CAJ | 학술논문

目的将骨形态发生蛋白-2(BMP-2)活性多肽P24复合于聚三甲基碳酸酯-(聚氧乙烯-聚氧丙烯-聚氧乙烯)-聚三甲基碳酸酯(PTMC11-F127-PTMC11)凝胶材料上,测定BMP-2活性多肽P24体外释放曲线是否符合缓释要求.载P24/PTMC11-F127-PTMC11凝胶植入大鼠骶棘肌内,观察异位成骨情况.方法将P24复合于不同质量分数(16％,20％,25％)的PTMC11-F127-PTMC11凝胶材料成形后,二喹啉甲酸(BCA)法测定释放的多肽;将载P24/PTMC11-F127-PTMC11凝胶植于大鼠骶棘肌,行组织学切片HE染色检测.结果根据BCA法检测结果绘制释放曲线分析,质量分数为16％载P24/PTMC11-F127-PTMC11凝胶突释效应较质量分数为20％、25％载P24/PTMC11-F127-PTMC11凝胶突释效应大,后2者在突释效应后释效均匀,可持续释放1个月左右,累积释放97.4％.第4周取材行组织学切片HE染色示一定量不成熟、散在的针状骨小梁组织;第6周可见骨小梁增宽增粗,骨陷窝细胞增多.结论载P24/PTMC11-F127-PTMC11凝胶体外释放曲线符合缓释要求.载P24/PTMC11-F127-PTMC11凝胶在大鼠体内能平稳释放活性多肽,使BMP-2活性多肽在体内能发挥其诱导成骨活性,PTMC11-F127-PTMC11凝胶在体内引起炎症反应少,生物相容性良好,可作为骨形态发生蛋白-2活性多肽合适的载体材料.
목적 장골형태발생단백-2(BMP-2)활성다태P24복합우취삼갑기탄산지-(취양을희-취양병희-취양을희)-취삼갑기탄산지(PTMC11-F127-PTMC11)응효재료상,측정BMP-2활성다태P24체외석방곡선시부부합완석요구.재P24/PTMC11-F127-PTMC11응효식입대서저극기내,관찰이위성골정황.방법 장P24복합우불동질량분수(16％,20％,25％)적PTMC11-F127-PTMC11응효재료성형후,이규람갑산(BCA)법측정석방적다태;장재P24/PTMC11-F127-PTMC11응효식우대서저극기,행조직학절편HE염색검측.결과 근거BCA법검측결과회제석방곡선분석,질량분수위16％재P24/PTMC11-F127-PTMC11응효돌석효응교질량분수위20％、25％재P24/PTMC11-F127-PTMC11응효돌석효응대,후2자재돌석효응후석효균균,가지속석방1개월좌우,루적석방97.4％.제4주취재행조직학절편HE염색시일정량불성숙、산재적침상골소량조직;제6주가견골소량증관증조,골함와세포증다.결론 재P24/PTMC11-F127-PTMC11응효체외석방곡선부합완석요구.재P24/PTMC11-F127-PTMC11응효재대서체내능평은석방활성다태,사BMP-2활성다태재체내능발휘기유도성골활성,PTMC11-F127-PTMC11응효재체내인기염증반응소,생물상용성량호,가작위골형태발생단백-2활성다태합괄적재체재료.
Objective To prepare P24/PTMC11-F127-PTMC11 hydrogel,to study the in vitro release profile and to observe ectopic bone formation in p24 peptide incorporated PTMC11-F127-PTMC11 hydrogel.Methods Corresponding weight powder of p24 peptide was infunded into tubes of PTMC11-F127-PTMC11 solution with concentrations of 16％,20％ and 25％.Release profiles of P24 peptide in different concentration PTMC11-F127-PTMC11 hydrogel were measured in vitro by BCA assay.P24/PTMC11-F127-PTMC11 hydrogel was implanted into each rat's erector muscle of spine,and the implanted gel was detected by hematoxylin and eosin stain (HE).Results PTMC11-F127-PTMC11 hydrogel showed sustained slow release for the whole process after the initial burst release.With the increase of concentration in PTMC11-F127-PTMC.hydrogel,the initial burst release was reduced significantly.Ectopic bone formation was observed by computed tomography in p24 peptide incorporated PTMC11-F127-PTMC11 hydrogel after four weeks.Bone trabeculae surround the P24/PTMC11-F127-PTMC11 hydrogel was observed at forth week by hematoxylin and eosin stain.The bone trabeculae became thicker from sixth week.Conclusion Delayed release of peptide from the hydrogel was mainly controlled by disintegration of hydrogel and a satisfactory release profile was observed.These results suggest that the p24-loaded PTMC11-F127-PTMC11 hydrogel remmns active of p24 at the implanted site,continuously induce differentiation of osteoblast precursor cells into osteoblasts,and activate osteoblasts to promote ectopic calcification.