国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2013年
6期
324-327,343,后插2
,共6页
于湛%于敏%周志敏%张之宝%杜博%张哲%熊青青%孙建军
于湛%于敏%週誌敏%張之寶%杜博%張哲%熊青青%孫建軍
우담%우민%주지민%장지보%두박%장철%웅청청%손건군
牛血清白蛋白%纳米粒%缓释载体%圆窗膜%内耳
牛血清白蛋白%納米粒%緩釋載體%圓窗膜%內耳
우혈청백단백%납미립%완석재체%원창막%내이
Bovine serum albumin%Nanoparticle%Controlled release carrier,Round window membrane%Inner ear
目的 目前在临床上国内外尚无对内耳病局部用药的缓释剂,本研究旨在探讨能否将白蛋白纳米粒载体材料作为鼓室跨膜给药缓释剂.方法 采用去溶剂化法制备空白白蛋白纳米粒并进行系统表征和细胞毒性评价.为便于观察,选取一种红色荧光染料即罗丹明B(RhB)作为模型药物,以物理吸附方式与空白白蛋白纳米粒结合形成载药白蛋白纳米粒,测定其载药量、包封率及体外药物释放曲线,同时采用小动物活体成像技术观察其注入豚鼠听泡内跨圆窗膜转运扩散情况.结果 制备的白蛋白纳米粒为实心球形,表面光滑,平均粒径大小为476 nm,Zeta电位为15.4 mV.体外药物释放结果表明,该纳米粒具有缓释效果.经戊二醛交联固定的白蛋白纳米粒具有一定的细胞毒性;而经热变性处理的白蛋白纳米粒具有较好的细胞相容性.小动物活体成像实验可以看到RhB在听泡内滞留扩散,而后经解剖观察,证明白蛋白纳米粒可在圆窗膜表面附着并穿越圆窗膜实现跨膜向耳蜗内转运.结论 制备的白蛋白纳米粒结构完整,制备方法简单、无毒性,可以很好地包载药物并具有缓释功能,为进一步制备可注射跨圆窗膜定向缓释纳米凝胶奠定了坚实的基础.
目的 目前在臨床上國內外尚無對內耳病跼部用藥的緩釋劑,本研究旨在探討能否將白蛋白納米粒載體材料作為鼓室跨膜給藥緩釋劑.方法 採用去溶劑化法製備空白白蛋白納米粒併進行繫統錶徵和細胞毒性評價.為便于觀察,選取一種紅色熒光染料即囉丹明B(RhB)作為模型藥物,以物理吸附方式與空白白蛋白納米粒結閤形成載藥白蛋白納米粒,測定其載藥量、包封率及體外藥物釋放麯線,同時採用小動物活體成像技術觀察其註入豚鼠聽泡內跨圓窗膜轉運擴散情況.結果 製備的白蛋白納米粒為實心毬形,錶麵光滑,平均粒徑大小為476 nm,Zeta電位為15.4 mV.體外藥物釋放結果錶明,該納米粒具有緩釋效果.經戊二醛交聯固定的白蛋白納米粒具有一定的細胞毒性;而經熱變性處理的白蛋白納米粒具有較好的細胞相容性.小動物活體成像實驗可以看到RhB在聽泡內滯留擴散,而後經解剖觀察,證明白蛋白納米粒可在圓窗膜錶麵附著併穿越圓窗膜實現跨膜嚮耳蝸內轉運.結論 製備的白蛋白納米粒結構完整,製備方法簡單、無毒性,可以很好地包載藥物併具有緩釋功能,為進一步製備可註射跨圓窗膜定嚮緩釋納米凝膠奠定瞭堅實的基礎.
목적 목전재림상상국내외상무대내이병국부용약적완석제,본연구지재탐토능부장백단백납미립재체재료작위고실과막급약완석제.방법 채용거용제화법제비공백백단백납미립병진행계통표정화세포독성평개.위편우관찰,선취일충홍색형광염료즉라단명B(RhB)작위모형약물,이물리흡부방식여공백백단백납미립결합형성재약백단백납미립,측정기재약량、포봉솔급체외약물석방곡선,동시채용소동물활체성상기술관찰기주입돈서은포내과원창막전운확산정황.결과 제비적백단백납미립위실심구형,표면광활,평균립경대소위476 nm,Zeta전위위15.4 mV.체외약물석방결과표명,해납미립구유완석효과.경무이철교련고정적백단백납미립구유일정적세포독성;이경열변성처리적백단백납미립구유교호적세포상용성.소동물활체성상실험가이간도RhB재은포내체류확산,이후경해부관찰,증명백단백납미립가재원창막표면부착병천월원창막실현과막향이와내전운.결론 제비적백단백납미립결구완정,제비방법간단、무독성,가이흔호지포재약물병구유완석공능,위진일보제비가주사과원창막정향완석납미응효전정료견실적기출.
Objective To study the clinical application of local drug control-release,modified bovine serum albumin (BSA) nanoparticles was used as controlled release carrier for local drug delivery to the inner ear.Methods Modified BSA nanoparticles,with or without Rhodamine B (RhB),were prepared by improved desolvation method and be confirmed by electron microscope and particle size analyzer.Drug loading,encapsulation efficiency and drug release were analyzed.L929 cells were used for cell biocompatibility assay.Nanoparticles with RhB was used for in vivo imaging to investigate the penetration of particles into the round window membrane (RWM).Results The BSA nanoparticles were smooth-surfaced solid-spheres with average diameter of 476 nm.The Zetapotential of blank nanoparticle was 15.4 mV.RhB,a model drug,was proved to be adsorbed on the nanoparticles and to be release control.Glutaraldehyde cross-linked BSA nanoparticles had certain cytotoxicity,while heat denatured BSA nanoparticles had good cell compatibility.Nanoparticles with RhB was proved to be adsorbed on and penetrated through the RWM.Conclusion The nanoparticles have complete structure and carry drug well for control-release.The preparation is simple and non-toxic.The outcome provides the basis for the study of injectable cross RWM control-release nanogel.
