国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2013年
7期
591-594,604
,共5页
刘献文%逯素芬%于爱兰%张宗旺
劉獻文%逯素芬%于愛蘭%張宗旺
류헌문%록소분%우애란%장종왕
尼古丁%美加明%依赖%戒断%疼痛
尼古丁%美加明%依賴%戒斷%疼痛
니고정%미가명%의뢰%계단%동통
Nicotine%Mecamylamine%Dependence%Withdrawal%Pain
目的 通过间断皮下注射尼古丁制作改良尼古丁依赖-戒断大鼠模型,观察尼古丁戒断后大鼠机械刺激缩足阈值(mechanical withdrawal threshold,MWT)和热刺激缩足潜伏期(thermal withdrawal latency,TWL)的变化. 方法 SD大鼠30只,采用随机数字表法随机分成5组,每组6只:正常对照组(Control组)、生理盐水组(NS组)、尼古丁3 mg· kg-1·d-1组(NT3组)、尼古丁9 mg·kg-1·d-1组(NT9组)和尼古丁18 mg· kg-1· d1组(NT18组),分别不注射,皮下注射生理盐水、尼古丁1 mg/kg、3 mg/kg和6 mg/kg,3次/d,连续7d.末次注射后60 min皮下注射美加明1 mg/kg.观察大鼠注射尼古丁期间和戒断后体重变化、存活情况和尼古丁戒断评分.另外选取Control组、NS组和NT9组各6只大鼠,测定右下肢足底MWT和TWL. 结果 与NT3组比较,注射尼古丁后第7天,NT9组和NT18组大鼠体重增加缓慢[(3.8±1.3)、(2.0±0.3)g vs (7.2±1.0)g](P＜0.05),戒断后第1天和第2天大鼠体重增加迅速(P＜0.01).NT9组和NT18组大鼠美加明激发后出现更多的戒断症状(P＜0.01),NT18组大鼠死亡率为17％.与Control组比较,NT9组大鼠在尼古丁戒断后MWT与TWL明显降低(P＜0.01). 结论 间断皮下注射尼古丁9 mg·kg-1·d-17 d可以成功制作改良尼古丁依赖-戒断大鼠模型,尼古丁戒断后大鼠疼痛敏感性增高.
目的 通過間斷皮下註射尼古丁製作改良尼古丁依賴-戒斷大鼠模型,觀察尼古丁戒斷後大鼠機械刺激縮足閾值(mechanical withdrawal threshold,MWT)和熱刺激縮足潛伏期(thermal withdrawal latency,TWL)的變化. 方法 SD大鼠30隻,採用隨機數字錶法隨機分成5組,每組6隻:正常對照組(Control組)、生理鹽水組(NS組)、尼古丁3 mg· kg-1·d-1組(NT3組)、尼古丁9 mg·kg-1·d-1組(NT9組)和尼古丁18 mg· kg-1· d1組(NT18組),分彆不註射,皮下註射生理鹽水、尼古丁1 mg/kg、3 mg/kg和6 mg/kg,3次/d,連續7d.末次註射後60 min皮下註射美加明1 mg/kg.觀察大鼠註射尼古丁期間和戒斷後體重變化、存活情況和尼古丁戒斷評分.另外選取Control組、NS組和NT9組各6隻大鼠,測定右下肢足底MWT和TWL. 結果 與NT3組比較,註射尼古丁後第7天,NT9組和NT18組大鼠體重增加緩慢[(3.8±1.3)、(2.0±0.3)g vs (7.2±1.0)g](P＜0.05),戒斷後第1天和第2天大鼠體重增加迅速(P＜0.01).NT9組和NT18組大鼠美加明激髮後齣現更多的戒斷癥狀(P＜0.01),NT18組大鼠死亡率為17％.與Control組比較,NT9組大鼠在尼古丁戒斷後MWT與TWL明顯降低(P＜0.01). 結論 間斷皮下註射尼古丁9 mg·kg-1·d-17 d可以成功製作改良尼古丁依賴-戒斷大鼠模型,尼古丁戒斷後大鼠疼痛敏感性增高.
목적 통과간단피하주사니고정제작개량니고정의뢰-계단대서모형,관찰니고정계단후대서궤계자격축족역치(mechanical withdrawal threshold,MWT)화열자격축족잠복기(thermal withdrawal latency,TWL)적변화. 방법 SD대서30지,채용수궤수자표법수궤분성5조,매조6지:정상대조조(Control조)、생리염수조(NS조)、니고정3 mg· kg-1·d-1조(NT3조)、니고정9 mg·kg-1·d-1조(NT9조)화니고정18 mg· kg-1· d1조(NT18조),분별불주사,피하주사생리염수、니고정1 mg/kg、3 mg/kg화6 mg/kg,3차/d,련속7d.말차주사후60 min피하주사미가명1 mg/kg.관찰대서주사니고정기간화계단후체중변화、존활정황화니고정계단평분.령외선취Control조、NS조화NT9조각6지대서,측정우하지족저MWT화TWL. 결과 여NT3조비교,주사니고정후제7천,NT9조화NT18조대서체중증가완만[(3.8±1.3)、(2.0±0.3)g vs (7.2±1.0)g](P＜0.05),계단후제1천화제2천대서체중증가신속(P＜0.01).NT9조화NT18조대서미가명격발후출현경다적계단증상(P＜0.01),NT18조대서사망솔위17％.여Control조비교,NT9조대서재니고정계단후MWT여TWL명현강저(P＜0.01). 결론 간단피하주사니고정9 mg·kg-1·d-17 d가이성공제작개량니고정의뢰-계단대서모형,니고정계단후대서동통민감성증고.
Objective To establish a modified rat model of nicotine dependence-withdrawal syndrome by subcutaneous injection of nicotine,and observe mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) after nicotine withdrawal.Methods Thirty SD rats,were randomly divided into 5 groups with 6 rats per group,including control group,normal saline group (NS group),nicotine 3 mg·kg1·d-1 group (NT3 group),nicotine 9 mg·kg-1·d-1 group (NT9 group) and nicotine 18 mg·kg-1·d-1group (NT18 group),received no injection,subcutaneous injection of normal saline,nicotine 1,3 mg/kg and 6 mg/kg respectively three times a day for seven days.At 60 min after the last injection of nicotine,subcutaneous injection of mecamylamine 1 mg/kg was given for each rat.Weight changes of rat during injections of nicotine and after nicotine withdrawal,survival rate and its withdrawal symptoms score were recorded.In addition,control group,NS group and NT9 group with 6 rats in each group were selected for the determination of MWT and TWL in the right hind paw during nicotine withdrawal.Results Compared with NT3 group,on the seventh day after injection of nicotine,weight gain of the rats was significantly slow in NT9 group and NT18 group [(3.8±1.3),(2.0±0.3) g vs (7.2±1.0) g](P＜0.05).On the first and second withdrawal days,weight gain of the rats was significantly rapid in NT9 group and NT18 group (P＜0.01).Compared with NT3 group,the rats in NT9 group and NT18 group had significant more withdrawal symptoms after mecamylamine injection (P＜0.01).The incidence of mortality in NT18 group was 17％.MWT and TWL decreased significantly after nicotine withdrawal in NT9 group than control group (P＜0.01).Conclusions A modified rat model of nicotine dependence-withdrawal syndrome can be successfully established by discontinuous subcutaneous injection of nicotine 9 mg ·kg-1 ·d-1 for seven days.The pain sensitivity increases in rats with nicotine withdrawal.
