国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2014年
6期
536-539
,共4页
杨博%高宇巍%杨澄%李宏伟
楊博%高宇巍%楊澄%李宏偉
양박%고우외%양징%리굉위
C形臂X光机%氧化水平%免疫水平%造血系统
C形臂X光機%氧化水平%免疫水平%造血繫統
C형비X광궤%양화수평%면역수평%조혈계통
C-arm X ray%Oxidation level%Immune level%Hemopoietic system
目的 探讨研究C形臂X光机急性扫描对小鼠机体功能的影响.方法 健康雄性BALB/c小鼠60只随机分为对照组、单次、四次、八次和十二次扫描组,每组12只.对照组小鼠不扫描,其余组用C形臂X光机对小鼠进行全身扫描.在照射后24小时,采用生物化学方法检测小鼠甲状腺组织中超氧化物歧化酶(SOD)和丙二醛(MDA)的水平;采用酶联免疫吸附试验(ELISA)检测血清中IL-4和IFN-γ的变化;流式细胞仪检测各照射组小鼠外周血淋巴细胞和骨髓细胞的凋亡水平.结果 与对照组比较,小鼠甲状腺组织中的MDA水平在八次以上扫描后分别升高了2.39、3.49倍(P＜0.05),而SOD水平在四次以上扫描后分别降低了19.23％、25.80％和27.95％ (P ＜0.05);血清IFN-γ和IL-4水平在四次以上扫描后分别降低了14.71％和22.60％、22.99％和44.05％、31.70％和44.43％ (P＜0.05),但均未影响IFN-γ/IL-4值(P＞0.05);小鼠外周血淋巴细胞的凋亡水平在八次以上扫描后分别增加了1.68、2.40倍(P＜0.05),骨髓细胞的凋亡水平在十二次扫描后增加了2.02倍(P＜0.05).结论 C形臂X光机多次扫描改变了机体的氧化水平和免疫学相关指标.另外,机体通过启动凋亡来维持基因组稳定性.
目的 探討研究C形臂X光機急性掃描對小鼠機體功能的影響.方法 健康雄性BALB/c小鼠60隻隨機分為對照組、單次、四次、八次和十二次掃描組,每組12隻.對照組小鼠不掃描,其餘組用C形臂X光機對小鼠進行全身掃描.在照射後24小時,採用生物化學方法檢測小鼠甲狀腺組織中超氧化物歧化酶(SOD)和丙二醛(MDA)的水平;採用酶聯免疫吸附試驗(ELISA)檢測血清中IL-4和IFN-γ的變化;流式細胞儀檢測各照射組小鼠外週血淋巴細胞和骨髓細胞的凋亡水平.結果 與對照組比較,小鼠甲狀腺組織中的MDA水平在八次以上掃描後分彆升高瞭2.39、3.49倍(P＜0.05),而SOD水平在四次以上掃描後分彆降低瞭19.23％、25.80％和27.95％ (P ＜0.05);血清IFN-γ和IL-4水平在四次以上掃描後分彆降低瞭14.71％和22.60％、22.99％和44.05％、31.70％和44.43％ (P＜0.05),但均未影響IFN-γ/IL-4值(P＞0.05);小鼠外週血淋巴細胞的凋亡水平在八次以上掃描後分彆增加瞭1.68、2.40倍(P＜0.05),骨髓細胞的凋亡水平在十二次掃描後增加瞭2.02倍(P＜0.05).結論 C形臂X光機多次掃描改變瞭機體的氧化水平和免疫學相關指標.另外,機體通過啟動凋亡來維持基因組穩定性.
목적 탐토연구C형비X광궤급성소묘대소서궤체공능적영향.방법 건강웅성BALB/c소서60지수궤분위대조조、단차、사차、팔차화십이차소묘조,매조12지.대조조소서불소묘,기여조용C형비X광궤대소서진행전신소묘.재조사후24소시,채용생물화학방법검측소서갑상선조직중초양화물기화매(SOD)화병이철(MDA)적수평;채용매련면역흡부시험(ELISA)검측혈청중IL-4화IFN-γ적변화;류식세포의검측각조사조소서외주혈림파세포화골수세포적조망수평.결과 여대조조비교,소서갑상선조직중적MDA수평재팔차이상소묘후분별승고료2.39、3.49배(P＜0.05),이SOD수평재사차이상소묘후분별강저료19.23％、25.80％화27.95％ (P ＜0.05);혈청IFN-γ화IL-4수평재사차이상소묘후분별강저료14.71％화22.60％、22.99％화44.05％、31.70％화44.43％ (P＜0.05),단균미영향IFN-γ/IL-4치(P＞0.05);소서외주혈림파세포적조망수평재팔차이상소묘후분별증가료1.68、2.40배(P＜0.05),골수세포적조망수평재십이차소묘후증가료2.02배(P＜0.05).결론 C형비X광궤다차소묘개변료궤체적양화수평화면역학상관지표.령외,궤체통과계동조망래유지기인조은정성.
Objective To investigate the biological effects induced by C-arm X ray acute scan in mice.Methods 60 healthy male BALB/c mice were randomly divided into control group,single,four,eight and twelve times of C-arm X ray scan; there were 12 mice in each group.The mice were administered with different frequency of whole-body C-arm X ray scan; After 24 h of scan,malondialdehyde (MDA) and superoxide dismutase(SOD) were detected;Enzyme Linked Immuno Sorbent Assay (ELISA) was used to detect the level of IL-4 and IFN-γin serum.The apoptosis level of peripheral lymphocytes and bone marrow cells was analyzed by flow cytometry.Results Compared with control,more than eight times of scan increased MDA level by 2.39 and 3.49,but more than four times of scan decreased SOD level in thyroid by 19.23％,25.80％ and 27.95％.More than four times of scan decreased the level of IFN-γ and IL-4 in the serum by 14.71％ and 22.60％,22.99％ and 44.05％,31.70％ and 44.43％,but no influence on the ratio of IFN-γ/IL-4 was observed.More than eight and twelve times of scan increased apoptosis level in peripheral lymphocytes and bone marrow cells by 1.68,2.40 and 2.02.Conclusion Whith Multiple scan C-arm X ray changed oxidative level and immunological response.In addition,apoptosis was initiated to maintain genome stability.
