国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2014年
6期
531-535,539
,共6页
柴琳%詹渊博%宋雪静%徐晓满%穆森%张瑞敏
柴琳%詹淵博%宋雪靜%徐曉滿%穆森%張瑞敏
시림%첨연박%송설정%서효만%목삼%장서민
慢性牙周炎%牙周基础治疗%非刺激性全唾液%基质金属蛋白酶-9
慢性牙週炎%牙週基礎治療%非刺激性全唾液%基質金屬蛋白酶-9
만성아주염%아주기출치료%비자격성전타액%기질금속단백매-9
Chronic periodontitis%Periodontal initial therapy%Unstimulated whole saliva%Matrix metalloproteinases-9
目的 评估慢性牙周炎患者经牙周基础治疗前后的非刺激性全唾液、龈沟液及血清中基质金属蛋白酶-9 (MMp-9)水平,探讨其作为牙周炎诊断及预后标志物的可能性.方法 酶联免疫吸附试验(ELISA)检测20名牙周炎患者治疗前、后及20名健康人非刺激性全唾液、龈沟液及血清中MMP-9的水平,并记录牙周袋探诊深度(PD)、临床附着丧失(CAL)和出血指数(BI).结果 ①除CAL外,经牙周基础治疗6周后慢性牙周炎患者的临床指标明显改善[CAL治疗前(5.50±1.97) mm,治疗后(5.50±1.97) mm,P=1.000;PD治疗前(7.05±1.81) mm,治疗后(4.23±1.06) mm,P=0.000;BI治疗前3.75±0.44,治疗后0.20±0.41,P=0.000);②治疗后非刺激性全唾液、龈沟液及血清中MMP-9水平明显降低,与治疗前相比差异具有统计学意义[非刺激性全唾液MMP-9水平治疗前(22.89±5.28)ng/mL,治疗后(18.11±7.19) ng/mL,P=0.003;龈沟液MMP-9水平治疗前(41.80±2.90) ng/mL,治疗后(35.71±4.49) ng/mL,P=0.000;血清MMP-9水平治疗前(6.67±5.318) ng/mL,治疗后(2.47±2.713) ng/mL,P=0.004];③除血清外,牙周炎患者治疗前后的非刺激性全唾液和龈沟液中MMP-9水平仍高于健康对照组,其差异有统计学意义[非刺激性全唾液水平为(6.78±3.02)ng/mL,龈沟液为(30.20±3.64) ng/mL,与治疗前后比较P=0.000;健康对照组血清MMP-9水平(1.18±0.88) ng/mL,与治疗前、后比较P =0.004、P=0.055].结论 非刺激性全唾液中MMP-9的表达水平可能成为慢性牙周炎的检测指标之一,可能为临床诊治提供参考依据.
目的 評估慢性牙週炎患者經牙週基礎治療前後的非刺激性全唾液、齦溝液及血清中基質金屬蛋白酶-9 (MMp-9)水平,探討其作為牙週炎診斷及預後標誌物的可能性.方法 酶聯免疫吸附試驗(ELISA)檢測20名牙週炎患者治療前、後及20名健康人非刺激性全唾液、齦溝液及血清中MMP-9的水平,併記錄牙週袋探診深度(PD)、臨床附著喪失(CAL)和齣血指數(BI).結果 ①除CAL外,經牙週基礎治療6週後慢性牙週炎患者的臨床指標明顯改善[CAL治療前(5.50±1.97) mm,治療後(5.50±1.97) mm,P=1.000;PD治療前(7.05±1.81) mm,治療後(4.23±1.06) mm,P=0.000;BI治療前3.75±0.44,治療後0.20±0.41,P=0.000);②治療後非刺激性全唾液、齦溝液及血清中MMP-9水平明顯降低,與治療前相比差異具有統計學意義[非刺激性全唾液MMP-9水平治療前(22.89±5.28)ng/mL,治療後(18.11±7.19) ng/mL,P=0.003;齦溝液MMP-9水平治療前(41.80±2.90) ng/mL,治療後(35.71±4.49) ng/mL,P=0.000;血清MMP-9水平治療前(6.67±5.318) ng/mL,治療後(2.47±2.713) ng/mL,P=0.004];③除血清外,牙週炎患者治療前後的非刺激性全唾液和齦溝液中MMP-9水平仍高于健康對照組,其差異有統計學意義[非刺激性全唾液水平為(6.78±3.02)ng/mL,齦溝液為(30.20±3.64) ng/mL,與治療前後比較P=0.000;健康對照組血清MMP-9水平(1.18±0.88) ng/mL,與治療前、後比較P =0.004、P=0.055].結論 非刺激性全唾液中MMP-9的錶達水平可能成為慢性牙週炎的檢測指標之一,可能為臨床診治提供參攷依據.
목적 평고만성아주염환자경아주기출치료전후적비자격성전타액、간구액급혈청중기질금속단백매-9 (MMp-9)수평,탐토기작위아주염진단급예후표지물적가능성.방법 매련면역흡부시험(ELISA)검측20명아주염환자치료전、후급20명건강인비자격성전타액、간구액급혈청중MMP-9적수평,병기록아주대탐진심도(PD)、림상부착상실(CAL)화출혈지수(BI).결과 ①제CAL외,경아주기출치료6주후만성아주염환자적림상지표명현개선[CAL치료전(5.50±1.97) mm,치료후(5.50±1.97) mm,P=1.000;PD치료전(7.05±1.81) mm,치료후(4.23±1.06) mm,P=0.000;BI치료전3.75±0.44,치료후0.20±0.41,P=0.000);②치료후비자격성전타액、간구액급혈청중MMP-9수평명현강저,여치료전상비차이구유통계학의의[비자격성전타액MMP-9수평치료전(22.89±5.28)ng/mL,치료후(18.11±7.19) ng/mL,P=0.003;간구액MMP-9수평치료전(41.80±2.90) ng/mL,치료후(35.71±4.49) ng/mL,P=0.000;혈청MMP-9수평치료전(6.67±5.318) ng/mL,치료후(2.47±2.713) ng/mL,P=0.004];③제혈청외,아주염환자치료전후적비자격성전타액화간구액중MMP-9수평잉고우건강대조조,기차이유통계학의의[비자격성전타액수평위(6.78±3.02)ng/mL,간구액위(30.20±3.64) ng/mL,여치료전후비교P=0.000;건강대조조혈청MMP-9수평(1.18±0.88) ng/mL,여치료전、후비교P =0.004、P=0.055].결론 비자격성전타액중MMP-9적표체수평가능성위만성아주염적검측지표지일,가능위림상진치제공삼고의거.
Objective To evaluate the MMP-9 levels in the unstimulated whole saliva (UWS),gingival crevicular fluid (GCF) and serum of patients with chronic periodontitis before and after the initial phase of periodontal treatment and to determine whether they are possible diagnostic and prognostic biomarkers for periodontitis.Methods Twenty patients with periodontitis and 20 healthy controls were included,and periodontal conditions (BI,PD,CAL) were assessed.The levels of MMP-9 in UWS,GCF and serum were measured by enzyme-linked immunosorbent assay before and after the treatment (BT and AT).Results ① In addition to CAL,the conventional periodontal treatment resulted in significant improvement in clinical parameters compared with before treatment.[CAL BT(5.50 ± 1.97) mm,AT (5.50 ± 1.97) mm,P =1.000 ; PD BT (7.05 ± 1.81) mm,AT(4.23 ± 1.06)mm,P =0.000;BI BT 3.75 ±0.44,AT 0.20 ±0.41,P =0.000]②MMP-9 levels in UWS,GCF and serum were significantly decreased six weeks after treatment[UWS BT(22.89 ± 5.28)ng/mL,AT(18.11 ±7.19) ng/mL,P =0.000;GCF BT(41.80 ±2.90) ng/mL,AT(35.71 ±4.49) ng/mL,P =0.000;serum BT(6.67 ± 5.318) ng/mL,AT (2.47 ± 2.713) ng/mL,P =0.004] ③In addition to serum,MMP-9 levels in UWS and GCF before and after the initial phase of periodontal treatment revealed significant increases compared with the control group[UWS of control group (6.78 ± 3.02) ng/mL,GCF (30.20 ± 3.64) ng/mL,P =0.000 ; serum of control group (1.18 ± 0.88) ng/mL,compared with BT and AT P =0.004,P =0.055].Conclusions The high expression of MMP-9 in unstimulated whole saliva is likely to become indicators in chronic periodontitis,and may provide a reference for clinical diagnosis and treatment.
