国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2013年
4期
216-219,后插4-后插5
,共5页
赵传艳%王昕%张春亮%曹清涛
趙傳豔%王昕%張春亮%曹清濤
조전염%왕흔%장춘량%조청도
管家基因%GeNorm程序%基因表达
管傢基因%GeNorm程序%基因錶達
관가기인%GeNorm정서%기인표체
Housekeeping gene%GeNorm program%Gene expression
目的 反转录聚合酶链反应(RT-PCR)广泛应用于基因表达分析,其中选择合适的管家基因非常重要.大鼠心肌梗死模型是心肌梗死相关研究的主要动物模型,而心肌梗死后大鼠管家基因选择问题研究报道则较少.本研究拟探讨大鼠心梗模型中管家基因表达的稳定性.方法 结扎冠脉前降支制作心梗模型,分析并挑选出使用广泛的4个标准管家基因:3-磷酸甘油醛脱氢酶(GAPDH),核糖体蛋白L13A(RPL 13A),β-肌动蛋白(ACTB),结合区连接蛋白(ARBP);用实时荧光反转录-聚合酶链反应方法比较它们在心梗大鼠心脏中的表达,采用GeNorm程序分析最适于心肌梗死后大鼠基因表达管家基因研究.结果 RPL13A、GAPDH、ARBP及ACTB基因表达稳定度的平均值(M值)分别为0.812、0.721、0.812和1.2.结论 GAPDH及ARBP是大鼠心梗模型中表达最稳定的基因.
目的 反轉錄聚閤酶鏈反應(RT-PCR)廣汎應用于基因錶達分析,其中選擇閤適的管傢基因非常重要.大鼠心肌梗死模型是心肌梗死相關研究的主要動物模型,而心肌梗死後大鼠管傢基因選擇問題研究報道則較少.本研究擬探討大鼠心梗模型中管傢基因錶達的穩定性.方法 結扎冠脈前降支製作心梗模型,分析併挑選齣使用廣汎的4箇標準管傢基因:3-燐痠甘油醛脫氫酶(GAPDH),覈糖體蛋白L13A(RPL 13A),β-肌動蛋白(ACTB),結閤區連接蛋白(ARBP);用實時熒光反轉錄-聚閤酶鏈反應方法比較它們在心梗大鼠心髒中的錶達,採用GeNorm程序分析最適于心肌梗死後大鼠基因錶達管傢基因研究.結果 RPL13A、GAPDH、ARBP及ACTB基因錶達穩定度的平均值(M值)分彆為0.812、0.721、0.812和1.2.結論 GAPDH及ARBP是大鼠心梗模型中錶達最穩定的基因.
목적 반전록취합매련반응(RT-PCR)엄범응용우기인표체분석,기중선택합괄적관가기인비상중요.대서심기경사모형시심기경사상관연구적주요동물모형,이심기경사후대서관가기인선택문제연구보도칙교소.본연구의탐토대서심경모형중관가기인표체적은정성.방법 결찰관맥전강지제작심경모형,분석병도선출사용엄범적4개표준관가기인:3-린산감유철탈경매(GAPDH),핵당체단백L13A(RPL 13A),β-기동단백(ACTB),결합구련접단백(ARBP);용실시형광반전록-취합매련반응방법비교타문재심경대서심장중적표체,채용GeNorm정서분석최괄우심기경사후대서기인표체관가기인연구.결과 RPL13A、GAPDH、ARBP급ACTB기인표체은정도적평균치(M치)분별위0.812、0.721、0.812화1.2.결론 GAPDH급ARBP시대서심경모형중표체최은정적기인.
Objective Reverse transcription-polymerase chain reaction (RT-PCR) is widely used in gene expression analysis.Selection of proper housekeeping gene is very important.Rat model of myocardial infarction is a major animal model of myocardial infarction.Few reports are found about the selection of rat housekeeping gene after myocardial infarction.This study was aimed to explore the stability of the housekeeping genes expression in the rat model of myocardial infarction.Methods Myocardial infarction models were made using the anterior descending coronary artery ligation method.Combining with practical work,analysis and selection were made of four widely used standard housekeeping genes:glyceraldehydes-3-phosphate dehydrogenase (GAPDH),ribosomal protein L13A (RPL13A),beta-actin (ACTB) and acidic ribosomal phosphoprotein P0 (ARBP).The expressions in the rat heart were compared using real-time fluorescent RT-PCR and analysis was carried out to figure out which was the most suitable housekeeping gene for study of gene expression in rats after myocardial infarction using GeNorm program.Results The M values of RPL13A,GAPDH,ARBP and ACTB gene were 0.812,0.721,0.812 and 1.2 respectively.Conclusion GAPDH and ARBP are the most stable genes for the rat myocardial infarction model.
