CAJ | 학술논문

目的探讨人p66Shc重组腺病毒抑制人宫颈癌Hela细胞增殖的作用及机制.方法 Hela细胞分为空白对照组(不感染腺病毒)、阴性对照组(感染Adeno X-LacZ)和实验组(感染AdenoX-p66Shc).通过细胞生长曲线、MTT检测分析观察细胞的生长状态；DHE染色法检测细胞内活性氧生成；流式细胞仪检测细胞周期；Western Blot法检测细胞内相关蛋白的表达.结果 p66Shc重组腺病毒能够抑制Hela细胞增殖,且随着p66Shc重组腺病毒处理时间延长和剂量增加其抑制作用增强.与空白和阴性对照组比较,p66Shc重组腺病毒感染48 h后,G2/M期细胞比例明显升高,其差异具有统计学意义(P＜0.01)；同时引起Hela细胞活性氧水平显著上升,p53、CyclinB1蛋白表达显著升高,其差异具有统计学意义(P＜0.05).结论 p66Shc重组腺病毒能够显著抑制Hela细胞增殖,并诱导其发生G2/M期阻滞.
목적 탐토인p66Shc중조선병독억제인궁경암Hela세포증식적작용급궤제.방법 Hela세포분위공백대조조(불감염선병독)、음성대조조(감염Adeno X-LacZ)화실험조(감염AdenoX-p66Shc).통과세포생장곡선、MTT검측분석관찰세포적생장상태；DHE염색법검측세포내활성양생성；류식세포의검측세포주기；Western Blot법검측세포내상관단백적표체.결과 p66Shc중조선병독능구억제Hela세포증식,차수착p66Shc중조선병독처리시간연장화제량증가기억제작용증강.여공백화음성대조조비교,p66Shc중조선병독감염48 h후,G2/M기세포비례명현승고,기차이구유통계학의의(P＜0.01)；동시인기Hela세포활성양수평현저상승,p53、CyclinB1단백표체현저승고,기차이구유통계학의의(P＜0.05).결론 p66Shc중조선병독능구현저억제Hela세포증식,병유도기발생G2/M기조체.
Objective To investigate the inhibitive effect and mechanism of human p66Shc recombinant adenovirus on the proliferation of human cervical cancer Hela cells.Methods The cells were divided into three groups:blank control group (Hela cells),negative control group (Hela cells infected with Adeno X-LacZ) and experiment group(Hela cells infected with Adeno X-p66Shc).The proliferation ability was investigated by cell growth curve,MTT detection.Reactive oxygen species (ROS) were detected by Fluorescent Probe-DHE(dihydroethidium).The cell cycles were analyzed by flow cytometry.Western blot were used to observe associated protein expression.Results The human p66Shc recombinant adenovirus could efficiently suppress the proliferation of Hela cells.The inhibition effects seem to be positive dose and time of action dependent.Compared with blank and negative control group,the ratio of the cell phase G2/M of Hela cells infected with Adeno X-p66Shc for 48h showed a significant increase.The cellular ROS,the expression of p53 and Cyclin B 1 proteins were upregulated in Hela cells.Conclusion Adeno X-p66Shc can effectively inhibit proliferation of Hela cells and induce cell cycle arrest at G2/M phase.