国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2012年
6期
343-345,349,后插6
,共5页
董晖%余勤%刘丽珍%王艳%宣晓波%王标%单威%周丽萍%刘伟
董暉%餘勤%劉麗珍%王豔%宣曉波%王標%單威%週麗萍%劉偉
동휘%여근%류려진%왕염%선효파%왕표%단위%주려평%류위
粒细胞集落刺激因子%AMD3100%间充质干细胞%增殖%迁移%黏附
粒細胞集落刺激因子%AMD3100%間充質榦細胞%增殖%遷移%黏附
립세포집락자격인자%AMD3100%간충질간세포%증식%천이%점부
Granulocyte colony-stimulating factor%AMD3100%Mesenchymal stem cells%Proliferation%Migration%Adhesion
目的 探讨粒细胞集落刺激因子(G-CSF)、AMD3100对大鼠间充质干细胞(MSCs)增殖、迁移和黏附的影响.方法 采用MTT比色法、Transwell迁移法和黏附试验分别检测体外G-CSF、AMD3100对间充质干细胞的增殖、迁移和黏附能力的影响.结果 在G-CSF质量浓度为200 μg/L、AMD3100质量浓度为0.5 mg/L时MSCs增殖能力最强；在G-CSF质量浓度为200 μg/L、AMD3100质量浓度为0 mg/L时MSCs迁移能力最强；在G-CSF质量浓度为200 μg/L、AMD3100质量浓度为0.5 mg/L时MSCs黏附能力最强.结论 体外G-CSF对间充质干细胞的增殖、迁移和黏附能力呈促进作用,而AMD3100呈抑制作用.
目的 探討粒細胞集落刺激因子(G-CSF)、AMD3100對大鼠間充質榦細胞(MSCs)增殖、遷移和黏附的影響.方法 採用MTT比色法、Transwell遷移法和黏附試驗分彆檢測體外G-CSF、AMD3100對間充質榦細胞的增殖、遷移和黏附能力的影響.結果 在G-CSF質量濃度為200 μg/L、AMD3100質量濃度為0.5 mg/L時MSCs增殖能力最彊；在G-CSF質量濃度為200 μg/L、AMD3100質量濃度為0 mg/L時MSCs遷移能力最彊；在G-CSF質量濃度為200 μg/L、AMD3100質量濃度為0.5 mg/L時MSCs黏附能力最彊.結論 體外G-CSF對間充質榦細胞的增殖、遷移和黏附能力呈促進作用,而AMD3100呈抑製作用.
목적 탐토립세포집락자격인자(G-CSF)、AMD3100대대서간충질간세포(MSCs)증식、천이화점부적영향.방법 채용MTT비색법、Transwell천이법화점부시험분별검측체외G-CSF、AMD3100대간충질간세포적증식、천이화점부능력적영향.결과 재G-CSF질량농도위200 μg/L、AMD3100질량농도위0.5 mg/L시MSCs증식능력최강；재G-CSF질량농도위200 μg/L、AMD3100질량농도위0 mg/L시MSCs천이능력최강；재G-CSF질량농도위200 μg/L、AMD3100질량농도위0.5 mg/L시MSCs점부능력최강.결론 체외G-CSF대간충질간세포적증식、천이화점부능력정촉진작용,이AMD3100정억제작용.
Objective To study the effects ofgranulocyte colony-stimulating factor(G-CSF) and AMD3100 on the proliferation,migration and adhesion of mesenchymal stem cells (MSCs).Methods The proliferation,migration and adhesion of MSCs were detected by MTT chromometry,transwell and adhesion test.Results When the concentration of G-CSF was 200 μg/L and AMD3100 was 0.5 mg/L,the proliferation and migration of MSCs were the strongest.When the concentration of G-CSF was 200 μg/L and AMD3100 was 0 mg/L,the adhesion of MSCs was the strongest.Conclusion The proliferation,migration,adhesion of MSCs are promoted by G-CSF,and inhibited by AMD3100.
