CAJ | 학술논문

目的探讨皮肤黑素瘤及痣细胞痣组织中钠氢交换子调节因子-1 (NHERF1)和β-联蛋白表达的意义.方法收集47例皮肤黑素瘤及37例痣细胞痣组织,用免疫组化方法对NHERF1和β-联蛋白在组织中的表达模式(膜表达、浆表达及核表达)和表达强度进行检测.结果原位皮肤黑素瘤组织中NHERF1胞质中等/强阳性表达率为38％；而侵袭性皮肤黑素瘤组织中NHERF1胞质中等/强阳性表达率为71％,两组相比差异有统计学意义.同时,全部皮肤黑素瘤组织中NHERF1胞质中等/强阳性表达率为60％,而痣细胞痣组织中NHERF1胞质中等/强阳性表达率为0,差异有统计学意义.原位皮肤黑素瘤组织中可见β-联蛋白胞质中等/强阳性表达率为50％；而侵袭性皮肤黑素瘤组织中可见β-联蛋白胞质中等/强阳性表达率为84％.两组相比差异有统计学意义.全部皮肤黑素瘤组织中B一联蛋白胞质中等/强阳性表达率为72％,而痣细胞痣组织中NHERF1胞质中等/强阳性表达率为30％,两组相比具异有统计学意义.NHERF1及β-联蛋白胞质中等/强阳性表达率在痣细胞痣组、原位黑素瘤组及侵袭性黑素瘤组中均随着病变组织的恶性程度增加而升高.运用Spearman秩相关检验对皮肤黑素瘤中NHERF1及β-联蛋白的表达相关性进行分析显示无相关性.结论 NHERF1及β-联蛋白在痣细胞痣、原位黑素瘤及侵袭性黑素瘤组织中的胞质阳性表达随着病变组织的恶性程度增加而升高,但两者的表达差异无相关性.
목적 탐토피부흑소류급지세포지조직중납경교환자조절인자-1 (NHERF1)화β-련단백표체적의의.방법 수집47례피부흑소류급37례지세포지조직,용면역조화방법대NHERF1화β-련단백재조직중적표체모식(막표체、장표체급핵표체)화표체강도진행검측.결과 원위피부흑소류조직중NHERF1포질중등/강양성표체솔위38％；이침습성피부흑소류조직중NHERF1포질중등/강양성표체솔위71％,량조상비차이유통계학의의.동시,전부피부흑소류조직중NHERF1포질중등/강양성표체솔위60％,이지세포지조직중NHERF1포질중등/강양성표체솔위0,차이유통계학의의.원위피부흑소류조직중가견β-련단백포질중등/강양성표체솔위50％；이침습성피부흑소류조직중가견β-련단백포질중등/강양성표체솔위84％.량조상비차이유통계학의의.전부피부흑소류조직중B일련단백포질중등/강양성표체솔위72％,이지세포지조직중NHERF1포질중등/강양성표체솔위30％,량조상비구이유통계학의의.NHERF1급β-련단백포질중등/강양성표체솔재지세포지조、원위흑소류조급침습성흑소류조중균수착병변조직적악성정도증가이승고.운용Spearman질상관검험대피부흑소류중NHERF1급β-련단백적표체상관성진행분석현시무상관성.결론 NHERF1급β-련단백재지세포지、원위흑소류급침습성흑소류조직중적포질양성표체수착병변조직적악성정도증가이승고,단량자적표체차이무상관성.
Obiective To detect the expression of Na+/H+ exchanger regulatory factor 1 (NHERF1)and β-catenin in cutaneous melanoma and melanocytic nevus tissues.Methods Tissue samples were obtained from the lesions of 47 patients with cutaneous malignant melanoma and 37 patients with melanocytic nevi.Immunohistochemistry was used to determine the expression pattern (membrane,cytoplasmic or nuclear expression) and intensity of NHERF1 and β-catenin.Results The percentage of tissue samples with moderate/strong cytoplasmic expression of NHERF1 significantly differed between cutaneous melanoma in situ and invasive melanoma (38％ vs.71％,P ＜ 0.05),as well as between cutaneous melanoma and melanocytic nevi (60％ vs.0,P ＜ 0.05).A moderate/strong cytoplasmic expression of β-catenin was observed in 50％ of the cutaneous melanoma in situ tissue samples compared to 84％ of the invasive melanoma tissue samples (P ＜0.05),and 72％ of all the cutaneous melanoma tissue samples compared to 30％ of the melanocytic nevus tissue samples (P ＜ 0.05).The moderate/strong cytoplasmic expression rate of both NHERF1 and β-catenin in these tissue samples increased with the increase in degree of malignancy (i.e.,from melanocytic nevi,melanoma in situ to invasive melanoma).Spearman's rank correlation test revealed no correlation between the expression of NHERF1 and β-catenin in cutaneous melamoma tissues.Conclusions The moderate/strong cytoplasmic expression rate of both NHERF1 and β-catenin sequentially increases from melanocytic nevi,melanoma in situ to invasive melanoma,but there is no correlation between the expression of NHERF1 and β-catenin in cutaneous melamoma tissues.