国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2013年
1期
9-13,21
,共6页
张姝江%周秀苗%周嘉安%王迎军%陈艺
張姝江%週秀苗%週嘉安%王迎軍%陳藝
장주강%주수묘%주가안%왕영군%진예
软骨%组织工程%胶原%壳聚糖%聚谷氨酸苄酯-co-聚谷氨酸
軟骨%組織工程%膠原%殼聚糖%聚穀氨痠芐酯-co-聚穀氨痠
연골%조직공정%효원%각취당%취곡안산변지-co-취곡안산
Cartilage%Tissue engineering%Collagen%Chitosan%Poly-γ-benzyl-L-glutamate-co-glutamine acid
目的 利用聚谷氨酸苄酯-co-聚谷氨酸(PBLG-co-PGA)改性2种常规的生物可降解材料(胶原与壳聚糖),比较其性能及生物相容性,探索其用于软骨组织工程的可行性.方法 用PBLG-co-PGA改性胶原和壳聚糖,对改性前后的材料进行接触角、体外降解性能、拉伸强度、细胞黏附率及细胞相容性等性能的检测和比较.结果 随PBLG-co-PGA含量的增加,PBLG-co-PGA/胶原、PBLG-co-PGA/壳聚糖复合材料的降解速度减慢,接触角逐渐减小,PBLG-co-PGA/胶原复合材料的拉伸强度增加.选用PBLG-co-PGA质量分数为30％的复合材料,以兔软骨细胞进行细胞黏附率和细胞相容性实验,与无PBLG-co-PGA的单纯胶原或单纯壳聚糖材料比较,细胞黏附率差异无统计学意义(P＞0.05)；但兔软骨细胞在PBLG-co-PGA/壳聚糖复合材料上生长增殖优于其他3种材料(P＜0.05).结论 经PBLG-co-PGA改性后的壳聚糖和胶原材料,可以调节材料的降解速度,改善壳聚糖亲水性,增加胶原拉伸强度,并且有良好的细胞相容性.其中PBLG-co-PGA/壳聚糖复合材料具有更好的促细胞增殖特性,可以作为一种新的组织工程软骨支架材料.
目的 利用聚穀氨痠芐酯-co-聚穀氨痠(PBLG-co-PGA)改性2種常規的生物可降解材料(膠原與殼聚糖),比較其性能及生物相容性,探索其用于軟骨組織工程的可行性.方法 用PBLG-co-PGA改性膠原和殼聚糖,對改性前後的材料進行接觸角、體外降解性能、拉伸彊度、細胞黏附率及細胞相容性等性能的檢測和比較.結果 隨PBLG-co-PGA含量的增加,PBLG-co-PGA/膠原、PBLG-co-PGA/殼聚糖複閤材料的降解速度減慢,接觸角逐漸減小,PBLG-co-PGA/膠原複閤材料的拉伸彊度增加.選用PBLG-co-PGA質量分數為30％的複閤材料,以兔軟骨細胞進行細胞黏附率和細胞相容性實驗,與無PBLG-co-PGA的單純膠原或單純殼聚糖材料比較,細胞黏附率差異無統計學意義(P＞0.05)；但兔軟骨細胞在PBLG-co-PGA/殼聚糖複閤材料上生長增殖優于其他3種材料(P＜0.05).結論 經PBLG-co-PGA改性後的殼聚糖和膠原材料,可以調節材料的降解速度,改善殼聚糖親水性,增加膠原拉伸彊度,併且有良好的細胞相容性.其中PBLG-co-PGA/殼聚糖複閤材料具有更好的促細胞增殖特性,可以作為一種新的組織工程軟骨支架材料.
목적 이용취곡안산변지-co-취곡안산(PBLG-co-PGA)개성2충상규적생물가강해재료(효원여각취당),비교기성능급생물상용성,탐색기용우연골조직공정적가행성.방법 용PBLG-co-PGA개성효원화각취당,대개성전후적재료진행접촉각、체외강해성능、랍신강도、세포점부솔급세포상용성등성능적검측화비교.결과 수PBLG-co-PGA함량적증가,PBLG-co-PGA/효원、PBLG-co-PGA/각취당복합재료적강해속도감만,접촉각축점감소,PBLG-co-PGA/효원복합재료적랍신강도증가.선용PBLG-co-PGA질량분수위30％적복합재료,이토연골세포진행세포점부솔화세포상용성실험,여무PBLG-co-PGA적단순효원혹단순각취당재료비교,세포점부솔차이무통계학의의(P＞0.05)；단토연골세포재PBLG-co-PGA/각취당복합재료상생장증식우우기타3충재료(P＜0.05).결론 경PBLG-co-PGA개성후적각취당화효원재료,가이조절재료적강해속도,개선각취당친수성,증가효원랍신강도,병차유량호적세포상용성.기중PBLG-co-PGA/각취당복합재료구유경호적촉세포증식특성,가이작위일충신적조직공정연골지가재료.
Objective To prepare two types of biodegradable modified materials (chitosan and collagen)and evaluate whether the new materials are suitable for tissue engineering cartilage.Methods Collagen and chitosan were both modified by poly-γ-benzyl-L-glutamate-co-glutamine acid (PBLG-co-PGA) with different proportions.The contact angle,degradation rate,tensile strength,cell attachment and cytocompatibility were tested and compared.Results As the PBLG-co-PGA content varied,the degradation rates of PBLG-co-PGA composites became adjustable,the hydrophilicity of PBLG-co-PGA/chitosan was improved,and the tensile strength increased in PBLG-co-PGA/collagen composite.The composites with 30％ PBLG-co-PGA were chosen for cytocompatibility and cell attachment experiments.The rabbit condrocytes grew significantly better on PBLG-co-PGA/chitosan than on other three materials(P＜0.05).Conclusion PBLG can improve the hydrophilicity,tensile strength and regulate the degradation rate of composite materials,and the cytocompatibility of the composites with 30％ of PBLG is good,among which PBLG-co-PGA/chitosan can even promote cell proliferation.It could be a new choice of scaffold for tissue engineering cartilage.
