CAJ | 학술논문

目的探讨氯胺酮对大鼠海马神经元低电压激活钙电流[low-voltage-activated calcium currents,Ⅰca(LvA)]的影响.方法原代培养Wistar大鼠海马神经元,采用全细胞膜片钳技术测定ⅠCa(LVA).记录不同浓度(10、30、100、300、1 000 μmol/L)氯胺酮下ⅠCa(LVA)的峰电流密度,计算ⅠCa(LVA)抑制率,并绘制氯胺酮的浓度效应曲线,计算半数抑制浓度(IC50)和Hill系数.并记录100 μmol/L氯胺酮对Ⅰca (LVA)激活和失活动力学的影响. 结果氯胺酮10 μmol/L给药前、后ⅠCa(LVA)峰电流密度差异无统计学意义(P＞0.05)；30、100、300、1 000 μmol/L氯胺酮对ⅠCa(LVA)峰电流密度抑制率分别为(12±4)％、(19±4)％、(32±3)％、(31±2)％.与给药前比较,氯胺酮呈浓度依赖性地抑制Ⅰca(LVA)峰电流密度(P＜0.05).IC50为110 μmol/L,Hill系数为0.14.给药前、后半数最大激活膜电位分别为(-8±0)mV和(-10±2)mV,斜率因子κ分别为(7±0)和(8±1)；半数最大失活电压为(-42±6) mV和(-48±6) mV,κ分别为(22±5)和(27±7),给药前、后比较,差异均无统计学意义(P＞0.05). 结论氯胺酮可显著抑制大鼠海马神经元ⅠCa(LVA),氯胺酮对中枢神经系统的作用可能与抑制ⅠCa (LVA)有关.
목적 탐토록알동대대서해마신경원저전압격활개전류[low-voltage-activated calcium currents,Ⅰca(LvA)]적영향.방법 원대배양Wistar대서해마신경원,채용전세포막편겸기술측정ⅠCa(LVA).기록불동농도(10、30、100、300、1 000 μmol/L)록알동하ⅠCa(LVA)적봉전류밀도,계산ⅠCa(LVA)억제솔,병회제록알동적농도효응곡선,계산반수억제농도(IC50)화Hill계수.병기록100 μmol/L록알동대Ⅰca (LVA)격활화실활동역학적영향. 결과 록알동10 μmol/L급약전、후ⅠCa(LVA)봉전류밀도차이무통계학의의(P＞0.05)；30、100、300、1 000 μmol/L록알동대ⅠCa(LVA)봉전류밀도억제솔분별위(12±4)％、(19±4)％、(32±3)％、(31±2)％.여급약전비교,록알동정농도의뢰성지억제Ⅰca(LVA)봉전류밀도(P＜0.05).IC50위110 μmol/L,Hill계수위0.14.급약전、후반수최대격활막전위분별위(-8±0)mV화(-10±2)mV,사솔인자κ분별위(7±0)화(8±1)；반수최대실활전압위(-42±6) mV화(-48±6) mV,κ분별위(22±5)화(27±7),급약전、후비교,차이균무통계학의의(P＞0.05). 결론 록알동가현저억제대서해마신경원ⅠCa(LVA),록알동대중추신경계통적작용가능여억제ⅠCa (LVA)유관.
Objective To investigate the effect of ketamine on the low-voltage-activated calcium currents [ⅠCa (LVA)]in rat hippocampal neurons.Methods Hippocampal neurons were isolated from Wistar rats and cultured.ⅠCa (LVA) was recorded using whole-cell patch clamp technique.Different concentrations of ketamine were added to the culture.The effect of ketamine on ⅠCa(LVA)was evaluated.Results ⅠCa (LVA) was inhibited by ketamine in a concentration-dependent manner.Ketamine at the concentration of 10 μmol/L had no effect on ⅠCa (LVA)· Ketamine at the concentration of 30,100,300 μmol/L and 1 000 μmol/L reduced peak ⅠCa (LVA)by (12±4)％,(19±4)％,(32±3)％,(31±2)％ respectively.The IC50 was at 110 μmol/L and Hill coefficient was 0.14.V1/2 of activation curve was shifted from (-8±0) mV to (-10±2) mV.The V1/2 of inactivation curve was shifted from (-42±6) mV to (-48±6) mV.Conclusions Ketamine produces significant inhibition of ICa (LVA) in hippocampal neurons which may partly explain the action of ketamine on central nervous system.