CAJ | 학술논문

目的考察RGD多肽化学修饰聚苯乙烯培养板细胞相容性研究.方法采用高锰酸钾硫酸溶液对聚苯乙烯表面进行氧化反应,生成羧基位点；水溶性碳二亚胺活化羧基,接枝明胶、胶原和RGD多肽.用红外光谱、X-射线光电子能谱(XPS)、动态接触角等研究了RGD化学修饰聚苯乙烯的变化,并考察了RGD化学修饰聚苯乙烯后细胞相容性的变化.结果 XPS结果证实了聚苯乙烯表面N原子的引入,RGD是共价键合在聚苯乙烯表面的.动态接触角下降显著,证明修饰表面的亲水性能提高.在修饰后的聚苯乙烯培养板种植入表皮细胞,结果显示提高了细胞的黏附和增殖能力.结论聚苯乙烯表面进行RGD化学修饰,有利于提高细胞在其表面的黏附和增殖能力,有望为免疫磁珠分离得到的高纯度内皮祖细胞提供良好的培养介质.
목적 고찰RGD다태화학수식취분을희배양판세포상용성연구.방법 채용고맹산갑류산용액대취분을희표면진행양화반응,생성최기위점；수용성탄이아알활화최기,접지명효、효원화RGD다태.용홍외광보、X-사선광전자능보(XPS)、동태접촉각등연구료RGD화학수식취분을희적변화,병고찰료RGD화학수식취분을희후세포상용성적변화.결과 XPS결과증실료취분을희표면N원자적인입,RGD시공개건합재취분을희표면적.동태접촉각하강현저,증명수식표면적친수성능제고.재수식후적취분을희배양판충식입표피세포,결과현시제고료세포적점부화증식능력.결론 취분을희표면진행RGD화학수식,유리우제고세포재기표면적점부화증식능력,유망위면역자주분리득도적고순도내피조세포제공량호적배양개질.
Objective To investigate the cell compatibility of polystyrene(PS) plate chemically modified with RGD peptides.Methods PS surfaces were carboxylated by permanganate oxidation in diluted sulfuric acid,and carboxyls were activated with water-soluble carbodiimide to graft with gelatin,collagen and RGD peptides.IR,X-ray photo-electronic spectroscopy (XPS) and dynamic contact angle were used to characterize the surface modification of PS surface.Results XPS results confirmed the existence of nitrogen element from protein molecules and the covalently binding of proteins to PS surfaces.Dynamic contact angle measurement indicated hydrophilicity of PS surfaces was improved obviously after grafting modification.The cell culture results showed that the cell adhesion and proliferation was better on modified surfaces than the initial.Conclusion The cell compatibility of PS surface was great improved after modification with RGD peptides,which would provide a potential strategy to improve the culture of purified endothelial progenitor cells isolated by immunomagnetic beads.