CAJ | 학술논문

目的利用已建立的细胞-细胞融合实验体系,扩大毒株范围,通过观察凝血酶(Th)对Ⅰ型人类免疫缺陷病毒(HIV)-1包膜V3区冠部序列不同的毒株介导的细胞融合的影响及其原因.方法采用携带HIV-1 ADA、06057c3、03009c34株env基因的表达质粒pSVⅢ-ADA、pSM-06057c3、pSM-03009c34与pcTat分别按2∶1的比例共转染293T细胞,制备Env-293T细胞,用不同浓度的Th处理,与Magic5A细胞进行融合实验,计融合细胞数,观察对融合作用的影响.结果 Th能增强ADA和06057c3Env介导的融合,它们的V3冠部的序列为GPGRAF,但不能增强V3冠部序列为GPGQAW的毒株03009c34 Env介导的融合.结论 Th能促进HIV介导的细胞融合,其原因可能是依赖V3冠部GPGRAF序列.
목적 이용이건립적세포-세포융합실험체계,확대독주범위,통과관찰응혈매(Th)대Ⅰ형인류면역결함병독(HIV)-1포막V3구관부서렬불동적독주개도적세포융합적영향급기원인.방법 채용휴대HIV-1 ADA、06057c3、03009c34주env기인적표체질립pSVⅢ-ADA、pSM-06057c3、pSM-03009c34여pcTat분별안2∶1적비례공전염293T세포,제비Env-293T세포,용불동농도적Th처리,여Magic5A세포진행융합실험,계융합세포수,관찰대융합작용적영향.결과 Th능증강ADA화06057c3Env개도적융합,타문적V3관부적서렬위GPGRAF,단불능증강V3관부서렬위GPGQAW적독주03009c34 Env개도적융합.결론 Th능촉진HIV개도적세포융합,기원인가능시의뢰V3관부GPGRAF서렬.
Objective To observe the effects and reasons of thrombin on HIV envelope V3 area crown sequence in different strain by using cell-cell fusion experimental system and expend the range of strain.Methods In order to prepare theEnv-293T cell by transfecting 293T cells,plasmid pSVⅢ-ADA,plasmid pSM-06057c3 and plasmid pSM-03009c34 are selected.And these three kinds of plasmids must carry HIV-1 ADA,06057c3 and 03009c34 env genes.239T cell are transfected by plasmid pSVⅢ-ADA and pcTat,pSM-06057c3 and pcTat pSM-03009c34 and pcTat.The ratio of plamid and pcTat is 2∶ 1.After that there is a cell fusion experiment between the Env-293T cell and Magic5A cell in different Th concentrations and count the fusion cells and observe the effect of cell fusion.Results The treated cell can promote ADA and 06057c3 Env mediated fusion,whose V3 crown sequences are GPGRAF.Th can not promote 03009c34 Env mediated fusion,whose V3 crown sequence is GPGQAW strain.Conclusion Th can promote HIV meditated cell fusion,and the reason which might be related with the V3 crown GPGRAF sequences.