病患者服用他汀类药物前后外周血中与体液免疫相关的指标(CD19+ CD27+细胞、IL-21、总IgG)的变化,初步探究他汀类药物在缺血性脑血管病患者中对B细胞的免疫调节作用.方法采用流式细胞仪检测11名缺血性脑血管病患者接受他汀类药物治疗前后外周血中记忆B细胞(CD19+CD27+细胞)的百分比,分别用酶联免疫吸附试验(ELISA)和免疫比浊法检测17名缺血性脑血管病患者服用他汀类药物前后血清中IL-21和IgG水平,对检测结果进行服药前后自身对照,使用配对t-检验进行统计分析.结果流式检测显示缺血性脑血管病患者服用他汀类药物后外周血中记忆B细胞(CD19+ CD27+细胞)数量下降[(3.39±1.77)％比(2.36±1.29)％,P=0.019],血清中IL-21水平下降[(112.79 ±45.19)比(88.83±18.40) pg/mL,P=0.024],血清中总IgG水平升高[(10.74±2.41)比(11.97 ±2.61) g/L,P=0.03].结论他汀类药物对体液免疫有调节作用.
목적 검측결혈성뇌혈관병환자복용타정류약물전후외주혈중여체액면역상관적지표(CD19+ CD27+세포、IL-21、총IgG)적변화,초보탐구타정류약물재결혈성뇌혈관병환자중대B세포적면역조절작용.방법 채용류식세포의검측11명결혈성뇌혈관병환자접수타정류약물치료전후외주혈중기억B세포(CD19+CD27+세포)적백분비,분별용매련면역흡부시험(ELISA)화면역비탁법검측17명결혈성뇌혈관병환자복용타정류약물전후혈청중IL-21화IgG수평,대검측결과진행복약전후자신대조,사용배대t-검험진행통계분석.결과 류식검측현시결혈성뇌혈관병환자복용타정류약물후외주혈중기억B세포(CD19+ CD27+세포)수량하강[(3.39±1.77)％비(2.36±1.29)％,P=0.019],혈청중IL-21수평하강[(112.79 ±45.19)비(88.83±18.40) pg/mL,P=0.024],혈청중총IgG수평승고[(10.74±2.41)비(11.97 ±2.61) g/L,P=0.03].결론 타정류약물대체액면역유조절작용.
Objective To explore the effects of statin on the CD19 + CD27 + cells,IL-21 and serum total IgG of the patients with cerebrovascular diseases.Methods CD19 + CD27 + cells (memory B cells),CD19 + CD27 cells,and CD19 + cells in the peripheral blood of the patients with ischemis cerebrovascular diseases were measured by flow cytometry.The levels of IL-21 and total IgG in serum were measured by enzyme linked immunosorbent essay(ELISA) and immunoturbidimetric assays respectively before and after statin therapy.Paired t-test was used to compare the results from the same subjects before and after treatment.Results Statin treatment decreased the percentages of CD19 + CD27 + cells [(3.29 ± 1.27) ％ vs (2.36 ± 1.29) ％,P =0.019] and the serum level of IL-21 [(112.79 ± 45.19) vs (88.83 ± 18.40) pg/mL,P =0.024],but it increased total serum IgG level[(10.74 ± 2.41) vs (11.97 ± 2.61) g/L,P =0.03].Conclusion Administration of statins may regulate humoral immunity in vivo.