CAJ | 학술논문

目的研制低分子肝素钠(LMWH)涂层和多醛基氧化海藻酸钠(OSA)涂层的聚碳酸酯,并对其生物相容性进行评价.方法利用化学接枝改性方法制备涂层材料,进行表征并验证涂层稳定性;通过接触角测定、体外血清蛋白、血小板黏附试验、体外凝血试验等进行生物相容性评价.结果分析红外光谱,发现涂层后材料出现与涂层物相关的吸收峰变化,定量结果显示材料表面涂层量稳定.与未涂层材料相比,涂层材料表面与水的接触角减小,血清蛋白、血小板黏附量显著减少,凝血酶原时间(PT)和活化部分凝血活酶时间(APTT)显著延长,其差异有统计学意义(P＜0.05).与LMWH涂层材料相比,OSA涂层材料抗凝效果略差,但其蛋白黏附量、表面接触角小于LMWH涂层,差异具有统计学意义(P＜0.05).结论应用化学接枝改性方法制备LMWH、OSA涂层的聚碳酸酯,其涂层稳定,生物相容性显著改善.
목적 연제저분자간소납(LMWH)도층화다철기양화해조산납(OSA)도층적취탄산지,병대기생물상용성진행평개.방법 이용화학접지개성방법제비도층재료,진행표정병험증도층은정성;통과접촉각측정、체외혈청단백、혈소판점부시험、체외응혈시험등진행생물상용성평개.결과 분석홍외광보,발현도층후재료출현여도층물상관적흡수봉변화,정량결과현시재료표면도층량은정.여미도층재료상비,도층재료표면여수적접촉각감소,혈청단백、혈소판점부량현저감소,응혈매원시간(PT)화활화부분응혈활매시간(APTT)현저연장,기차이유통계학의의(P＜0.05).여LMWH도층재료상비,OSA도층재료항응효과략차,단기단백점부량、표면접촉각소우LMWH도층,차이구유통계학의의(P＜0.05).결론 응용화학접지개성방법제비LMWH、OSA도층적취탄산지,기도층은정,생물상용성현저개선.
Objective To prepare and evaluate the biocompatibility of polycarbonate coated with low molecular weight heparin (LMWH) and partial oxidation sodium alginate(OSA).Methods Coating material was prepared by means of chemical graft-modification and the feature of the material was determined with infrared spectrum and the stablity of the coating in fluid was examined.Biocompatibility was evaluated by contact angle and in-vitro tests including protein adhesion,platelet adhesion and caugulation.Results LMWH or OSA was tightly combined with polycarbonate.After being coated,the contact angle,albumin and fibrinogen adhering to materials were decrease (P＜0.05).The anticoagulant activity was notably promoted by coating.Compared with LMWH coated material,the contact angle,albumin and fibrinogen adhering were decreased significantly,but the improvement of anticaugulation was limited(P＜0.05).Conclusion Chemical graft-modification LMWH or OSA can be applied to polycarbonate.The biocompatibility of the coated materials was significantly promoted.