CAJ | 학술논문

目的探讨阿霉素(ADM)干预下人肾上腺皮质癌裸鼠模型干细胞标志CD133、ABCG2的表达及意义.方法 SW-13细胞系建立裸鼠皮下移植瘤模型,ADM体内干预后原代培养获得ADM组细胞株,未予干预直接原代培养获得对照组细胞株.用单细胞成克隆技术检测细胞的增殖能力,免疫组化法和流式细胞法检测肿瘤干细胞标志物CD133、ABCG2的表达.结果 ADM组细胞株的克隆形成率高于对照组(P＜0.05)；流式细胞法和免疫组化法提示ABCG2在两组细胞中均有阳性表达,ADM组细胞株的流式细胞表达率高于对照组细胞株(P＜0.05)；以上两种方法均表明CD133在两组中阴性表达.结论 ADM组细胞具有一定的肿瘤干细胞特征,ABCG2参与肾上腺皮质癌细胞耐药机制,可能为分子靶向治疗的潜在靶点.
목적 탐토아매소(ADM)간예하인신상선피질암라서모형간세포표지CD133、ABCG2적표체급의의.방법 SW-13세포계건립라서피하이식류모형,ADM체내간예후원대배양획득ADM조세포주,미여간예직접원대배양획득대조조세포주.용단세포성극륭기술검측세포적증식능력,면역조화법화류식세포법검측종류간세포표지물CD133、ABCG2적표체.결과 ADM조세포주적극륭형성솔고우대조조(P＜0.05)；류식세포법화면역조화법제시ABCG2재량조세포중균유양성표체,ADM조세포주적류식세포표체솔고우대조조세포주(P＜0.05)；이상량충방법균표명CD133재량조중음성표체.결론 ADM조세포구유일정적종류간세포특정,ABCG2삼여신상선피질암세포내약궤제,가능위분자파향치료적잠재파점.
Objective To explore the expression of CD133 and ABCG2 in adrenocortical carcinoma (ACC) xenograft treated by adriamycin in vivo,and to preliminarily analyze the significance.Methods Established the nude mice model of SW-13 transplanted subcutaneously,one group was intervened with ADM in vivo and obtained ADM group cells by primary culture,the other group was not intervened with ADM and obtained control group cells by primary culture.Cell proliferation capacity was examined by single-cell clone formation assay.The expression of CD133 and ABCG2 were detected by flow cytometry and immunohistochemistry.Results The colony formation rate of ADM group was stronger than that of control group (P ＜0.05).Flow cytometry and immunohistochemistry showed that ABCG2 were positive in both groups,the expression rate of ABCG2 by flow cytometry was higher in ADM group(P ＜ 0.05),but the two methods indicated CD133 was negative in both groups.Conclusions The ADM group cells have certain characteristics of cancer stem cells.ABCG2 is associated with the formation of drug resistance in ACC and may be a potential therapeutic target.