国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2013年
3期
139-142,164,封3
,共6页
洪阁%郭江红%杨晓姣%刘天军
洪閣%郭江紅%楊曉姣%劉天軍
홍각%곽강홍%양효교%류천군
亚氨基二乙酸修饰四苯基卟啉%光动力疗法%肝癌H22%脂水分配系数%单线态氧
亞氨基二乙痠脩飾四苯基卟啉%光動力療法%肝癌H22%脂水分配繫數%單線態氧
아안기이을산수식사분기계람%광동력요법%간암H22%지수분배계수%단선태양
Iminodiacetic acid modified tetraphenylporphyrin%Photodynamic therapy%H22 liver cancer%Octanol-water partition coefficient%Singlet oxygen
目的 评价本实验室自行合成的亚氨基二乙酸修饰四苯基卟啉光敏剂的理化性质及对H22小鼠肝癌的光动力治疗作用.方法 采用紫外分光光度法测定亚氨基二乙酸修饰四苯基卟啉光敏剂的紫外吸收光谱、脂水分配系数和单线态氧产率;以肝癌H22小鼠为研究对象,随机分为空白对照组、光照组、光敏剂组、光动力治疗1次组和光动力治疗2次组,考察亚氨基二乙酸修饰四苯基卟啉光敏剂介导的光动力疗法(PDT)对实体瘤的抑制率和荷瘤动物免疫机能的影响.结果 亚氨基二乙酸修饰四苯基卟啉光敏剂在650 nm波长处存在紫外吸收,脂水分配系数和单线态氧产率分别为2.30和0.52;单纯激光照射和单纯给予亚氨基二乙酸修饰四苯基卟啉光敏剂对肝癌H22小鼠无治疗作用(P＞0.05),而亚氨基二乙酸修饰四苯基卟啉光敏剂介导的光动力疗法对小鼠肝癌H22的生长具有极其显著的抑制作用(P＜0.001),光动力治疗2次组肿瘤抑制率高达95.15％.各治疗组对荷瘤小鼠的体质量增长、肝、脾、肺、胸腺指数几乎无影响.结论 亚氨基二乙酸修饰四苯基卟啉光敏剂单线态氧产率高、两亲性好,介导的PDT抗肿瘤活性强,适合作为光动力抗肿瘤候选新药进行开发.
目的 評價本實驗室自行閤成的亞氨基二乙痠脩飾四苯基卟啉光敏劑的理化性質及對H22小鼠肝癌的光動力治療作用.方法 採用紫外分光光度法測定亞氨基二乙痠脩飾四苯基卟啉光敏劑的紫外吸收光譜、脂水分配繫數和單線態氧產率;以肝癌H22小鼠為研究對象,隨機分為空白對照組、光照組、光敏劑組、光動力治療1次組和光動力治療2次組,攷察亞氨基二乙痠脩飾四苯基卟啉光敏劑介導的光動力療法(PDT)對實體瘤的抑製率和荷瘤動物免疫機能的影響.結果 亞氨基二乙痠脩飾四苯基卟啉光敏劑在650 nm波長處存在紫外吸收,脂水分配繫數和單線態氧產率分彆為2.30和0.52;單純激光照射和單純給予亞氨基二乙痠脩飾四苯基卟啉光敏劑對肝癌H22小鼠無治療作用(P＞0.05),而亞氨基二乙痠脩飾四苯基卟啉光敏劑介導的光動力療法對小鼠肝癌H22的生長具有極其顯著的抑製作用(P＜0.001),光動力治療2次組腫瘤抑製率高達95.15％.各治療組對荷瘤小鼠的體質量增長、肝、脾、肺、胸腺指數幾乎無影響.結論 亞氨基二乙痠脩飾四苯基卟啉光敏劑單線態氧產率高、兩親性好,介導的PDT抗腫瘤活性彊,適閤作為光動力抗腫瘤候選新藥進行開髮.
목적 평개본실험실자행합성적아안기이을산수식사분기계람광민제적이화성질급대H22소서간암적광동력치료작용.방법 채용자외분광광도법측정아안기이을산수식사분기계람광민제적자외흡수광보、지수분배계수화단선태양산솔;이간암H22소서위연구대상,수궤분위공백대조조、광조조、광민제조、광동력치료1차조화광동력치료2차조,고찰아안기이을산수식사분기계람광민제개도적광동력요법(PDT)대실체류적억제솔화하류동물면역궤능적영향.결과 아안기이을산수식사분기계람광민제재650 nm파장처존재자외흡수,지수분배계수화단선태양산솔분별위2.30화0.52;단순격광조사화단순급여아안기이을산수식사분기계람광민제대간암H22소서무치료작용(P＞0.05),이아안기이을산수식사분기계람광민제개도적광동력요법대소서간암H22적생장구유겁기현저적억제작용(P＜0.001),광동력치료2차조종류억제솔고체95.15％.각치료조대하류소서적체질량증장、간、비、폐、흉선지수궤호무영향.결론 아안기이을산수식사분기계람광민제단선태양산솔고、량친성호,개도적PDT항종류활성강,괄합작위광동력항종류후선신약진행개발.
Objective To explore the physical/chemical properties and effect of photodynamic therapy (PDT) of iminodiacetic acid modified tetraphenylporphyrin on mice bearing H22 liver cancer.Methods The UV absorbance spectrum,singlet oxygen yield and lipid-water partition coefficient were measured by UV spectrophotometry.Mice bearing H22 liver cancer as animal model were divided into control group (no drug,no light),light treated group (no drug,only light),drug treated group (only drug,no light),experimental once group (with drug and light,once) and experimental twice group (with drug and light,twice,PDT twice).Tumor inhibition rate and index of liver,spleen,lung,thymus of the mice were calculated to evaluate the antitumor activity of iminodiacetic acid modified tetraphenyl porphyrin-PDT.Results The iminodiacetic acid modified tetraphenylporphyrin had absorption at 650 nm,while its singlet oxygen yield and lipid-water partition coefficient were 2.30 and 0.52,respectively.No antitumor effect on mice bearing H22liver cancer for light treated group and drug treated group respectively.However,significant antitumor effect (P＜0.001)was found at experimental group.The maximum tumor inhibition rate could be 95.15％ for PDT twice group.No significant differences were observed on weight gain and liver,lung,spleen,thymus index in each group.Conclusion Iminodiacetic acid modified tetraphenylporphyrin with high singlet oxygen yield,low toxicity and significant in vivo anti-tumor activity,is suitable to be developed as an anti-tumor drug candidate for photodynamic therapy.
